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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| D4397-1μg | pFOXO3-TA-Luc (报告基因质粒) | 1μg | 888.00元 |
| D4397-100μg | pFOXO3-TA-Luc (报告基因质粒) | 100μg | 1169.00元 |
pFOXO3-TA-Luc (报告基因质粒)是碧云天自行研发的用于检测FOXO3转录活性水平的报告基因质粒。本质粒是以碧云天的pGL6-TA质粒为模板，在其多克隆位点插入了3个FOXO3结合位点，可以高灵敏度地检测FOXO3的激活水平。
本报告基因质粒的基本信息如下：
| Name | pFOXO3-TA-Luc | Backbone | pGL6-TA |
| cis-element | FOXO3 response element | ||
| Description | pFOXO3-TA-Luc is designed to measure transcriptional activity of Forkhead Box O3.Forkhead box O3, also known as FOXO3 or FOXO3a, is a human protein encoded by the FOXO3 gene. FOXO3 belongs to the O subclass of the forkhead family of transcription factors which are characterized by a distinct fork head DNA-binding domain. There are three other FoxO family members in humans, FOXO1, FOXO4 and FOXO6. These transcription factors share the ability to be inhibited and translocated out of the nucleus on phosphorylation by proteins such as Akt/PKB in the PI3K signaling pathway (aside from FOXO6, which may be constitutively nuclear).[6] Other post-translational modifications including acetylation and methylation are seen and can result in increased or altered FOXO3a activity. FOXO3a also functions as a trigger for apoptosis through upregulation of genes necessary for cell death, such as Bim and PUMA, or downregulation of anti-apoptotic proteins such as FLIP. | ||
pGL6-TA质粒是用于在哺乳动物细胞中进行萤火虫萤光素酶(Firefly luciferase)报告基因检测的新一代质粒。该报告基因质粒比Promega公司的pGL3系列有了全面的改进，一方面对于luciferase的编码进行了改进，确保能更好地在哺乳动物细胞中进行表达，同时对整个质粒中所有的可以被预测出的可能的转录因子结合位点全部进行了适当的突变处理，在保持原有功能不变的情况下，使各种转录因子在质粒上的非特异性结合降到最低。
萤光素、萤光素酶、萤火虫萤光素酶和海肾萤光素酶也经常被称作荧光素、荧光素酶、萤火虫荧光素酶和海肾荧光素酶。
pFOXO3-TA-Luc质粒的图谱如下：
pFOXO3-TA-Luc质粒的主要信息如下：
| Base pairs | 5671 |
| FOXO3 response element | 5526-5591 |
| Luciferase reporter gene | 7-1659 |
| SV40 poly(A) signal | 1703-1824 |
| SV40 promoter | 2018-2375 |
| Neomycin | 2406-3200 |
| Synthetic poly(A) signal | 3225-3273 |
| origin of replication (ori) | 3600-4188 |
| Ampicillin resistance gene | 4388-5248 |
| Synthetic poly(A) signal | 5353-5401 |
| Transcriptional pause site | 5415-5506 |
| Minimal TA promoter (pTA) | 5614-5645 |
pFOXO3-TA-Luc中没有的酶切位点包括：
| AatII | AbsI | AccIII | AccB7I | AclI | AcvI | AflII |
| AjiI | AjuI | AloI | Aor13HI | AscI | AseI | AsiSI |
| AspI | Asp700I | AsuNHI | BarI | BbrPI | BfrI | BmgBI |
| BmtI | BsaI | BsaAI | BsaXI | BseAI | BsePI | BsiWI |
| Bso31I | Bsp13I | Bsp68I | BspEI | BspOI | BspTI | BspTNI |
