产品编号 | 产品名称 | 产品包装 | 产品价格 |
C6669 | NCI-H23 (人非小细胞肺癌细胞) | 1支/瓶 | 1168.00元 |
Organism | Tissue | Morphology | Culture Properties |
Homo sapiens (Human) | lung | epithelial | adherent |
本细胞株详细信息如下:
General Information | |
Cell Line Name | NCI-H23 (Human Non-Small Cell Lung Cancer Cells) |
Synonyms | NCI.H23; NCI H23; H23; H-23; NCIH23 |
Organism | Homo sapiens (Human) |
Tissue | lung |
Cell Type | - |
Morphology | epithelial |
Disease | adenocarcinoma; non-small cell lung cancer |
Strain | - |
Biosafety Level* | 1 |
Age at Sampling | 51 years |
Gender | male |
Genetics | - |
Ethnicity | Black |
Applications |
3D cell culture Cancer research High-throughput screening Toxicology |
Category | Cancer cell line |
* Biosafety classification is based onU.S. Public Health Service Guidelines,it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Characteristics | |
Karyotype | - |
Virus Susceptibility | - |
Derivation | This line was derived from a lung cancer obtained from a patient prior to therapy. |
Clinical Data |
51 years Black male This line was derived from a lung cancer obtained from a patient prior to therapy. |
Antigen Expression | - |
Receptor Expression | - |
Oncogene | myc +; src +; abl +; erb +; ras +; sis - |
Genes Expressed | myc +; src +; abl +; erb +; ras +; sis - |
Gene expression databases | - |
Metastasis | - |
Tumorigenic | - |
Effects | - |
Comments |
The cells carry the K-ras 12 mutation, and there is a mutation in codon 246 (ATC -> ATG, isoleucine -> methionine) of the p53 gene. There is expression of C-myc, L-myc, v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras and N-ras RNAs. The cells express heterogeneous mRNA expression for PDGF A and B chain, transforming growth factor alpha and beta and the epidermal growth factor receptor (EGFR). NCI-H23 exhibits a high degree of c-myc DNA amplification (20-fold) but no detectable amplification of c-myc RNA. The cells stain positive for keratins 5+8 and 18 and vimentin but are negative for neurofilament. NCI-H23 cells are L-dopa decarboxylase-negative. They have a reported colony forming efficiency of 9.7% in soft agarose. |
Culture Method | |
Doubling Time | - |
Methods for Passages | Remove spent medium, and add fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes at room temperature. Remove the trypsin and incubate the flask at 37°C for 5 to 10 minutes or until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
Medium | RPMI 1640+10% FBS |
Special Remarks | - |
Medium Renewal | Every 2 to 3 days |
Subcultivation Ratio | 1:3 to 1:6 |
Growth Condition | 95% air + 5% CO2, 37ºC |
Freeze medium | RPMI 1640+20% FBS+10% DMSO,也可以订购碧云天的细胞冻存液(C0210)。 |
本细胞株经过支原体检测(Mycoplasma Test),检测结果为阴性。
本细胞株通过STR (short tandem repeats)鉴定,具体结果如下:
D5S818 | D13S317 | D7S820 |
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D16S539 | vWA | THO1 |
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AM | TPOX | CSF1PO |
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本细胞株的培养图片如下:
产品编号 | 产品名称 | 包装 |
C6669 | NCI-H23 (人非小细胞肺癌细胞) | 1支/瓶 |
— | 说明书 | 1份 |
对于细胞培养瓶或离心管运输的活细胞,室温3-5天有效。对于干冰运输的冻存细胞,液氮保存,长期有效;-80℃保存,2个月有效。
注意事项:本细胞株未经碧云天书面许可不得用于任何商业用途,也不得移交给订货人所在实验室外的任何个人或单位。使用者在发表研究论文或结果时,应注明细胞株的来源。
本细胞株相关资料参考ATCC (American Type Culture Collection)、DSMZ (German Collection of Microorganisms and Cell Cultures)、JCRB (Japanese Collection of Research Bioresources Cell Bank)、Cellosaurus (Swiss Institute of Bioinformatics)等网站信息,并结合碧云天实际培养信息综合而成。由于细胞培养的条件、代数等因素,实际细胞可能与本说明书提供的信息有一定的差异,具体以实际细胞为准。
STR结果可以与ATCC、DSMZ及中国国家实验细胞资源共享平台等网站的数据库进行比对,匹配度80%以上即可认为该细胞系正确。
本产品会根据细胞是否正在培养、目的地距离等因素确定运输方式:冷冻的细胞冻存管(干冰)、一小瓶贴壁培养的细胞或一小瓶/管悬浮培养的细胞(常温)。为了更好地耐受长途运输和环境温度等变化,对于正常贴壁培养的细胞,也可能会以悬浮的形式培养在细胞培养瓶或离心管中进行运输。
对于干冰运输的冻存细胞,若干冰已经完全融化,请立即将细胞复苏培养,切勿再次低温冻存;若尚留有干冰,请直接复苏培养或立即将含有细胞的冻存管放入液氮中保存待用,切不可将细胞置于高温环境。
收到冻存的细胞后请尽快复苏细胞进行培养,以确认细胞活力、状态并保种。如暂时不进行复苏操作,冻存细胞可在-80℃条件下保存2个月。
每支冻存管约含1×106个细胞,体积为0.5-1ml,预期存活率60-90%,建议复苏至1个6cm培养皿中。如果复苏后存活率较低,可以消化后转移至3.5cm培养皿中,这样细胞生长会更好。
如果本产品是常温运输,并且是培养瓶中充满完全培养液的贴壁细胞,收到细胞后请在显微镜下观察细胞生长状态,如果细胞密度超过85%请尽快进行传代操作;如果悬浮的细胞较多,请将培养瓶置于培养箱中静置过夜以使悬浮的细胞再次贴壁。如果收到的是常温运输的离心管装的悬浮细胞,可以直接取出转移至培养皿或培养瓶中培养。若培养液颜色正常则保留培养液继续培养,并且在首次更换培养液时,保留一半原培养液,并加入一半新鲜培养液,这样可以尽量避免由于培养液或血清差异导致细胞生长的不适应,确保细胞良好的生长状态。
细胞培养请在生物安全柜台中进行操作,并严格遵守无菌操作。
请在培养液中加入适量青霉素-链霉素溶液以防止可能的细菌污染,如碧云天的青霉素-链霉素溶液(100X) (C0222)。
理论上永生化细胞可无限传代,但为了保证细胞的良好状态,建议最早培养的几代细胞就冻存一批,并每培养一段时间后复苏早期冻存的细胞进行培养。
接收、处理、保存、丢弃及使用细胞的时候要遵守相关法律法规,充分考虑可能存在的风险和责任,采取适当的安全和处理措施尽量降低对健康或环境的危害。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。