使用说明：
  对于细胞样品，去除培养液后，按照每六孔板一个孔加入1毫升固定液的比例，加入免疫染色固定液。对于其它样品，加入免疫染色固定液的量都以充分盖住样品为准。
    通常室温固定10分钟即可完成固定。但固定较长时间，例如4℃固定过夜也没有任何问题。
    详细的免疫染色操作可以参考我们的相关网页：http://www.beyotime.com/immunol-staining.htm。

使用本产品的文献：
1. Tang S, Wang Y, Zhang D, Gao Y, Ma Y, Yin B, Sun J, Liu J, Zhang Y.
   Reprogramming donor cells with oocyte extracts improves in vitro development of nuclear
   transfer embryos.
   Anim Reprod Sci. 2009 Oct;115(1-4):1-9.
2. Lin J, Lin X, Yang GH, Wang Y, Peng BW, Lin JY.
   Toxoplasma gondii: expression of GRA1 gene in endoplasmic reticulum promotes both growth
   and adherence and modulates intracellular calcium release in macrophages.
   Exp Parasitol. 2010;125(2):165-71. Epub 2010 Feb 1.
3. Wang X, Zhao Y, Fu Z, He Y, Xiang D, Zhang L.
   Prelining autogenic endothelial cells in allogeneic vessels inhibits thrombosis and
   intimalhyperplasia: an efficacy study in dogs.
   J Surg Res. 2011 Jul;169(1):148-55.
4. Wang PH, Gu ZH, Wan DH, Zhang MY, Weng SP, Yu XQ, He JG.
   The shrimp NF-κB pathway is activated by white spot syndrome virus (WSSV) 449 to
   facilitate theexpression of WSSV069 (ie1), WSSV303 and WSSV371.
   PLoS One. 2011;6(9):e24773. Epub 2011 Sep 12.
5. Wang PH, Wan DH, Gu ZH, Deng XX, Weng SP, Yu XQ, He JG
   Litopenaeus vannamei tumor necrosis factor receptor-associated factor 6 (TRAF6) responds
   toVibrio alginolyticus and white spot syndrome virus (WSSV) infection and activates
   antimicrobial peptide genes.
   Dev Comp Immunol. 2011 Jan;35(1):105-14.
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   Molecular cloning, characterization and expression analysis of two novel Tolls
   (LvToll2 andLvToll3) and three putative Spätzle-like Toll ligands (LvSpz1-3) from
   Litopenaeus vannamei.
   Dev Comp Immunol. 2012 Feb;36(2):359-71.
7. Wang PH, Wan DH, Pang LR, Gu ZH, Qiu W, Weng SP, Yu XQ, He JG.
   Molecular cloning, characterization and expression analysis of the tumor necrosis factor
   (TNF)superfamily gene, TNF receptor superfamily gene and
   lipopolysaccharide-induced TNF-α factor(LITAF) gene from Litopenaeus vannamei.
   Dev Comp Immunol. 2012 Jan;36(1):39-50.
8. Su J, Wang Y, Li R, Peng H, Hua S, Li Q, Quan F, Guo Z, Zhang Y.
   Oocytes selected using BCB staining enhance nuclear reprogramming and the in vivo
   development of SCNTembryos in cattle.
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   Molecular cloning, characterization and expression analysis of two novel Tolls (LvToll2
   and LvToll3) and threeputative Sp?tzle-like Toll ligands (LvSpz1-3) from Litopenaeus
   vannamei.
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   Molecular cloning, characterization and expression analysis of the tumor necrosis factor
   (TNF) superfamily gene, TNF receptor superfamily gene and lipopolysaccharide-induced
   TNF-α factor (LITAF) gene from Litopenaeus vannamei.
   Dev Comp Immunol. 2012 Jan;36(1):39-50. doi: 10.1016/j.dci.2011.06.002.
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   Mechanical stretch modulates microRNA 21 expression, participating in proliferation and
   apoptosis in culturedhuman aortic smooth muscle cells.
   PLoS One. 2012;7(10):e47657. doi: 10.1371/journal.pone.0047657. Epub 2012 Oct 17.
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   Identification, characterization, and function analysis of the Cactus gene from
   Litopenaeus vannamei.
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   Isolation and Characterization of SSEA3(+) Stem Cells Derived from Goat Skin
   Fibroblasts.
   Cell Reprogram. 2013 Jun;15(3):195-205. doi: 10.1089/cell.2012.0080.
