碧云天生物技术-Mito-Tracker Red CMXRos (线粒体红色荧光探针,高纯度)(C1035-50μg)

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试剂> 细胞相关> 细胞组织染色> 荧光染色

Mito-Tracker Red CMXRos (线粒体红色荧光探针,高纯度)

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产品编号: C1035-50μg

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产品编号	产品名称	产品包装	产品价格
C1035-50μg	Mito-Tracker Red CMXRos (线粒体红色荧光探针,高纯度)	50μg	402.00元
C1035-250μg	Mito-Tracker Red CMXRos (线粒体红色荧光探针,高纯度)	250μg	1711.00元

Mito-Tracker Red CMXRos (线粒体红色荧光探针)，也称MitoTracker Red CMXRos，是一种具有细胞通透性的X-rosamine衍生物(Chloromethyl-X-rosamine，简称CMXRos)，能够特异性地标记细胞中具有生物活性的线粒体，检测线粒体膜电位。本产品为高纯度高品质探针，兼容后续的细胞固定和通透，但染色后再固定和通透，荧光强度会有一定程度的下降。

本产品是一种氧化型的红色荧光染料，只需简单地和细胞孵育，即可通过被动运输穿过细胞膜，并借助本探针含有的弱巯基反应性的氯甲基(mildly thiol-reactive chloromethyl)官能团特异性地标记有生物活性的线粒体。本探针含有的弱巯基反应性的氯甲基，可以和线粒体内蛋白的巯基反应并共价连接，因此后续实验使用多聚甲醛或甲醛等醛类固定剂，以及细胞通透的去垢剂Triton X-100等处理时，线粒体的荧光标记不会消失，但可能会有一定程度的下降(约下降2-10倍)。由于本荧光探针的激发光谱和发射光谱与常见的绿色荧光探针重叠较少，因此非常适合用于荧光双标实验。

Mito-Tracker Red CMXRos分子式为C₃₂H₃₂Cl₂N₂O，分子量为531.5，CAS number为167095-09-2，Mito-Tracker Red CMXRos呈红色荧光，最大激发波长为579nm，最大发射波长为599nm。Mito-Tracker Red CMXRos的激发光谱和发射光谱以及结构式参考图1。

图1.Mito-Tracker Red CMXRos的激发光谱和发射光谱(A)以及结构式(B)。

Mito-Tracker Red CMXRos可以用作线粒体特异性的荧光探针。和Rhodamine 123或JC-1相似，Mito-Tracker Red CMXRos对于线粒体的染色依赖于线粒体膜电位，因此该探针只能对活的细胞或组织进行染色，不能对固定或通透后的细胞或组织进行染色。使用Mito-Tracker Red CMXRos染色活细胞线粒体效果参考图2。

图2.Mito-Tracker Red CMXRos (高纯度)染色NRK-52E细胞(大鼠肾小管上皮细胞)线粒体的效果。图A为明场下大鼠肾小管上皮细胞形态图；蓝色荧光为Hoechst 33342染色(C1027/C1028/C1029 Hoechst 33342活细胞染色液(100X))大鼠肾小管上皮细胞的细胞核(图B)，红色荧光为Mito-Tracker Red CMXRos染色大鼠肾小管上皮细胞的线粒体(图C)，图D为Hoechst 33342和Mito-Tracker Red CMXRos双染的大鼠肾小管上皮细胞的细胞核与线粒体。实测数据会因实验条件、检测仪器等的不同而存在差异，图中数据仅供参考。

由于Mito-Tracker Red CMXRos在线粒体内的聚集依赖于线粒体的膜电位，因此本产品也可以作为线粒体膜电位的指示探针，通过检测线粒体膜电位的变化来检测细胞凋亡。

按最终工作浓度为50-200nM计算，本产品每50µg可以配制约500-2000ml Mito-Tracker Red CMXRos工作液。

包装清单：

产品编号	产品名称	包装
C1035-50µg	Mito-Tracker Red CMXRos (高纯度)	50µg
C1035-250µg	Mito-Tracker Red CMXRos (高纯度)	50µg×5
—	说明书	1份

