碧云天新增高品质蛋白标签抗体和内参抗体

高灵敏度、高特异性、低价高效!

想要搞好科研,发高分文章?
寻找信号通路,研究错综复杂的分子机制必不可少!
Western Blot、ELISA、IP、免疫组化、免疫荧光等都是目前研究分子机制的常用方法。
这些实验,似乎哪一个都跟抗体有关。所以,选择一个好用的抗体非常关键。
但是,同一个蛋白抗体,每个公司都有,不知哪家好?
一个进口抗体,动辄就要2000起!
面临着纷呈复杂的抗体试剂市场,如何选择到适合自己实验的抗体呢?
只买对的,不买贵的,买抗体,来碧云天!

碧云天在现有多种抗体的基础上,全新推出了千种高品质、高灵敏度、高特异性抗体,更有KO验证的高特异性抗体,每种抗体都经过精心测试,确保检测效果!
接下来,我们将分多期进行介绍最新上线的抗体,请大家持续关注。

首先是碧云天高品质蛋白标签抗体
蛋白标签是指利用DNA体外重组技术,与目的蛋白一起表达的一种多肽或蛋白,以便于目的蛋白的表达、检测、示踪和纯化等。常用的蛋白标签包括Flag、Myc、HA、His、GST、GFP等。带上蛋白标签后,通常不会影响目标蛋白的生物活性和细胞内定位。但检测和蛋白标签融合表达的目标蛋白时,使用商业化的相应抗体就可以完成,大大方便了实验检测。

产品信息:

产品编号 产品名称 包装 产品价格
AF5051 Flag Tag Mouse Monoclonal Antibody 50µl
AF5054 Myc Tag Mouse Monoclonal Antibody 50µl
AF5057 HA Tag Mouse Monoclonal Antibody 50µl
AF5060 His Tag Mouse Monoclonal Antibody 50µl
AF5063 GST Tag Mouse Monoclonal Antibody 50µl
AF5066 GFP Tag Rabbit Polyclonal Antibody 50µl

检测效果:


a. Myc Tag Mouse Monoclonal Antibody(AF5054)


b.His Tag Mouse Monoclonal Antibody (AF5060)

Fig1. Western blot analysis of over-expressed Myc-tagged protein(a)and His-tagged protein(b) in 293T cells using Beyotime antibody at different dilution. Each lane was loaded with 2µg cell lysate. Secondary antibody: HRP-labeled Goat Anti-Mouse IgG(H+L) (A0216) at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A). Exposure time: 30s


a.Flag Tag Mouse Monoclonal Antibody (AF5051)


b.His Tag Mouse Monoclonal Antibody (AF5060)

Fig2. Immunofluorescence analysis of 293T cells using Beyotime Antibody. Blue: DAPI for nuclear staining.


a.Myc Tag Mouse Monoclonal Antibody(AF5054)


b.HA Tag Mouse Monoclonal Antibody (AF5057)

Fig3. Immunoprecipitation of over-expressed Myc-tagged protein(a) and HA-tagged protein(b) in 293T cells incubated using Beyotime antibody. A mock served as negative control and over-expressed 293T cell lysate served as positive control.


Fig4. Immunofluorescence analysis of GFP transgenic 293T cells using GFP Tag Rabbit Polyclonal Antibody(AF5066). Green: GFP expression. Blue: DAPI for nuclear staining.

相关产品:
还有更多高品质蛋白标签抗体供您挑选,可以根据您的实验方法和需求进行挑选。

产品编号 产品名称 包装 产品价格
AF5051 Flag Tag Mouse Monoclonal Antibody 50µl 398.00元
AF5054 Myc Tag Mouse Monoclonal Antibody 50µl 398.00元
AF5057 HA Tag Mouse Monoclonal Antibody 50µl 398.00元
AF5060 His Tag Mouse Monoclonal Antibody 50µl 398.00元
AF5063 GST Tag Mouse Monoclonal Antibody 50µl 398.00元
AF5066 GFP Tag Rabbit Polyclonal Antibody 50µl 398.00元
AF0033 Myc Tag Mouse Monoclonal Antibody 100μl
AF0036 FLAG Tag Rabbit Polyclonal Antibody 100μl
AF0039 HA Tag Rabbit Polyclonal Antibody 100μl
AF0051 AU1-tag Mouse Monoclonal Antibody 50μl
AF0135 E-tag Mouse Monoclonal Antibody 50μl
AF0159 GFP Mouse Monoclonal Antibody 50μl
AF0174 GST Mouse Monoclonal Antibody 50μl
AF0240 MuERVL-Gag Rabbit Polyclonal Antibody 50μl
AF0285 S-tag Mouse Monoclonal Antibody 50μl
AF0294 T7-tag Mouse Monoclonal Antibody 50μl
AF1225 Maltose Binding Protein Rabbit Monoclonal Antibody 50μl
AF1354 FUBP1 Rabbit Monoclonal Antibody 50μl
AF1483 GFP Rabbit Monoclonal Antibody 50μl
AF2299 GST Rabbit Monoclonal Antibody 50μl
AF2305 HA tag Rabbit Monoclonal Antibody 50μl
AF519 Flag抗体(小鼠单抗) >40次
AG279 GFP抗体(兔多抗) >40次
AG281 GFP抗体(小鼠单抗) >40次
AG768 GST抗体(小鼠单抗) >20次
AH158 HA抗体(小鼠单抗) >40次
AH367 His-tag抗体(小鼠单抗) >20次
AM926 Myc抗体(小鼠单抗) >30次
AM933 Myc抗体(兔单抗) >20次