| BssHII | Bst98I | BstAFI | BstBAI | BstSNI | BtrI | BtuMI |
| Cfr9I | CpoI | CspI | CspCI | Ecl136II | Eco31I | Eco32I |
| Eco53kI | Eco72I | Eco105I | EcoICRI | EcoRI | EcoRV | FauNDI |
| FspAI | I-CeuI | I-PpoI | I-SceI | KflI | Kpn2I | MauBI |
| MluI | MroI | MroXI | MspCI | MssI | NdeI | NheI |
| NruI | PacI | PaeR7I | PalAI | PasI | PauI | PdmI |
| Pfl23II | PflFI | PflMI | PfoI | PI-PspI | PI-SceI | PmaCI |
| PmeI | PmlI | Ppu21I | PshBI | Psp124BI | Psp1406I | PspCI |
| PspLI | PspXI | PsrI | PsyI | PteI | RgaI | RruI |
| RsrII | Rsr2I | SacI | SanDI | SbfI | SdaI | SfaAI |
| Sfr274I | SgfI | SgrDI | SgsI | SlaI | SmaI | SmiI |
| SnaBI | SrfI | Sse8387I | SstI | SwaI | TspMI | Tth111I |
| Van91I | Vha464I | VspI | XcmI | XhoI | XmaI | XmaCI |
| XmnI | ZraI |
pFOXO3-TA-Luc中的单酶切位点包括：
| AarI | Acc65I | AflIII | AhdI | ApaLI | ApoI | BamHI |
| BciVI | BcoDI | BglI | BglII | BpmI | BsmAI | BsmBI |
| BsrGI | BstBI | BstEII | BstXI | BstZ17I | Bsu36I | DraIII |
| EcoNI | Esp3I | HindIII | KpnI | MfeI | MreI | NotI |
| PciI | PsiI | PvuI | SacII | SalI | SfiI | SpeI |
| StuI |
pFOXO3-TA-Luc质粒中推荐使用的测序引物序列如下：
2105-F (5290-5312): 5'-ACTAGTACGTCTCTCAAGGATAA-3'
pFOXO3-TA-Luc的全序列信息:D4397 pFOXO3-TA-Luc (报告基因质粒).txt。
包装清单：| 产品编号 | 产品名称 | 包装 |
| D4397-1µg | pFOXO3-TA-Luc (报告基因质粒) | 1µg |
| D4397-100µg | pFOXO3-TA-Luc (报告基因质粒) | 100µg |
| － | 说明书 | 1份 |
-20℃保存。
注意事项：本质粒未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何个人或单位。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| 产品编号 | 产品名称 | 包装 |
| D2105-1μg | pGL6-TA (报告基因质粒) | 1μg |
| D2105-100μg | pGL6-TA (报告基因质粒) | 100μg |
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| RG009S/M | 萤火虫萤光素酶报告基因检测试剂盒(增强型) | 100/1000次 |
| RG010S/M | 萤火虫萤光素酶报告基因检测试剂盒II (增强型) | 100/1000次 |
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| RG027/RG028 | 双萤光素酶报告基因检测试剂盒 | 100/1000次 |
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| RG055S/M | One-Lumi™萤火虫萤光素酶报告基因检测试剂盒 | 100/1000次 |
| RG056S/M | One-Lumi™ II萤火虫萤光素酶报告基因检测试剂盒 | 100/1000次 |
| RG058S/M | Steady-Lumi™萤火虫萤光素酶报告基因检测试剂盒 | 100/1000次 |
| RG059S/M | Steady-Lumi™ II萤火虫萤光素酶报告基因检测试剂盒 | 100/1000次 |
| RG062S/M | Renilla-Lumi™海肾萤光素酶报告基因检测试剂盒 | 100/1000次 |
| RG066S/M | Renilla-Lumi™ Plus海肾萤光素酶报告基因检测试剂盒 | 100/1000次 |
| RG088S/M | Dual-Lumi™双萤光素酶报告基因检测试剂盒 | 100/1000次 |
| RG089S/M | Dual-Lumi™ II双萤光素酶报告基因检测试剂盒 | 100/1000次 |
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| RG127S/M | 萤火虫萤光素酶报告基因细胞裂解液(增强型) | 10/100ml |
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