保存条件：

-20℃避光保存，一年有效。

注意事项：

Mito-Tracker Red CMXRos (高纯度)可以用于活细胞的线粒体荧光染色，不适合用于固定后细胞或者组织的染色；但在用本产品标记线粒体后，后续可以进行固定和通透，并用其它探针进行染色或进行免疫荧光染色，但染色后再固定和通透，荧光强度会有一定的下降。而且不同的细胞，固定和通透的影响会有所区别，建议对固定和通透试剂、条件进行一定的测试。

本产品仅包含非常微量的粉末，使用前请先离心数秒钟，使微量粉末充分沉降到管底。

荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。

使用本产品可以在常见的多孔板或培养皿中进行荧光染色，也可以在盖玻片或载玻片上进行荧光染色。

本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。

为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1.Mito-Tracker Red CMXRos储存液的配制：
取一管50μg Mito-Tracker Red CMXRos粉末加入470μl的无水DMSO (anhydrous dimethylsulfoxide)，充分溶解后，得到浓度为200μM的Mito-Tracker Red CMXRos储存液。也可以根据需要配制成其它浓度的储存液，如1mM。适当分装后避光保存于-20℃或更低温度。
2.Mito-Tracker Red CMXRos工作液的配制：
a.取少量200μM Mito-Tracker Red CMXRos储存液按照1:1000-1:10,000的比例加入到细胞培养液或适当的溶液(例如含钙镁离子的HBSS)中，使最终浓度为20nM-200nM。例如取1µl 200μM的Mito-Tracker Red CMXRos储存液加入到1ml细胞培养液或适当的溶液(例如含钙镁离子的HBSS)中，混匀后即为200nM的Mito-Tracker Red CMXRos工作液。如果染色的细胞后续需要进行固定或通透等步骤，建议工作浓度为50nM-200nM。Hanks' Balanced Salt Solution (with Ca²⁺ & Mg²⁺) (C0219)可以向碧云天订购。
b.Mito-Tracker Red CMXRos工作液使用前需37℃预温育。
注：Mito-Tracker Red CMXRos工作液的浓度可以根据实际情况进行适当调整。为降低非特异性荧光染色和线粒体毒性，在染色效果可以接受的范围内，建议尽量使用较低浓度的Mito-Tracker Red CMXRos。
3.贴壁细胞的线粒体染色：
a.当细胞在细胞培养板或培养皿中培养至一定密度时，去除细胞培养液，加入步骤2中配制好的Mito-Tracker Red CMXRos工作液，37℃孵育15-30分钟。注：最佳孵育时间需根据细胞类型进行适当的优化。
b.去除Mito-Tracker Red CMXRos工作液，加入37℃预温育的新鲜细胞培养液。
c.用荧光显微镜、激光共聚焦显微镜或荧光酶标仪进行观察或检测。此时可观察到线粒体呈明亮的强荧光染色。如果染色效果欠佳，可以提高Mito-Tracker Red CMXRos工作液浓度或在推荐的时间范围内适当延长染色时间。
注：标记的Mito-Tracker Red CMXRos易淬灭，请注意尽快进行拍照等荧光检测。也可以通过降低荧光显微镜的激发光强度(即汞灯或LED光源)或适当降低Mito-Tracker Red CMXRos工作液浓度以延缓淬灭。
4.悬浮细胞的线粒体染色：
a.1000×g离心5分钟，弃上清，用37℃预热的Mito-Tracker Red CMXRos工作液轻轻重悬细胞，37℃孵育15-30分钟。注：最佳孵育时间需根据细胞类型进行适当的优化。
b.孵育结束后，1000g离心5分钟，弃上清，加入37℃预温育的新鲜细胞培养液重悬细胞。
c.用荧光显微镜、激光共聚焦显微镜、流式细胞仪或荧光酶标仪进行观察、分析或检测。
注：如果需要在载玻片或盖玻片上固定悬浮细胞，可先用碧云天的Poly-D-lysine/多聚赖氨酸(ST508)处理载玻片或盖玻片。
5.染色后的细胞固定或细胞通透(选做)：
用本试剂染色线粒体后，可进行固定和通透，并用其它探针再进行染色。
a.细胞固定：在染色后，用37℃温育的新鲜细胞培养液或HBSS洗涤细胞，小心吸除洗涤用的培养液，加入4%多聚甲醛固定液(例如P0099)或含3.7%新鲜甲醛的完全培养液，室温固定15-30分钟。固定完成后用HBSS洗涤2-3次。
注：采用37℃预热的固定液并在37℃固定约15min，可更好地维持细胞形态。对于内皮细胞，在本染料染色之后再用含3.7%甲醛的完全培养液于37℃孵育15分钟有良好的固定效果。
b.细胞通透：将固定后的细胞加入含约0.2% Triton X-100的PBS或碧云天生产的免疫染色通透液(Triton X-100) (P0096)或免疫染色强力通透液(P0095)，室温孵育10-15分钟。然后用PBS洗涤。使用本探针染色活细胞后，再固定和通透的细胞内荧光效果参考图3。
注1：对于内皮细胞，在本染料染色之后再用含约0.2% Triton X-100的PBS室温孵育10分钟有良好的通透效果。
注2：也可用丙酮冰浴通透5分钟，然后用PBS洗涤。即使后续无需抗体标记，丙酮通透处理对降低背景信号也会有一定的帮助。
注3：染色后再固定和通透，荧光强度会有一定的下降，而且不同的细胞，固定和通透的影响会有所区别，建议对固定和通透试剂的浓度等进行一定的摸索。

