Insert gene|N/A
Species|Synthetic
Insert size (bp)|N/A
Gene Sequences|
Promoter|T7
T-terminal Tag|GST
C-terminal Tag|N/A
Cloning Method|Restriction Enzyme
5'→3' Cloning Site|BamHⅠ/NotⅠ,NdeⅠ/XhoⅠ
5′ sequencing primer|MCS1-N Primer 5′-GCTATCCCACAAATTGATAAGT-3′; MCS2-N Primer 5′-TTGTACACGGCCGCATAATC-3′
3′ sequencing primer
|
MCS1-C Primer 5′-GATTATGCGGCCGTGTACAA-3′