使用说明：
1. WST-1溶液的配制：把1毫升电子耦合试剂加入到WST-1粉末中，完全溶解即成WST-1溶液。WST-1溶液4℃
   避光可保存一周，而不影响使用效果。短期内不使用的WST-1溶液，分装后可以-20℃避光保存半年(尽量
   避免反复冻融)。冻存的WST-1溶液溶解后可能会观察到一些沉淀物，这是正常现象，37℃水浴孵育2-10
   分钟，通常可以完全溶解。
2. 通常细胞增殖实验每孔加入100微升2000个细胞，细胞毒性实验每孔加入100微升5000个细胞(具体每孔所
   用的细胞的数目，需根据细胞的大小，细胞增殖速度的快慢等因素决定)。按照实验需要，进行培养并给
   予0-10微升特定的药物刺激。
3. 每孔加入10微升WST-1溶液。如果起始的培养体积为200微升，则需加入20微升WST-1溶液，其它情况以此
   类推。可以用加了相应量细胞培养液和WST-1溶液但没有加入细胞的孔作为空白对照。
4. 在细胞培养箱内继续孵育0.5-4小时，对于大多数情况，孵育1-2小时就可以了。时间长短根据细胞的类
   型和细胞的密度等实验情况而定，初次实验时可以在0.5、1、2和4小时后分别用酶标仪检测，然后选取
   吸光度范围比较适宜的一个时间点用于后续实验。图3为HT-1080细胞在不同时间测定的细胞数量曲线。
                     
            图3. HT-1080细胞培养20小时后，加入WST-1溶液后不同时间测得的吸光度。
5. 把96孔板置于摇床上摇动一分钟，以充分混匀待检测体系。
6. 在450nm测定吸光度。如无450nm滤光片，可以使用420-480nm的滤光片。可以使用大于600nm的波长作为
   参考波长进行双波长测定。

使用本产品的文献：
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   Placenta. 2010;31(1):60-6. Epub 2009 Nov 11.
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   Ju S
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   Int J Nanomedicine. 2011;6:203-11.
15.Hu B, Li C, Lu H, Zhu Z, Du S, Ye M, Tan L, Ren D, Han J, Kan S, Wang J, Jin N
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   expressingmulti-epitopes of foot-and-mouth disease virus and a cholera toxin B subunit.
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   (TSA) in C17.2neural stem cells.
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   proliferation of multiplemyeloma cells.
   Cytokine. 2012 Nov;60(2):505-13. doi: 10.1016/j.cyto.2012.06.317. Epub 2012 Jul 31.
20.Cao X, Zhou M, Wang S, Wang C, Hou L, Luo Y, Chen L.
   Inhibition on hepatitis B virus in vitro of lectin from Musca domestica pupa via the
   activation of NF-κB.
   Virus Res. 2012 Dec;170(1-2):53-8. doi: 10.1016/j.virusres.2012.08.012.
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   The Immunosuppressive Activity of Pseudolaric Acid B on T lymphocytes in vitro.
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   Toxicol In Vitro. 2012 Oct;26(7):1087-92. doi: 10.1016/j.tiv.2012.07.006.
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   Food Chem Toxicol. 2013 May;55:192-201. doi: 10.1016/j.fct.2012.12.052.
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   The protective effects of resveratrol on Schwann cells with toxicity induced by ethanol
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   Evaluation of the immunosuppressive activity of artesunate in vitro and in vivo.
   Int Immunopharmacol. 2013 Jun;16(2):306-12. doi: 10.1016/j.intimp.2013.03.011.
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29.Jing C, Li X, Zhang J, Wang J.
   Responses of the Antioxidant System in QGY-7701 Cells to the Cytotoxicity and Apoptosis
   Induced by 1-Octyl-3-methylimidazolium Chloride.
   J Biochem Mol Toxicol.2013 Jun;27(6):330-6.doi:10.1002/jbt.21495.Epub 2013 May 20.
30.Liu XD, Li XF, Feng XL, Zhou B, Cao RB, Chen PY.
   Effect of sonication on B cell development and immunomodulatory functions of Bursa of
   Fabricius.
   Ultrason Sonochem. 2014 Jul;21(4):1343-8. doi: 10.1016/j.ultsonch.2014.02.012. Epub
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   Effect of Sedum sarmentosum BUNGE extract on aristolochic acid-induced renal tubular
   epithelial cell injury.
   J Pharmacol Sci. 2014;124(4):445-56. Epub 2014 Mar 27.
32.Yu Y, Yang Y, Wang J.
   Anti-apoptotic and apoptotic pathway analysis of arsenic trioxide induced apoptosis in
   human gastric cancer SGC-7901 cells.
   Oncol Rep. 2014 Sep;32(3):973-8. doi: 10.3892/or.2014.3276. Epub 2014 Jun 20.
33.Yin Y, Qin T, Yu Q, Yang Q.
   Bursopentin (BP5) from chicken bursa of fabricius attenuates the immune function of
   dendritic cells.
   Amino Acids. 2014 Jul;46(7):1763-74. doi: 10.1007/s00726-014-1735-x. Epub 2014 Apr 8.
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   Erythropoietin attenuates oxidative stress and apoptosis in Schwann cells isolated from
   streptozotocin-induced diabetic rats.
   J Pharm Pharmacol. 2014 Aug;66(8):1150-60. doi: 10.1111/jphp.12244. Epub 2014 Mar 27.
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   BP5 regulated B cell development promoting anti-oxidant defence.
   Amino Acids. 2014 Jan;46(1):209-22. doi: 10.1007/s00726-013-1620-z. Epub 2013 Nov 29.
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   Quan Z.
   Targeting gallbladder cancer: oncolytic virotherapy with myxoma virus is enhanced by
   rapamycin in vitro and further improved by hyaluronan in vivo.
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   Reactive oxygen species contribute to simulated ischemia/reperfusion-induced autophagic
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   Med Sci Monit. 2014 Jun 19;20:1017-23. doi: 10.12659/MSM.890897.
38.Li R, Feng F, Wang Y, Yang X, Yang X, Yang VC.
   Folic acid-conjugated pH/temperature/redox multi-stimuli responsive polymer microspheres
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39.Weng M, Zhang M, Qin Y, Gong W, Tang Z, Quan Z, Wu K.
   Targeting gallbladder carcinoma: bone marrow-derived stem cells as therapeutic delivery
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   Hydroxypropyl-sulfobutyl-β-cyclodextrin improves the oral bioavailability of edaravone
   by modulating drug efflux pump of enterocytes.
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