图3.Mito-Tracker Red CMXRos (高纯度)染色NRK-52E细胞后再经固定和通透的细胞荧光染色效果。正常NRK-52E细胞Mito-Tracker Red CMXRos (高纯度) 染色后线粒体内呈清晰明亮的红色荧光(图A)；染色后经4%多聚甲醛固定液(P0099)室温固定15分后细胞内红色荧光略有下降，荧光出现一定程度的弥散(图B)；固定后经免疫染色强力通透液(P0095)通透后细胞内红色荧光进一步下降(约下降2-10倍)，细胞内线粒体呈较弱的红色荧光，但线粒体染色相对比较清晰(图C)。注：由于通透后线粒体荧光有一定程度的下降，为了方面观察，图C延长了拍摄时的曝光时间。实测数据会因实验条件、检测仪器等的不同而存在差异，图中数据仅供参考。
相关产品：

产品编号	产品名称	包装
P0106	免疫染色洗涤液	250ml
C0219	Hanks' Balanced Salt Solution (with Ca2+ & Mg2+)	500ml
C1035	Mito-Tracker Red CMXRos (线粒体红色荧光探针,高纯度)	50μg/250μg
C1036	DiI (细胞膜红色荧光探针)	10mg
C1038	DiO (细胞膜绿色荧光探针)	10mg
C1041	ER-Tracker Red (内质网红色荧光探针)	20μl
C1043	Golgi-Tracker Red (高尔基体红色荧光探针)	1mg
C1046	Lyso-Tracker Red (溶酶体红色荧光探针)	50μl
C1048	Mito-Tracker Green (线粒体绿色荧光探针)	50μg
C1049B	Mito-Tracker Red CMXRos (线粒体红色荧光探针)	50µg/250µg
C1050	Tubulin-Tracker Red (微管红色荧光探针)	40μl
C1062	Annexin V-FITC细胞凋亡检测试剂盒	20次
C1071	线粒体膜电位与细胞凋亡检测试剂盒	20次/50次
C2006	线粒体膜电位检测试剂盒(JC-1)	>100次
C2007	Rhodamine 123	5mg
C2201S	Actin-Tracker Green-488 (微丝绿色荧光探针)	0.2ml

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22. Xiao-Long Li, Xue-Wu Liu, Wei-Ling Liu, Yu-Quan Lin, Jing Liu, Yu-Sheng Peng, Li-Min Cheng, Yan-Hua Du

Inhibition of TMEM16A improves cisplatin-induced acute kidney injury via preventing DRP1-mediated mitochondrial fission

Acta Pharmacol Sin. 2023 Nov;44(11):2230-2242. doi: 10.1038/s41401-023-01122-6.

