Insert gene|N/A
Species|Synthetic
Insert size (bp)|N/A
Gene Sequences|
Promoter|CMV
T-terminal Tag|EGFP
C-terminal Tag|N/A
Cloning Method|Restriction Enzyme
5'→3' Cloning Site|BamHⅠ/ApaⅠ
5′ sequencing primer|N-EGFP primer 5'-GCACCCAGTCCGCCCTGAGC-3'
3′ sequencing primer
|
T7 5'-GTAATACGACTCACTATAGGGC- 3'