质粒-pCMV-N-CFP

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多肽与蛋白

细胞相关

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pCMV-N-CFP

Cat. No.:D2703-1μg

Pack size: 100μg 1μg

Price: 888.00RMB

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Purpose

Express target protein with N-terminal CFP tag in mammalian cell

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Depositor

Beyotime

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Publication

Sun et al. J Cell Physiol. 2019 Nov;234(11):20623-20633.

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Sequence Information

D2703 pCMV-N-CFP-seq.txt

For Details Add to cart

Brief Information

Name	Catalog No.	Size	Description
pCMV-N-CFP	D2703-1μg	5003bp	DNA solution

Growth In Bacteria

Bacterial Resistance|Kanamycin

Growth Temperature|37℃

Growth Strain|DH5-Alpha

Copy Number|High Copy

Insert Gene Information

Insert gene|N/A

Species|Synthetic

Insert size (bp)|N/A

Gene Sequences|

Promoter|CMV

T-terminal Tag|CFP

C-terminal Tag|N/A

Cloning Method|Restriction Enzyme

5'→3' Cloning Site|BamHⅠ/ApaⅠ

5′ sequencing primer|N-CFP primer 5′-GCACCCAGTCCGCCCTGAGC-3′

3′ sequencing primer | T7 5'-GTAATACGACTCACTATAGGGC- 3'

Backbone

Vector backbone|pCMV

Vector type | Mammalian expresstion

Total vector size| 5003

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