碧云天生物技术-Golgi-Tracker Red (高尔基体红色荧光探针)(C1043)

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Golgi-Tracker Red (高尔基体红色荧光探针)

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产品编号: C1043

产品包装:1mg

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价格: ¥ 1689.00

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产品编号	产品名称	产品包装	产品价格
C1043	Golgi-Tracker Red (高尔基体红色荧光探针)	1mg	1689.00元

Golgi-Tracker Red是一种高尔基体红色荧光探针，是神经鞘脂(sphingolipid)类荧光探针中的一种，可以用于活细胞高尔基体特异性荧光染色。
Golgi-Tracker Red为采用Molecular Probes公司的BODIPY TR进行了荧光标记的C5-ceramide。Ceramide或其类似物可以选择性地和高尔基体结合，因此荧光标记的ceramide可以用作高尔基体特异性的荧光探针。Golgi-Tracker Red可以用于活细胞的高尔基体荧光标记，但不适合用于固定细胞的标记。Golgi-Tracker Red的化学结构式参考图1。
图1.Golgi-Tracker Red的化学结构式。 Golgi-Tracker Red分子式为C₃₉H₅₀BF₂N₃O₄S，分子量为705.71，呈红色荧光，最大激发波长为589nm，最大发射波长为617nm。Golgi-Tracker Red的激发光谱和发射光谱参考图2。
图2. Golgi-Tracker Red的激发光谱和发射光谱。 Golgi-Tracker Red常用于活细胞的脂类运输和代谢研究，相比较于传统的同类型探针NBD C₆-ceramide，其呈现出更高的摩尔吸光系数和光量子产量，且光稳定性更强。除了以上应用，本探针还可用来测定Schwann细胞内脂类合成的速率，以及标记细胞轮廓以便于共聚焦显微镜下观察形态运动。
本Golgi-Tracker Red探针已与BSA形成复合物。本产品所属的神经鞘脂类荧光探针与BSA形成复合物后，对活细胞高尔基体的标记会更加高效。
本试剂盒提供了Golgi-Tracker Red稀释液，使Golgi-Tracker Red的使用更加便捷。
按照1:100的比例稀释，可以配制3ml Golgi-Tracker Red工作液；按照1:200的比例稀释，可以配制6ml Golgi-Tracker Red工作液。
包装清单：

产品编号	产品名称	包装
C1043-1	Golgi-Tracker Red (33.3mg/ml, 30µl)	1mg
C1043-2	Golgi-Tracker Red稀释液	6ml
－	说明书	1份

保存条件：
-20℃保存，半年有效。Golgi-Tracker Red需-20℃避光保存。
注意事项：
对于微量的液体，每次使用前先离心数秒钟，使液体充分沉降到管底。
荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。
需自备盖玻片和载玻片(可以向碧云天订购)。
Golgi-Tracker Red可以用于活细胞的高尔基体荧光标记，但不适合用于固定细胞高尔基体的荧光标记。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1.Golgi-Tracker Red工作液的配制：
a.取少量Golgi-Tracker Red按照1:100的比例加入到Golgi-Tracker Red稀释液中。例如取10µl Golgi-Tracker Red加入到1ml Golgi-Tracker Red稀释液中。混匀后即为Golgi-Tracker Red工作液。
注：工作液中Golgi-Tracker Red的浓度可以根据实际情况进行适当调整，推荐的稀释比例调整范围为1:50-1:200。
b.Golgi-Tracker Red工作液可以在首次使用后回收保存于4℃或-20℃，并重复使用。1-2天内可以4℃保存，更长时间则需-20℃保存。使用至达不到预期效果时可以废弃。
2.活细胞高尔基体的荧光标记：
a.去除细胞培养液，用适量的溶液如HBSS with Ca²⁺ & Mg²⁺ (Hanks' Balanced Salt Solution with Ca²⁺ & Mg²⁺)洗涤生长在盖玻片上的细胞。注：HBSS with Ca²⁺ & Mg²⁺ (C0219)可以向碧云天订购；对于悬浮细胞的染色可以参考贴壁细胞的染色方法进行。
b.去除洗涤液，加入步骤1配制好的Golgi-Tracker Red染色工作液，与细胞4℃共孵育30分钟。
c.回收Golgi-Tracker Red染色工作液，用冰浴预冷的细胞培养液洗涤细胞3次左右，换新鲜培养液37℃孵育30分钟。
d.用新鲜培养液再洗涤一次，随后通常用荧光显微镜或激光共聚焦显微镜进行观察。此时可观察到高尔基体呈明亮的强荧光染色，而细胞内的其他膜系统呈比较微弱的荧光染色。

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