接下来是碧云天高品质内参抗体。
我们知道,用WB比较不同细胞或组织中目的蛋白表达量的差异时,需要选择参照物。WB实验中的参照物即内参,内参一般选用管家基因表达的蛋白,在各组织和细胞中的表达相对恒定,可以通过检测每个样品内参的量来校正上样误差,这样半定量的结果才更为可信。此外使用内参可以检测WB过程是否正常。

产品信息:

产品编号 产品名称 包装 产品价格
AF5001 β-Actin Mouse Monoclonal Antibody 50µl 398.00元
AF5003 β-Actin Rabbit Monoclonal Antibody 50µl 398.00元
AF5006 β-Actin-HRP Rabbit Monoclonal Antibody 50µl 458.00元
AF5009 GAPDH Mouse Monoclonal Antibody 50µl 398.00元
AF5012 α-Tubulin Rabbit Polyclonal Antibody 50µl 398.00元

检测效果:


Fig1. Western blot analysis of extracts of various cell lines, using β-Actin Rabbit Monoclonal Antibody(AF5003) at 1:2000 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A). Exposure time: 30s.


Fig2. Immunohistochemistry of paraffin-embedded rat spleen using GAPDH antibody(AF5009) at dilution of 1:50 (40x lens).


Fig3. Immunohistochemistry of paraffin-embedded human stomach using β-Actin Mouse Monoclonal Antibody(AF5001) at dilution of 1:50 (40x lens).


Fig4. Immunofluorescence analysis of HeLa cells using β-Actin Rabbit Monoclonal Antibody(AF5003)

小编温馨提示,β-Actin-HRP Rabbit Monoclonal Antibody(AF5006)自带HRP标签,与内参蛋白反应后无需孵育二抗即可显色,特异性更高。
此外,碧云天还有多种内参抗体,可以根据您的样本来源、检测蛋白的分子量和表达部位按需选择。

相关产品:

产品编号 产品名称 包装 产品价格
AF5001 β-Actin Mouse Monoclonal Antibody 50µl 398.00元
AF5003 β-Actin Rabbit Monoclonal Antibody 50µl 398.00元
AF5006 β-Actin-HRP Rabbit Monoclonal Antibody 50µl 458.00元
AF5009 GAPDH Mouse Monoclonal Antibody 50µl 398.00元
AF5012 α-Tubulin Rabbit Polyclonal Antibody 50µl 398.00元
AA128 Actin抗体(小鼠单抗) >40次
AA132 Actin抗体(smooth muscle specific,小鼠单抗) >20次
AC610 COX IV抗体(兔多抗) >20次
AF0001 α-Tubulin Rabbit Polyclonal Antibody 100μl
AF0003 β-Actin Mouse Monoclonal Antibody 100μl
AF0006 GAPDH Mouse Monoclonal Antibody 100μl
AF0009 Histone H3 Mouse Monoclonal Antibody 100μl
AF0048 α-Smooth Muscle Actin Rabbit Polyclonal Antibody 50μl
AF1144 Mono/Di-Methyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody 50μl
AF1153 Mono-Methyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody 50μl
AF1165 Mono-Methyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody 50μl
AF1180 Phospho-Histone H3 (Ser10) Rabbit Monoclonal Antibody 50μl
AF1186 GAPDH Rabbit Monoclonal Antibody 50μl
AF1408 Lamin B1 Rabbit Monoclonal Antibody 50μl
AF1684 Acetyl-Histone H3 (Lys56) Rabbit Monoclonal Antibody 50μl
AF1813 Histone H3.3 Rabbit Monoclonal Antibody 50μl
AF2314 Di-Methyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody 50μl
AG019 GAPDH抗体(小鼠单抗) >40次
AH433 Histone H3抗体(兔多抗) >30次
AH436 Di-Methyl-Histone H3(Lys4)抗体(兔单抗) >20次
AH438 Di-Methyl-Histone H3(Lys9)抗体(兔多抗) >20次
AH453 Phospho-Histone H3(Ser10)抗体(小鼠单抗) >20次
AH379 HDAC1抗体(兔多抗) >20次