Insert gene|N/A
Species|Synthetic
Insert size (bp)|N/A
Gene Sequences|
Promoter|T7
T-terminal Tag|GST
C-terminal Tag|6X His
Cloning Method|Restriction Enzyme
5'→3' Cloning Site|BamHⅢ/XhoⅠ
5′ sequencing primer|T7 primer
3′ sequencing primer
|
pET-R primer 5′-TGCTAGTTATTGCTCAGCGG-3′