23. Shifeng Zhang, Zhezhi Deng, Yuemin Qiu, Gengxin Lu, Junyu Wu, Haiwei Huang

FGIN-1-27 Mitigates Radiation-induced Mitochondrial Hyperfunction and Cellular Hyperactivation in Cultured Astrocytes

Neuroscience. 2023 Dec 15:535:23-35. doi: 10.1016/j.neuroscience.2023.10.017.

24. Quanwei Li, Shaofeng Wang, Pan Guo, Yuanhong Feng, Wenlan Yu, Hui Zhang, Jianying Guo, Ying Li, Lianmei Hu, Jiaqiang Pan, Jianzhao Liao, Zhaoxin Tang

Mitochondrial DNA release mediated by TFAM deficiency promotes copper-induced mitochondrial innate immune response via cGAS-STING signalling in chicken hepatocytes

Sci Total Environ. 2023 Dec 20:905:167315. doi: 10.1016/j.scitotenv.2023.167315.

25. Yannan Li, Junnan Li, Lixuan Yang, Feifei Ren, Kaiqiang Dong, Zhonghui Zhao, Wenzhe Duan, Wei Wei, Rongjuan Guo

Ginsenoside Rb1 protects hippocampal neurons in depressed rats based on mitophagy-regulated astrocytic pyroptosis

Phytomedicine. 2023 Dec:121:155083. doi: 10.1016/j.phymed.2023.155083.

26. Xiaoyu Tang, Zhijie Huang, Fengjiao Wang, Jin Chen, Donglu Qin, Daoquan Peng, Bilian Yu

Macrophage-specific deletion of MIC26 (APOO) mitigates advanced atherosclerosis by increasing efferocytosis

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27. Junchen Guo, Shanjie Wang, Xin Wan, Xiaoxuan Liu, Zeng Wang, Chenchen Liang, Zhenming Zhang, Ye Wang, Miao Yan, Pengyan Wu, Shaohong Fang, Bo Yu

Mitochondria-derived methylmalonic acid aggravates ischemia-reperfusion injury by activating reactive oxygen species-dependent ferroptosis

Cell Commun Signal. 2024 Jan 18;22(1):53. doi: 10.1186/s12964-024-01479-z.

28. Guoqing Zhao, Te Zhang, Jiannan Li, Longyun Li, Peng Chen, Chunlu Zhang, Kai Li, Cancan Cui

Parkin-mediated mitophagy is a potential treatment for oxaliplatin-induced peripheral neuropathy

Am J Physiol Cell Physiol. 2024 Jan 1;326(1):C214-C228. doi: 10.1152/ajpcell.00276.2023.

29. Tianqing Yan, Xiaolu Ma, Kaixia Zhou, Jiazhen Cao, Yanan Tian, Hui Zheng, Ying Tong, Suhong Xie, Yanchun Wang, Lin Guo, Renquan Lu

A novel CSN5/CRT O-GlcNAc/ER stress regulatory axis in platinum resistance of epithelial ovarian cancer

Int J Biol Sci. 2024 Jan 27;20(4):1279-1296. doi: 10.7150/ijbs.89700.

