使用说明：
  对于切片或细胞样品，在固定后用免疫染色洗涤液洗涤一次(注：石蜡切片需先脱蜡)。然后就可以用免疫染色封闭液进行封闭。通常建议封闭60分钟。封闭时可以在摇床上缓慢摇动。如果样品在摇动时容易脱落，也可以直接放置在桌面上不进行任何摇动，效果会略差一些，但也可以满足常规的要求。完成封闭后就可以进行一抗孵育等后续操作。
    详细的免疫染色操作可以参考我们的相关网页：http://www.beyotime.com/immunol-staining.htm

使用本产品的文献：
1. Zou XH, Li HM, Wang S, Leski M, Yao YC, Yang XD, Huang QJ, Chen GQ.
   The effect of 3-hydroxybutyrate methyl ester on learning and memory in mice.
   Biomaterials. 2009 Mar;30(8):1532-41.
2. Zhang Z, Wang Q, Li P, Zhou Y, Li S, Yi W, Chen A, Kong P, Hu C.
   Overexpression of the Interferon regulatory factor 4-binding protein in human colorectal
   cancer and its clinical significance.
   Cancer Epidemiol. 2009 Aug;33(2):130-6. Epub 2009 Jul 10.
3. Tang S, Wang Y, Zhang D, Gao Y, Ma Y, Yin B, Sun J, Liu J, Zhang Y.
   Reprogramming donor cells with oocyte extracts improves in vitro development of nuclear
   transfer embryos.
   Anim Reprod Sci. 2009 Oct;115(1-4):1-9.
4. Zhou F, Hu J, Shao JH, Zou SB, Shen SL, Luo ZQ
   Metronomic chemotherapy in combination with antiangiogenic treatment induces mosaic
   vascularreduction and tumor growth inhibition in hepatocellular carcinoma xenografts.
   J Cancer Res Clin Oncol. 2012 Jun 27.
5. Su J, Wang Y, Li R, Peng H, Hua S, Li Q, Quan F, Guo Z, Zhang Y.
   Oocytes selected using BCB staining enhance nuclear reprogramming and the in vivo
   development of SCNTembryos in cattle.
   PLoS One. 2012;7(4):e36181. doi: 10.1371/journal.pone.0036181. Epub 2012 Apr 27.
6. Yang Z, Liu J, Liu H, Qiu M, Liu Q, Zheng L, Pang M, Quan F, Zhang Y.
   Isolation and Characterization of SSEA3(+) Stem Cells Derived from Goat Skin
   Fibroblasts.
   Cell Reprogram. 2013 Jun;15(3):195-205. doi: 10.1089/cell.2012.0080.
   Epub 2013 May 13.
7. Peng H, Zhang W, Xiao T, Zhang Y.
   Nlrp4g is an oocyte-specific gene but is not required for oocyte maturation in the
   mouse.
   Reprod Fertil Dev. 2013 Jun 11. doi: 10.1071/RD12409.
8. Liu J, Wang Y, Su J, Wang L, Li R, Li Q, Wu Y, Hua S, Quan F, Guo Z, Zhang Y.
   Effect of the time interval between fusion and activation on epigenetic reprogramming
   and development of bovinesomatic cell nuclear transfer embryos.
   Cell Reprogram. 2013 Apr;15(2):134-42. doi: 10.1089/cell.2012.0052. Epub 2013 Mar 5.
9. Qiu M, Quan F, Han C, Wu B, Liu J, Yang Z, Su F, Zhang Y.
   Effects of granulosa cells on steroidogenesis, proliferation and apoptosis of stromal
   cells and theca cells derivedfrom the goat ovary.
   J Steroid Biochem Mol Biol. 2013 Jun 28. pii: S0960-0760(13)00120-9. doi: 10.1016/
   j.jsbmb.2013.06.005.
10. Cong ZX, Wang HD, Wang JW, Zhou Y, Pan H, Zhang DD, Zhu L.
   ERK and PI3K signaling cascades induce Nrf2 activation and regulate cell viability
   partly through Nrf2 in humanglioblastoma cells.
   Oncol Rep. 2013 Aug;30(2):715-22. doi: 10.3892/or.2013.2485. Epub 2013 May 23.
11.Wang J, Lu K, Liang F, Li X, Wang L, Yang C, Yan Z, Zhang S, Liu H.
   Decreased autophagy contributes to myocardial dysfunction in rats subjected to nonlethal
   mechanical trauma.
