碧云天生物技术-GFP抗体(兔多抗)(AG279)

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GFP抗体(兔多抗)

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产品编号: AG279

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产品编号	产品名称	产品包装	产品价格
AG279	GFP抗体(兔多抗)	>40次	-

来源	用途	抗体识别位点	GFP分子量
Rabbit	WB, IP, IF	Full length GFP	～27kD

      WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence.
      本GFP抗体(GFP antibody)为进口分装，用全长的来源于水母的GFP(green fluorescent protein)免疫rabbit，然后用
protein A和抗原多肽亲和柱经过两步纯化得到的高纯度抗体。
      本GFP抗体可以识别GFP以及GFP的一些突变体例如EGFP(enhanced green fluorescent protein)。
      GFP或其突变体EGFP等被广泛用于基因表达效率的检测，以及和目的蛋白融合表达用于检测目的蛋白的表达和分布。本GFP抗体不仅可以检测GFP或其适当的突变体，也可以检测和GFP或其适当的突变体融合表达的蛋白。
      配套提供了Western一抗稀释液，可以用于Western检测时的一抗稀释。
      建议抗体使用时的稀释比例如下(实际使用时需根据GFP表达水平的高低作适当调整)：

WB	IP	IF
1:200-1000	1:100	1:200

本抗体用于常规的Western检测，如果稀释比例为1:1000，则至少可以检测40次。
包装清单：

产品编号	产品名称	包装
AG279-1	GFP抗体(兔多抗)	40μl
AG279-2	Western一抗稀释液	40ml
－	说明书	1份

保存条件：
      GFP抗体-20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
      对于本抗体，Western检测时一抗要4℃缓慢摇动过夜，如果仅短时间与一抗孵育检测效果较差。
      在Western实验后，请注意回收稀释的抗体。回收的抗体在进行Western实验时至少可以重复使用10次。稀释后的抗体，包括已经使用过的稀释抗体，4℃保存。
      回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
      为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1.Western检测：
A.按照1:1000用碧云天提供的Western一抗稀释液稀释抗体。如果GFP阳性的细胞低于30%，建议适当提高抗体浓度，例如按照1:500或1:200稀释GFP抗体。
B.把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
C.回收稀释的一抗，4℃保存以备下次继续使用。
D.按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2.免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页： http://www.beyotime.com/support/immunol-staining.htm
3.其它实验操作请自行参考适当的protocol进行。

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