30. Wan Xie, Huimin Shi, Rankun Zuo, Shendong Zhou, Nana Ma, Hongzhu Zhang, Guangjun Chang, Xiangzhen Shen

Conjugated Linoleic Acid Ameliorates Hydrogen Peroxide-Induced Mitophagy and Inflammation via the DRP1-mtDNA-STING Pathway in Bovine Hepatocytes

J Agric Food Chem. 2024 Jan 31;72(4):2120-2134. doi: 10.1021/acs.jafc.3c02755.

31. Kang Xia, Jiayu Guo, Bo Yu, Tianyu Wang, Qiangmin Qiu, Qi Chen, Tao Qiu, Jiangqiao Zhou, Shusen Zheng

Sentrin-specific protease 1 maintains mitochondrial homeostasis through targeting the deSUMOylation of sirtuin-3 to alleviate oxidative damage induced by hepatic ischemia/reperfusion

Free Radic Biol Med. 2024 Jan:210:378-389. doi: 10.1016/j.freeradbiomed.2023.11.040.

32. Xiaozhe Su, Chao Song, Ziqi He, Qianlin Song, Lingchao Meng, Caitao Dong, Jiawei Zhou, Hu Ke, Yunhe Xiong, Junwei Liu, Wenbiao Liao, Sixing Yang

Ambra1 in exosomes secreted by HK-2 cells damaged by supersaturated oxalate induce mitophagy and autophagy-ferroptosis in normal HK-2 cells to participate in the occurrence of kidney stones

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33. Yuping Zeng, Yijun Lin, Juhua Yang, Xiaohui Wang, Yihua Zhu, Biting Zhou

The Role and Mechanism of Nicotinamide Riboside in Oxidative Damage and a Fibrosis Model of Trabecular Meshwork Cells

Transl Vis Sci Technol. 2024 Mar 1;13(3):24. doi: 10.1167/tvst.13.3.24.

34. Fan Chen, An-Feng Luo, Ming-Guo Li, Li-Xiang Zheng, Hao Gu, Chang-Fan Zhou, Wei Zeng, Adrian Molenaar, Hong-Yan Ren, Yan-Zhen Bi

3-Methyl-4-nitrophenol Exposure Deteriorates Oocyte Maturation by Inducing Spindle Instability and Mitochondrial Dysfunction

Int J Mol Sci. 2024 Mar 22;25(7):3572. doi: 10.3390/ijms25073572.

35. Yang Yang, Yue Tao, Rongyi Yang, Xiaodong Yi, Guanyu Zhong, Yanyan Gu, Ying Zhang

Ca2+ homeostasis imbalance induced by Pparg: A key factor in di (2-ethylhexyl) phthalate (DEHP)-induced cardiac dysfunction in zebrafish larvae

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36. Qiwei He, Tiantian Yu, Junxiong Chen, Jianli Liang, Dongni Lin, Kaihao Yan, Zijing Xie, Yuqi Song, Zhenzhou Chen

Enhancement of de novo lipogenesis by the IDH1 and IDH2-dependent reverse TCA cycle maintains the growth and angiogenic capacity of bone marrow-derived endothelial progenitor cells under hypoxia

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37. Lanhe Chu, Jinzhong Zhuo, Haohua Huang, Weimou Chen, Wenshan Zhong, Jinming Zhang, Xiaojing Meng, Fei Zou, Shaoxi Cai, Mengchen Zou, Hangming Dong

Tetrandrine alleviates pulmonary fibrosis by inhibiting alveolar epithelial cell senescence through PINK1/Parkin-mediated mitophagy

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38. Yue Tao, Xiaodong Yi, Yanyan Gu, Rongyi Yang, Zixu Li, Xiangyong Guo, Donglin Zhao, Ying Zhang

Neurotoxicity of dibutyl phthalate in zebrafish larvae: Decreased energy acquisition by neurons

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39. Cong Ma, Hongzhen Ruan, Huiru Cheng, Zuying Xu, Caiyun Wu, Dan Liang, Huifen Xiang, Yunxia Cao, Zhiming Ding

Triphenyltin chloride exposure inhibits meiotic maturation of mouse oocytes by disrupting cytoskeleton assembly and cell cycle progression

