dNTP,即Deoxy-ribonucleoside triphosphate,中文名为脱氧核糖核苷三磷酸,包括dATP (脱氧腺苷三磷酸)、dCTP (脱氧胞苷三磷酸)、dGTP (脱氧鸟苷三磷酸)、dTTP (脱氧胸苷三磷酸)、dUTP (脱氧尿苷三磷酸)等。其中,dATP、dCTP、dGTP和dTTP作为PCR反应的原料,在PCR体系中不可或缺,在PCR延伸环节中,基于碱基互补配对的原则与模板链结合,在聚合酶的作用下合成新的DNA链,其质量及浓度与PCR反应的扩增效率密切相关。
碧云天生产的dNTP (UltraPure, ≥99.8%)和dNTP (mNGS grade, ≥99.8%)是采用超纯的dATP、dCTP、dGTP、dTTP和dUTP配制的单组分或混合溶液,适合用于需要高纯度dNTP的PCR、real-time PCR、RT-PCR、cDNA或普通DNA合成、引物延伸反应、DNA测序、DNA标记等各种常规分子生物学反应,其中dNTP (mNGS grade, ≥99.8%)专为mNGS测序、高度扩增的高通量测序、LAMP、多重扩增等对dNTP要求较高的PCR反应所设计。
本dNTP (UltraPure, ≥99.8%)系列产品均不含DNase、RNase、缺刻酶(Nickase)和蛋白酶;
本dNTP (mNGS grade, ≥99.8%)系列产品均不含DNase、RNase、Phosphatase、切口酶和蛋白酶,无E.coli DNA、人基因组DNA、mNGS背景核酸污染。
碧云天dNTP系列产品比较与选择
Product | dNTP (UltraPure, ≥99.8%) | dNTP (mNGS grade, ≥99.8%) |
Cat No. | D7420-D7430 | D7450-D7460 |
Purity | ≥99.8% | ≥99.8% |
pH | ~7.0 | ~7.0 |
Thermal Stability | Keep at -20ºC for 2 years, room temperature for 7 days. | Keep at -20ºC for 2 years, room temperature for 7 days. |
Freeze-thaw Stability | 30-50 freeze-thaw cycles | 30-50 freeze-thaw cycles |
DNase Contamination | No | No |
RNase Contamination | No | No |
Nickase Contamination | No | No |
Amplification Detection | Pass | Pass |
Content of Pyrophosphate | NA | Pass |
Phosphatase Activity | NA | Pass |
E.coli DNA Contamination | NA | Pass |
Human Genomic DNA Contamination | NA | Pass |
mNGS Nucleic Acid Background Detection | NA | Pass |
Recommended Application | Conventional reactions such as normal PCR, real-time PCR, RT-PCR, cDNA synthesis, DNA sequencing, DNA Labeling, et al. | Metagenomics NGS, highly-amplified NGS, LAMP, Multiplex PCR, as well as other conventional PCR reactions. |
产品信息: