使用说明：
1. Mito-Tracker Green储存液的配制：
   用无水DMSO(anhydrous dimethylsulfoxide)配制Mito-Tracker Green至终浓度为1mM。配制后可以-20℃
   或更低温度避光保存。
2. Mito-Tracker Green工作液的配制：
   a. 取少量1mM Mito-Tracker Green储存液按照1:5000-1:50000的比例加入到细胞培养液或适当的溶液
     (例如含钙镁离子的HBSS)中，使最终浓度为20-200nM。例如取1μl Mito-Tracker Green加入到50ml
     或5ml细胞培养液或适当的溶液(例如含钙镁离子的HBSS)中。混匀后即为Mito-TrackerGreen工作液。
      HBSS with Ca²⁺ & Mg²⁺ (C0219)可以向碧云天订购。
   b. Mito-Tracker Green工作液使用前需37℃预温育。
      注：工作液中Mito-Tracker Green的浓度可以根据实际情况进行适当调整。为降低背景，在染色效果
      可以接受的范围内，建议尽量使用较低浓度的Mito-Tracker Green。
3. 线粒体的荧光标记：
   a. 去除细胞培养液，加入步骤2配制好的并37℃预温育的Mito-Tracker Green染色工作液，与细胞37℃
      共孵育15-45分钟。
   b. 去除Mito-Tracker Green染色工作液，加入37℃预温育的新鲜细胞培养液。
   c. 随后通常用荧光显微镜或激光共聚焦显微镜进行观察。此时可观察到线粒体呈明亮的强荧光染色。如
      果染色效果欠佳，可以提高Mito-Tracker Green染色工作液中Mito-Tracker Green的浓度或在推荐的
      时间范围内适当延长染色时间。

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