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40. Yingying Sun, Le Xu, Yi Li, Shunze Jia, Gang Wang, Xufeng Cen, Yuyan Xu, Zhongkai Cao, Jingjing Wang, Ning Shen, Lidan Hu, Jin Zhang, Jianhua Mao, Hongguang Xia, Zhihong Liu, Xudong Fu

Mitophagy defect mediates the aging-associated hallmarks in Hutchinson-Gilford progeria syndrome

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41. Huabin Wang, Yi Li, Xichao Cao, Heping Niu, Xiaoran Li, Jirong Wang, Jianwei Yang, Changhong Xu, Hailong Wang, Shun Wan, Kunpeng Li, Shengjun Fu, Li Yang

MELATONIN ATTENUATES RENAL ISCHEMIA-REPERFUSION INJURY BY REGULATING MITOCHONDRIAL DYNAMICS AND AUTOPHAGY THROUGH AMPK/DRP1

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42. Zhengyue Chen, Caiming Chen, Kunmei Lai, Chengkun Wu, Fan Wu, Zhimin Chen, Keng Ye, Jingzhi Xie, Huabin Ma, Hong Chen, Yujia Wang, Yanfang Xu

GSDMD and GSDME synergy in the transition of acute kidney injury to chronic kidney disease

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43. Qingsheng Li, Lingfei Yang, Kaixin Wang, Ziyi Chen, Huimin Liu, Xuan Yang, Yudi Xu, Yufei Chen, Zhe Gong, Yanjie Jia

Oxidized mitochondrial DNA activates the cGAS-STING pathway in the neuronal intrinsic immune system after brain ischemia-reperfusion injury

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44. Mingfang Xiong, Yanyu Xiu, Juan Long, Xiao Zhao, Qianqian Wang, Haoyu Yang, Hang Yu, Lihong Bian, Yan Ju, Hongyu Yin, Qingxiang Hou, Fei Liang, Nan Liu, Fudong Chen, Ruiwen Fan, Yuying Sun, Yang Zeng

Proteomics reveals dynamic metabolic changes in human hematopoietic stem progenitor cells from fetal to adulthood

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45. Xiu-Li Zhang, Zhi-Yong An, Gao-Jie Lu, Tuo Zhang, Cheng-Wei Liu, Meng-Qi Liu, Qing-Xin Wei, Lin-Hu Quan, Jin-Dan Kang

MCT1-mediated transport of valeric acid promotes porcine preimplantation embryo development by improving mitochondrial function and inhibiting the autophagic AMPK-ULK1 pathway

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46. Weijia Feng, Nannan Chen, Ke Chen, Ting Chen

Forkhead box O-1 regulates the biological behavior of BMP-2-induced human bone mesenchymal stem cells through mitochondrial dynamics and autophagy

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47. Enguang Yang, Suoshi Jing, Fang Wang, Hanzhang Wang, Shengjun Fu, Li Yang, Junqiang Tian, Dragan J Golijanin, Wafik S El-Deiry, Liang Cheng, Zhiping Wang

Mesenchymal stem cells in tumor microenvironment: drivers of bladder cancer progression through mitochondrial dynamics and energy production

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48. Yujia Song, Xiaohui Wang, Yuqi Sun, Nianhua Yu, Yajie Tian, Jinli Han, Xianjun Qu, Xinfeng Yu

PRDX1 inhibits ferroptosis by binding to Cullin-3 as a molecular chaperone in colorectal cancer

Int J Biol Sci. 2024 Sep 23;20(13):5070-5086. doi: 10.7150/ijbs.99804.

