使用说明：
1. Western检测：
   A. 按照1:500-1000用碧云天提供的Western一抗稀释液稀释抗体。
   B. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动1-2小时，确保稀释的抗体至少
      能在摇动的瞬间覆盖蛋白膜。通常与一抗4℃缓慢摇动过夜效果更好一些。
   C. 回收稀释的一抗，4℃保存以备下次继续使用。
   D. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网
      页： http://www.beyotime.com/western.htm
2. 免疫染色：
    可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操
    作可以参考如下网页： http://www.beyotime.com/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。

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4. Wang ZQ, Wang N, van der Maarel S, Murong SX, Wu ZY.
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10.Fu Y, Teng YY, Xu CW, Zhang X.
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12.Shi H, Zhang Q, Wang Y, Yang P, Wang Q, Li H.
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   Poult Sci. 2011 May;90(5):1037-44.
13.Zhang Y, Dong J, Zhu X, Wang W, Yang Q.
   The effect of sphingomyelin synthase 2 (SMS2) deficiency on the expression of drug
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   Biochem Pharmacol. 2011 Aug 1;82(3):287-94.
14.Zhu ZT, Jiang XS, Wang BC, Meng WX, Liu HY, Tian Y.
   Andrographolide inhibits intimal hyperplasia in a rat model of autogenous vein grafts.
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15.Song H, Yin W, Zeng Q, Jia H, Lin L, Liu X, Mu L, Wang R.
   Hemokinins modulate endothelium function and promote angiogenesis through
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16.Wang D, Fang L, Li K, Zhong H, Fan J, Ouyang C, Zhang H, Duan E, Luo R, Zhang Z,
   Liu X, Chen H, Xiao S.
   Foot-and-mouth disease virus 3C protease cleaves NEMO to impair innate immune signaling.
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17.Wang J, Wu Z, Jin Q.
   COPI Is Required for Enterovirus 71 Replication.
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18.Wang G, Zhan Y, Wang H, Li W.
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19.Yu L, Lu M, Wang P, Chen X.
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20.Zhou Y, Li G, Ji Y, Liu C, Zhu J, Lu Y.
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   Sato Y, Sekido Y, Miao Y, Kondo Y.
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22.Guo X, Li X, Xu Y, Sun T, Yang G, Wu Z, Li E.
   Identification of OASL d, a splice variant of human OASL, with antiviral activity.
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27.Chen J, Xia L, Wu X, Xu L, Nie D, Shi J, Xu X, Ni L, Ju S, Wu X, Zhu H, Shi W.
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   Miao Y, Gao W.
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47.Qiu LL, Wang X, Zhang XH, Zhang Z, Gu J, Liu L, Wang Y, Wang X, Wang SL.
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   Toxicol Lett. 2013 May 23;219(2):116-24. doi: 10.1016/j.toxlet.2013.03.011.
   Epub 2013 Mar 22.
48.Wang J, Du J, Wu Z, Jin Q.
   Quinacrine impairs enterovirus 71 RNA replication by preventing binding of
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   PLoS One. 2013;8(1):e52954. doi: 10.1371/journal.pone.0052954. Epub 2013 Jan 3.
49.Zhou SF, Ma J, Qu HT, Liu ZT, He WD, Wang JD, Dou AX, Zhang N, Liu JL, Guo CS, Shi Y,
   Hou M, Peng J.
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   Epub 2013 Mar 14.
50.Yang Y, Wang YF, Yang XF, Wang ZH, Lian YT, Yang Y, Li XW, Gao X, Chen J, Shu YW,
   Cheng LX, Liao YH, Liu K.
   Specific Kv1.3 blockade modulates key cholesterol-metabolism-associated molecules in
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   J Lipid Res. 2013 Jan;54(1):34-43. doi: 10.1194/jlr.M023846. Epub 2012 Oct 24.
51.Zhang W, Chen J, Tao J, Hu C, Chen L, Zhao H, Xu G, Heng BC, Ouyang HW.
   The promotion of osteochondral repair by combined intra-articular injection of
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   Biomaterials. 2013 Aug;34(25):6046-57. doi: 10.1016/j.biomaterials.2013.04.055.
   Epub 2013 May 20.
52.Zhang X, Jiang H, Gong Q, Fan C, Huang Y, Ling J.
   Expression of high mobility group box 1 in inflamed dental pulp and its chemotactic
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   Biochem Biophys Res Commun. 2014 Aug 8;450(4):1547-52. doi: 10.1016/j.bbrc.
   2014.07.027. Epub 2014 Jul 11.
53.Zhan A, Xu X, Chen L, Wang X, Yanfeng X, Dan W, Zhan Y, Shi Q.
   Decreased expression of Gab2 in patients with temporal lobe epilepsy and pilocarpine-
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   Synapse. 2014 Apr;68(4):168-77. doi: 10.1002/syn.21725. Epub 2013 Dec 20.
54.Ma H, Shi J, Wang C, Guo L, Gong Y, Li J, Gong Y, Yun F, Zhao H, Li E.
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   Biochem Biophys Res Commun. 2014 Aug 8;450(4):1560-7. doi: 10.1016/j.bbrc.
   2014.07.024. Epub 2014 Jul 11.
55.Chen Z, Liu G, Xiao Y, Lu P.
   Adrenomedullin22-52 suppresses high-glucose-induced migration, proliferation, and tube
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   Mol Vis. 2014 Mar 3;20:259-69. eCollection 2014.
56.Zhang Q, Xie D, Wang S, You C, Monroig O, Tocher DR, Li Y.
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   Biochim Biophys Acta. 2014 Jul;1841(7):934-43. doi: 10.1016/j.bbalip.2014.03.009.
   Epub 2014 Mar 28.