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pCMV-C-EGFP
Cat. No.:
Pack size: 100μg 1μg
Price: RMB
Purpose
Express target protein with C-terminal EGFP tag in mammalian cell
Depositor
Beyotime
Publication
Sun et al. Autophagy. 2018;14(2):336-346.
Sequence Information
Brief Information
Name Catalog No. Size Description
pCMV-C-EGFP D2626-1μg 5010bp DNA solution
Growth In Bacteria
Bacterial Resistance|Kanamycin
Growth Temperature|37℃
Growth Strain|DH5-Alpha
Copy Number|High Copy
Insert Gene Information
Insert gene|N/A
Species|Synthetic
Insert size (bp)|N/A
Gene Sequences|
Promoter|CMV
T-terminal Tag|N/A
C-terminal Tag|EGFP
Cloning Method|Restriction Enzyme
5'→3' Cloning Site|Sac Ⅰ/XbaⅠ
5′ sequencing primer|T3 5'-AATTAACCCTCACTAAAGGG-3'
3′ sequencing primer | C-EGFP primer 5′-GGGTCAGCTTGCCGTAGGTGGC-3′
Backbone
Vector backbone|pCMV
Vector type | Mammalian expresstion
Total vector size| 5010
 
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