49. Yi Zhang, Chen Zhang, Rui Feng, Tong Meng, Wei Peng, Jian Song, Wenming Ma, Wenlong Xu, Xianguo Chen, Jing Chen, Chaozhao Liang

CXCR4 regulates macrophage M1 polarization by altering glycolysis to promote prostate fibrosis

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50. Shuyun Wu, Huiling Liu, Jiazhi Yi, Minyi Xu, Jie Jiang, Jin Tao, Bin Wu

β-arrestin1 protects intestinal tight junction through promoting mitofusin 2 transcription to drive parkin-dependent mitophagy in colitis

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51. MengDa Xu, Jie Zhu, Zhen Wang, JingYu Yan, Xiang Zhou

Neuroprotective effect of autologous mitochondrial transplantation against global ischemia/reperfusion injury in a rat model of cardiac arrest

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52. Lin Cui, Xiaohui Xu, Hui Fan, Xinyan Wan, Qian Chen, Junhui Zhang, Chuntao Tao, Zheng Du, Yitao Wang, Jian Zhang, Jun Zeng, Ying Zhang, Chundong Zhang, Longhao Li, Youquan Bu, Yunlong Lei

Reuterin promotes pyroptosis in hepatocellular cancer cells through mtDNA-mediated STING activation and caspase 8 expression

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53. Jiemei Shi, Min Liu, Jiajie Zhao, Ye Tan, Chunhui Jiang

Honokiol protects against diabetic retinal microvascular injury via sirtuin 3-mediated mitochondrial fusion

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54. Liang Zhao, Changping Gu, Yi Zhang, Jie Yan, Lei Qiu, Xiaofeng Qin, Yuelan Wang

Regulation mechanism of GPS2 on PGC-1α/Drp1-mediated mitochondrial dynamics in inflammation of acute lung injury

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55. Leijing Yin, Ludong Yuan, Zhengyang Luo, Yuting Tang, Xiaofang Lin, Shuxin Wang, Pengfei Liang, Lingjin Huang, Bimei Jiang

COX-2 optimizes cardiac mitochondrial biogenesis and exerts a cardioprotective effect during sepsis

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56. You-Qin Yin, Le-le Liu, Yu-Ting Jiang, Jin-Chao Xing, Wen-Bao Qi, Li-Hong Huang

SLC25A12 inhibits Japanese encephalitis virus replication by interacting with the NS1 and enhancing the type I interferon response

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57. Lin Wang, Yao Wang, Mengyue Wu, Xing Jin, Yifei Chen, Zhenhuan Guo, Xiaowen Meng, Jianyou Zhang, Fuhai Ji

Minocycline alleviates microglia ferroptosis by inhibiting HO-1 during cerebral ischemia-reperfusion injury

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58. Zhiming Zhang, Binwei Jin, Yang Zhang, Minjun Yang, Cheng Wang, Yu Zhu, Tao Li, Jiangbo Lin, Mengqi Yang, Ying Cheng, Shasha Xu, Kui He, Jiayi Xu, Yafei Mi, Jianjun Jiang, Zhenzhu Sun

USP14 modulates cell pyroptosis and ameliorates doxorubicin-induced cardiotoxicity by deubiquitinating and stabilizing SIRT3

Free Radic Biol Med. 2024 Nov 20:225:741-757. doi: 10.1016/j.freeradbiomed.2024.10.302.

59. Yaxi Shang, Ziran Wang, Fan Yang, Weidong Wang, Qingzhu Tang, Xianan Guo, Xiangning Du, Xu Zhang, Jiaojiao Hao, Hongli Lin

FUT8 upregulates CD36 and its core fucosylation to accelerate pericyte-myofibroblast transition through the mitochondrial-dependent apoptosis pathway during AKI-CKD

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60. Haobin Zhou, Xiao Wang, Tianyu Xu, Daojing Gan, Zhuang Ma, Hao Zhang, Jian Zhang, Qingchun Zeng, Dingli Xu

PINK1-mediated mitophagy attenuates pathological cardiac hypertrophy by suppressing the mtDNA release-activated cGAS-STING pathway

Cardiovasc Res. 2024 Nov 5:cvae238. doi: 10.1093/cvr/cvae238.

