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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| P2461-10μg | Recombinant Human Rac1 (Flag-Tag) | 10μg | 369.00元 |
| P2461-100μg | Recombinant Human Rac1 (Flag-Tag) | 100μg | 1299.00元 |
| P2461-1mg | Recombinant Human Rac1 (Flag-Tag) | 1mg | 8698.00元 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Human | 5879 | P63000 | E.coli | 198aa | ~22kDa | C-Flag Tag |
| 蛋白信息(About this protein) | |
| 名称(Name) | Recombinant Human Rac1，重组人Rac1蛋白 |
| 别名(Synonyms) | RAC1, MIG5, Rac-1, TC-25, p21-Rac1, ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1), Rac family small GTPase 1, MRD48 |
| 产品简介 (Background) |
Beyotime's recombinant human Rac1 (rhRac1) was expressed in E.coli and purified, which contains the mature form Rac1 (2-189aa) with Flag tag (DYKDDDDK) at the C-terminus. Rac1 is involved in the regulation a diverse array of cellular events, including the control of GLUT4 translocation to glucose uptake, cell growth, cytoskeletal reorganization, antimicrobial cytotoxicity and the activation of protein kinases [1]. |
| 产品用途 (Applications) |
Recombinant Human Cdc42 G12V is useful in studying Cdc42 interacting proteins, effectors, GAPs (GTPase-activating proteins) and GEFs (Guanine nucleotide-exchange factors). It can also be used as positive control in Western blot. |
| 外观 (Physical Appearance) |
Liquid |
| 活性(Biological activity) | About 0.28nmol/h/μg in GTPase activity assay. |
| 浓度(Concentration) | 1mg/ml |
| 纯度(Purity) | ≥95% by SDS-PAGE |
| 储存液(Storage buffer) | 50mM Tris pH 7.4, 0.5mM MgCl2, 50mM NaCl, 1mM DTT |
图1. 小GTP酶在“激活”和“静息”状态之间循环的原理图。
小GTP酶(Small GTPase)，也称Small G-proteins、Ras superfamily，是调节真核细胞信号转导、细胞增殖、细胞骨架重组和细胞内膜转运等过程的分子开关，小GTP酶通过结合和水解GTP，在“激活”和“静息”状态之间循环：在外界信号的刺激下，鸟苷酸交换因子(Guanine nucleotide-exchange factors, GEFs)辅助小GTP酶将结合的GDP置换为GTP，小GTP酶结合GTP进入激活状态(Active state)；激活状态的小GTP酶与下游效应蛋白(Effector protein)相互作用，从而刺激细胞发生相应的响应；GTP酶激活蛋白(GTPase-activating proteins, GAPs)催化小GTP酶结合的GTP水解为GDP，并释放自由磷酸盐(Free phosphate, Pi)，此时小GTP酶结合GDP进入静息状态(Inactive state)，鸟嘌呤核苷酸解离抑制蛋白(Guanine nucleotide dissociation inhibitors, GDIs)抑制小GTP酶释放GDP，直到GEFs受到刺激信号再次开启新一轮的循环(图1) [2-3]。
小GTP酶在人类中已发现超过150个家族成员，在果蝇(Drosophila)、秀丽隐杆线虫(C. elegans)、酿酒酵母(S. cerevisiae)、粟酒裂殖酵母(S. pombe)、黏菌(Dictyostelium)和植物中也都发现了保守的同源物。小GTP酶根据结构和功能被分为5个家族分支：Ras家族、Rho家族、Ran家族、Rab家族和Arf家族。Ras家族本身又被分为6个亚家族：Ras亚家族、Ral亚家族、Rit亚家族、Rap亚家族、Rheb亚家族和Rad亚家族[4]。
Rho家族(Ras homologous GTPases)在真核生物中高度保守，是调节肌动蛋白重组的关键因子，因此在细胞迁移、伤口愈合、细胞粘附、细胞极性、膜转运和细胞分裂等过程中发挥着重要作用。目前有21个成员被发现，被分为8亚家族(见下表)，其中RhoA、Rac1和Cdc42研究的最为广泛[5]。
| Subfamily | Rho | Rac | Cdc42 | RhoH | RhoBTB | RhoUV | Rnd | RhoF |
| Rho family members | RhoA RhoB RhoC |
Rac1 Rac2 Rac3 RhoG |
Cdc42 RhoQ (TC10) RhoJ (TCL) |
RhoH | RhoBTB1 RhoBTB2 RhoBTB3 |
RhoU (Wrch) RhoV (Chp) |
Rnd1 Rnd2 Rnd3 (RhoE) |
RhoD RhoF (Rif) |
本产品经SDS-PAGE电泳检测蛋白纯度和分子量参考图2。
