碧云天生物技术-EMSA探针－NF-κB (1.75μM)(GS056)

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试剂> 信号转导> 转录调控> EMSA相关

EMSA探针－NF-κB (1.75μM)

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产品编号: GS056

产品包装:60μl

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价格: ¥ 195.00

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产品编号	产品名称	产品包装	产品价格
GS056	EMSA探针－NF-κB (1.75μM)	60μl	195.00元

      EMSA探针－NF-κB是用于EMSA(也称gel shift)研究的NF-κB consensus oligonucleotide。这个双链寡核苷酸含有公认的NF-κB结合位点，可以用作EMSA研究时的探针。
      NF-κB consensus oligo的序列如下：
      5'-AGT TGA GGG GAC TTT CCC AGG C-3'
      3'-TCA ACT CCC CTG AAA GGG TCC G-5'
      本EMSA探针可以使用T4 Polynucleotide Kinase在[γ-³²P]ATP存在的情况下进行标记，也可以用于生物素标记。
      一个包装的探针可以进行约30个同位素标记探针的反应，每次标记的探针可以测定约100个样品；或约可以进行20个生物素标记探针反应。如果作为未标记的探针用于同位素标记探针的冷竞争(cold competition)，可以进行30-60个冷竞争反应。
包装清单：

产品编号	产品名称	包装
GS056	EMSA探针－NF-κB(1.75μM)	60μl
―	说明书	1份

保存条件：
-20℃保存，一年有效。
注意事项：
避免加热到40℃以上，温度过高会导致双链DNA探针解聚成单链。而单链无法用于EMSA研究。
对于EMSA的详细操作可以参考我们的EMSA试剂盒的使用说明。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1. 用于EMSA探针的标记，可以参考如下反应体系：

待标记探针(1.75μM)	2μl
T4 Polynucleotide Kinase 10X Buffer	1μl
Nuclease-Free Water	5μl
[γ-³²P]ATP(3,000Ci/mmol at 10mCi/ml)	1μl
T4 Polynucleotide Kinase (5-10u/μl)	1μl
总体积	10μl

2. 如果用于生物素标记，具体方法和步骤可以参考碧云天的EMSA探针生物素标记试剂盒(GS008)。
3. 如果用于冷竞争，没有标记的探针的用量应该是标记探针的用量的50-100倍。

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