使用说明：
1. 细胞样品的准备：
   a. 对于贴壁细胞：小心收集细胞培养液到一离心管内备用。用胰酶消化细胞，至细胞可以被轻轻用移
      液管或枪头吹打下来时，加入前面收集的细胞培养液，吹打下所有的贴壁细胞，并轻轻吹散细胞。
      再次收集到离心管内。1000g左右离心3-5分钟，沉淀细胞。对于特定的细胞，如果细胞沉淀不充
      分，可以适当延长离心时间或稍稍加大离心力。小心吸除上清，可以残留约50微升左右的培养液，
      以避免吸走细胞。加入约1毫升冰浴预冷的PBS，重悬细胞，并转移到1.5毫升离心管内。再次离
      心沉淀细胞，小心吸除上清，可以残留约50微升左右的PBS，以避免吸走细胞。轻轻弹击离心管
      底以适当分散细胞，避免细胞成团。
   b. 对于悬浮细胞：1000g左右离心3-5分钟，沉淀细胞。对于特定的细胞，如果细胞沉淀不充分，可以
      适当延长离心时间或稍稍加大离心力。小心吸除上清，可以残留约50微升左右的培养液，以避免吸
      走细胞。加入约1毫升冰浴预冷的PBS，重悬细胞，并转移到1.5毫升离心管内。再次离心沉淀细胞，
      小心吸除上清，可以残留约50微升左右的PBS，以避免吸走细胞。轻轻弹击离心管底以适当分散细
      胞，避免细胞成团。
2. 细胞固定：加入1毫升冰浴预冷70%乙醇中，轻轻吹打混匀，4℃固定2小时或更长时间。固定12-24小时
   可能效果更佳。1000g左右离心3-5分钟，沉淀细胞。对于特定的细胞，如果细胞沉淀不充分，可以适
   当延长离心时间或稍稍加大离心力。小心吸除上清，可以残留约50微升左右的70%乙醇，以避免吸走
   细胞。加入约1毫升冰浴预冷的PBS，重悬细胞。再次离心沉淀细胞，小心吸除上清，可以残留约50微
   升左右的PBS，以避免吸走细胞。轻轻弹击离心管底以适当分散细胞，避免细胞成团。
3. 碘化丙啶染色液的配制：参考下表，根据待检测样品的数量配制适量的碘化丙啶染色液：

   注：配制好的碘化丙啶染色液短时间内可以4℃保存，宜当日使用。
4. 染色：每管细胞样品中加入0.5毫升碘化丙啶染色液，缓慢并充分重悬细胞沉淀，37℃避光温浴30分
   钟。随后可以4℃或冰浴避光存放。染色完成后宜在24小时内完成流式检测，最好能在当日完成流式检
   测。
5. 流式检测和分析：用流式细胞仪在激发波长488nm波长处检测红色荧光，同时检测光散射情况。采用适
   当分析软件进行细胞DNA含量分析和光散射分析。

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   Annexin A2 silencing inhibits invasion, migration, and tumorigenic potential of
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   Emodin inhibits breast cancer cell proliferation through the ERα-MAPK/Akt-cyclin
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   shRNA-Mediated EMMPRIN Silencing Inhibits Human Leukemic Monocyte Lymphoma U937 Cell
   Proliferation and Increases Chemosensitivity to Adriamycin.
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   A recombinant trans-membrane protein hMnSOD-R9 inhibits the proliferation of cervical
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   Biodegradable self-assembled nanoparticles of poly (D,L-lactide-co-glycolide)/hyaluronic
   acid block copolymers for target delivery of docetaxel to breast cancer.
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   Reprogramming sertoli cells into pluripotent stem cells.
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   Expression and distribution of immunoglobulin G in the normal liver, hepatocarcinoma and
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   Z, Gao F, He Q.
   Increased delivery of doxorubicin into tumor cells using extracellularly activated TAT
   functionalized liposomes: in vitro and in vivo study.
   J Biomed Nanotechnol. 2014 Aug;10(8):1563-73.