图2. 碧云天Recombinant Human Rac1 (Flag-Tag) (P2461)的SDS-PAGE电泳检测图。本蛋白经BeyoGel™ Elite PAGE预制胶(Bis-Tris, 12%, 10孔) (P0849)电泳，Marker为BeyoColor™彩色预染蛋白分子量标准(6.5-270kD) (P0071/P0072)，并经BeyoBlue™考马斯亮蓝超快染色液(P0017F)染色。实际检测结果可能会因样品和检测条件等的不同而存在差异，图中数据仅供参考。
本产品经GTPase Activity Assay Kit (Colorimetric) (P2435)检测GTP酶活性的效果请参考图3。
图3. 碧云天Recombinant Human Rac1 (Flag-Tag) (P2461)的GTP酶活性检测效果图。X轴为Rac1蛋白的质量(μg)，Y轴为对应单位时间内释放Pi摩尔量(nmol/h)，建立二元一次方程的斜率即为GTP酶活性(nmol/h/μg)。实际结果因实验条件、检测仪器等的不同而存在差异，图中数据仅供参考。
包装清单：| 产品编号 | 产品名称 | 包装 |
| P2461-10μg | Recombinant Human Rac1 (Flag-Tag) (1mg/ml) | 10μl |
| P2461-100μg | Recombinant Human Rac1 (Flag-Tag) (1mg/ml) | 100μl |
| P2461-1mg | Recombinant Human Rac1 (Flag-Tag) (1mg/ml) | 1ml |
| — | 说明书 | 1份 |
-20ºC保存，一年有效。-80ºC保存，可以保存更长时间。
注意事项：由于蛋白每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后，须分装后-20ºC或更低温度冻存，尽量避免反复冻融。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| 产品编号 | 产品名称 | 包装 |
| P2061 | Rhotekin-RBD Agarose (活性Rho结合琼脂糖凝胶) | 1ml |
| P2431 | Raf1-RBD Agarose (活性Ras结合琼脂糖凝胶) | 1ml |
| P2437 | PAK1-PBD Agarose (活性Cdc42/Rac结合琼脂糖凝胶) | 1ml |
| P2065 | Active Rho Pull-down and Detection Kit | 50次/250次 |
| P2433 | Active Ras Pull-down and Detection Kit | 50次/250次 |
| P2438 | Active Cdc42 Pull-down and Detection Kit | 50次/250次 |
| P2439 | Active Rac1 Pull-down and Detection Kit | 50次/250次 |
| P2435 | GTP酶活性检测试剂盒(显色法) | 500次/2500次 |
| P2063 | Recombinant Human RhoA (Flag-Tag) | 10μg/100μg/1mg |
| P2401 | Recombinant Human NRas (Flag-Tag) | 10μg/100μg/1mg |
| P2403 | Recombinant Human HRas (His-Tag) | 10μg/100μg/1mg |
| P2405 | Recombinant Human KRas4A (His-Tag) | 10μg/100μg/1mg |
| P2407 | Recombinant Human KRas4B (His-Tag) | 10μg/100μg/1mg |
| P2409 | Recombinant Human KRas4B (G12C, His-Tag) | 10μg/100μg/1mg |
| P2411 | Recombinant Human KRas4B (G12D, His-Tag) | 10μg/100μg/1mg |
| P2413 | Recombinant Human KRas4B (G12V, His-Tag) | 10μg/100μg/1mg |
| P2415 | Recombinant Human KRas4B (G13D, His-Tag) | 10μg/100μg/1mg |
| P2417 | Recombinant Human KRas4B (G13S, His-Tag) | 10μg/100μg/1mg |
| P2419 | Recombinant Human KRas4B (Q61H, His-Tag) | 10μg/100μg/1mg |
| P2451 | Recombinant Human Cdc42 (Flag-Tag) | 10μg/100μg/1mg |
| P2453 | Recombinant Human Cdc42 (Q61L, Flag-Tag) | 10μg/100μg/1mg |
| P2455 | Recombinant Human Cdc42 (G12V, Flag-Tag) | 10μg/100μg/1mg |
| P2461 | Recombinant Human Rac1 (Flag-Tag) | 10μg/100μg/1mg |
| P2463 | Recombinant Human Rac1 (Q61L, Flag-Tag) | 10μg/100μg/1mg |
| P2465 | Recombinant Human Rac1 (T17N, Flag-Tag) | 10μg/100μg/1mg |
| P2469 | Recombinant Human Rac2 (Flag-Tag) | 10μg/100μg/1mg |
| AF0273 | Ras Rabbit Polyclonal Antibody | 50μl |
| AF1168 | Ras Rabbit Monoclonal Antibody | 50μl |
| AF7131 | HRas Rabbit Polyclonal Antibody | 50μl |
| AF7347 | KRas Rabbit Polyclonal Antibody | 50μl |
| AG2391 | KRas Rabbit Monoclonal Antibody | 50μl |
| AG2394 | KRas Mouse Monoclonal Antibody | 50μl |
| AF2794 | CDC42 Rabbit Monoclonal Antibody | 50μl |
| AG3059 | Rac1 Mouse Monoclonal Antibody | 50μl |
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