61. Kang Xia, Zeya Jin, Qiangmin Qiu, Yujie Zhou, Yifan Lu, Tao Qiu, Jiangqiao Zhou, Zhongbao Chen

Ligustilide alleviates oxidative stress during renal ischemia-reperfusion injury through maintaining Sirt3-dependent mitochondrial homeostasis

Phytomedicine. 2024 Nov:134:155975. doi: 10.1016/j.phymed.2024.155975.

62. Hang Pan, Qian Su, Panjing Hong, Yanli You, Limeng Zhou, Junbo Zou, Jingping Sun, Gaolong Zhong, Jianzhao Liao, Hui Zhang, Zhaoxin Tang, Lianmei Hu

Arsenic-induced mtDNA release promotes inflammatory responses through cGAS-STING signaling in chicken hepatocytes

Pestic Biochem Physiol. 2024 Nov:205:106129. doi: 10.1016/j.pestbp.2024.106129.

63. Changbin Chu, Shangjing Liu, Zhiting He, Mingjun Wu, Jing Xia, Hongxiang Zeng, Wenhua Xie, Rui Cheng, Xueya Zhao, Xi Li

HADH suppresses clear cell renal cell carcinoma progression through reduced NRF2-dependent glutathione synthesis

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64. Yijie Song, Mengjie Zhu, Md Ariful Islam, Wenyi Gu, Kavsar Alim, Chien-Shan Cheng, Jingxian Chen, Yu Xu, Hongxi Xu

Glutathione peroxidase 3 is essential for countering senescence in adipose remodelling by maintaining mitochondrial homeostasis

Redox Biol. 2024 Nov:77:103365. doi: 10.1016/j.redox.2024.103365.

65. Weibo Huang, Feng Hua, Tong Su, Chenghao Zhou, Kangcheng Zhao, Dianwen Song

sEV-mediated lipid droplets transferred from bone marrow adipocytes promote ferroptosis and impair osteoblast function

J Lipid Res. 2024 Nov;65(11):100657. doi: 10.1016/j.jlr.2024.100657.

66. Zhi-Yong An, Sheng-Zhong Han, Zhou-Yan Li, Shuang-Yan Chang, Xiu-Li Zhang, Gao-Jie Lu, Tuo Zhang, Biao-Hu Quan, Xi-Jun Yin, Lin-Hu Quan, Jin-Dan Kang

Eicosatrienoic acid enhances the quality of in vitro matured porcine oocytes by reducing PRKN-mediated ubiquitination of CISD2

Theriogenology. 2024 Dec:230:285-298. doi: 10.1016/j.theriogenology.2024.09.020.

67. Yu Zhou, Wenhuo Xie, Chenghua Kong, Wei Luo, Hong Wei, Jiaping Zheng

Regulatory roles of histamine receptor in astrocytic glutamate clearance under conditions of increased glucose variability

Biochem Pharmacol. 2024 Dec;230(Pt 3):116611. doi: 10.1016/j.bcp.2024.116611.

68. Jiemei Shi, Min Liu, Haohao Zhu, Chunhui Jiang

SIRT3 mitigates high glucose-induced damage in retinal microvascular endothelial cells via OPA1-mediated mitochondrial dynamics

Exp Cell Res. 2025 Jan 15;444(2):114320. doi: 10.1016/j.yexcr.2024.114320.

69. Yuxi Yang, Yiqiu Peng, Yingying Li, Tingjuan Shi, Ning Xu, Yingyi Luan, Chenghong Yin

Sestrin2 balances mitophagy and apoptosis through the PINK1-Parkin pathway to attenuate severe acute pancreatitis

Cell Signal. 2025 Feb:126:111518. doi: 10.1016/j.cellsig.2024.111518.

70. Yajie Chen, Shuang Zhang, Yifan Sun, Jialun Zou, Xuan Qiu, Haotong Xi, Yongnan Xu, Yinghua Li, Bangzhu Chen, Jianglin Fan, Maobi Zhu