   PLoS One. 2013 Aug 19;8(8):e71400. doi: 10.1371/journal.pone.0071400. eCollection
   2013.
12.Liu SL, Zhong SS, Ye DX, Chen WT, Zhang ZY, Deng J.
   Repression of G protein-coupled receptor family C group 5 member A is associated with
   pathologic differentiation grade of oral squamous cell carcinoma.
   J Oral Pathol Med. 2013 Nov;42(10):761-8. doi: 10.1111/jop.12077. Epub 2013 May 7.
13.Zhang L, Wang H, Zhu J, Xu J, Ding K.
   Mollugin induces tumor cell apoptosis and autophagy via the PI3K/AKT/mTOR/p70S6K and ERK
   signaling pathways.
   Biochem Biophys Res Commun. 2014 Jul 18;450(1):247-54. doi: 10.1016/j.bbrc.
   2014.05.101. Epub 2014 Jun 2.
14.Cong ZX, Wang HD, Zhou Y, Wang JW, Pan H, Zhang DD, Zhang L, Zhu L.
   Temozolomide and irradiation combined treatment-induced Nrf2 activation increases
   chemoradiation sensitivity in human glioblastoma cells.
   J Neurooncol. 2014 Jan;116(1):41-8.
15.Qiu M, Liu J, Han C, Wu B, Yang Z, Su F, Quan F, Zhang Y.
   The influence of ovarian stromal/theca cells during in vitro culture on steroidogenesis,
   proliferation and apoptosis of granulosa cells derived from the goat ovary.
   Reprod Domest Anim. 2014 Feb;49(1):170-6. doi: 10.1111/rda.12256. Epub 2013 Nov 21.
16.Qin Q, Cheng H, Lu J, Zhan L, Zheng J, Cai J, Yang X, Xu L, Zhu H, Zhang C, Liu J, Ma J,
   Zhang X, Dai S, Sun X.
   Small-molecule survivin inhibitor YM155 enhances radiosensitization in esophageal
   squamous cell carcinoma by the abrogation of G2 checkpoint and suppression of homologous
   recombination repair.
   J Hematol Oncol. 2014 Aug 20;7:62. doi: 10.1186/s13045-014-0062-8.
17.Zhu Y, Zhang JJ, Xie KL, Tang J, Liang WB, Zhu R, Zhu Y, Wang B, Tao JQ, Zhi XF, Li Z,
   Gao WT, Jiang KR, Miao Y, Xu ZK.
   Specific-detection of clinical samples, systematic functional investigations, and
   transcriptome analysis reveals that splice variant MUC4/Y contributes to the malignant
   progression of pancreatic cancer by triggering malignancy-related positive feedback
   loops signaling.
   J Transl Med. 2014 Nov 4;12(1):309.
18.Zhang L, Wang H, Xu J, Zhu J, Ding K.
   Inhibition of cathepsin S induces autophagy and apoptosis in human glioblastoma cell
   lines through ROS-mediated PI3K/AKT/mTOR/p70S6K and JNK signaling pathways.
   Toxicol Lett. 2014 Aug 4;228(3):248-59. doi: 10.1016/j.toxlet.2014.05.015. Epub 2014
   May 27.
19.Hui K, Yang Y, Shi K, Luo H, Duan J, An J, Wu P, Ci Y, Shi L, Xu C.
   The p38 MAPK-regulated PKD1/CREB/Bcl-2 pathway contributes to selenite-induced
   colorectal cancer cell apoptosis in vitro and in vivo.
   Cancer Lett. 2014 Nov 1;354(1):189-99. doi: 10.1016/j.canlet.2014.08.009. Epub 2014
   Aug 12.
20.Song ZQ, Yang LF, Wang YS, Zhu T, Zhou XM, Yin XM, Yao HQ, Zhao DM.
   Overexpression of BAT3 alleviates prion protein fragment PrP106-126-induced neuronal
   apoptosis.
   CNS Neurosci Ther. 2014 Aug;20(8):737-47. doi: 10.1111/cns.12243. Epub 2014 Mar 15.
21.Gao W, Gu Y, Li Z, Cai H, Peng Q, Tu M, Kondo Y, Shinjo K, Zhu Y, Zhang J, Sekido Y, Han
   B, Qian Z, Miao Y.
   miR-615-5p is epigenetically inactivated and functions as a tumor suppressor in
   pancreatic ductal adenocarcinoma.
   Oncogene. 2014 Apr 28;0. doi: 10.1038/onc.2014.101.