   Epub 2013 May 13.
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   Improving development of cloned goat embryos by supplementing α-lipoic acid to oocyte
   in vitro maturationmedium.
   Theriogenology. 2013 Aug;80(3):228-33. doi: 10.1016/j.theriogenology.2013.03.027.
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   Theriogenology. 2013 Jun 7. pii: S0093-691X(13)00166-0.
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   Nlrp4g is an oocyte-specific gene but is not required for oocyte maturation in the
   mouse.
   Reprod Fertil Dev. 2013 Jun 11. doi: 10.1071/RD12409.
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   Identification and function of leucine-rich repeat flightless-I-interacting protein 2
   (LRRFIP2) in Litopenaeusvannamei.
   PLoS One. 2013;8(2):e57456. doi: 10.1371/journal.pone.0057456. Epub 2013 Feb 28.
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   Litopenaeus vannamei Toll-interacting protein (LvTollip) is a potential negative
   regulator of the shrimp Toll pathway involved in the regulation of the shrimp
   antimicrobial peptide gene penaeidin-4 (PEN4).
   Dev Comp Immunol. 2013 Jul-Aug;40(3-4):266-77. doi: 10.1016/j.dci.2013.02.011.
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19.Luo XC, Wang SS, Zhang J, Qian DD, Wang SM, Li LL.
   Effects of Early or Overexpression of the Autographa californica Multiple
   Nucleopolyhedrovirus orf94 (ODV-e25) on Virus Replication.
   PLoS One. 2013 Jun 18;8(6):e65635. Print 2013.
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   Litopenaeus vannamei sterile-alpha and armadillo motif containing protein (LvSARM) is
   involved in regulation of Penaeidins and antilipopolysaccharide factors.
   PLoS One. 2013;8(2):e52088. doi: 10.1371/journal.pone.0052088. Epub 2013 Feb 6.
21.Zhou C, Li C, Li D, Wang Y, Shao W, You Y, Peng J, Zhang X, Lu L, Shen X.
   BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein regulates neurite
   development via PI3K-AKT and ERK signaling pathways.
   Neuroscience. 2013 Dec 19;254:361-8. doi: 10.1016/j.neuroscience.2013.09.045. Epub
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22.Wang PH, Wan DH, Chen YG, Weng SP, Yu XQ, He JG.
   Characterization of four novel caspases from Litopenaeus vannamei (Lvcaspase2-5) and
   their role in WSSV infection through dsRNA-mediated gene silencing.
   PLoS One. 2013 Dec 23;8(12):e80418. doi: 10.1371/journal.pone.0080418. eCollection
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23.Wang PH, Gu ZH, Wan DH, Liu BD, Huang XD, Weng SP, Yu XQ, He JG.
   The shrimp IKK-NF-κB signaling pathway regulates antimicrobial peptide expression and
   may be subverted by white spot syndrome virus to facilitate viral gene expression.
   Cell Mol Immunol. 2013 Sep;10(5):423-36. doi: 10.1038/cmi.2013.30. Epub 2013 Aug 19.
24.Zhang Y, Jiang Y, Lian X, Xu S, Wei J, Chu C, Wang S.
   Effects of ERα-specific antagonist on mouse preimplantation embryo development and
   zygotic genome activation.
   J Steroid Biochem Mol Biol. 2014 Sep 26;145C:13-20. doi: 10.1016/j.jsbmb.
   2014.09.023.
25.Chen Z, Zhu M, Pan X, Zhu Y, Yan H, Jiang T, Shen Y, Dong X, Zheng N, Lu J, Ying S, Shen
   Y.
   Inhibition of Hepatitis B virus replication by SAMHD1.
   Biochem Biophys Res Commun. 2014 Aug 8;450(4):1462-8. doi: 10.1016/j.bbrc.
   2014.07.023. Epub 2014 Jul 11.
26.Chen S, An FM, Yin L, Liu AR, Yin DK, Yao WB, Gao XD.
   Glucagon-like peptide-1 protects hippocampal neurons against advanced glycation end
   product-induced tau hyperphosphorylation.
   Neuroscience. 2014 Jan 3;256:137-46. doi: 10.1016/j.neuroscience.2013.10.038. Epub
   2013 Oct 30.
27.Li P, Ji M, Lu F, Zhang J, Li H, Cui T, Li Wang X, Tang D, Ji C.
   Degradation of AF1Q by chaperone-mediated autophagy.
   Exp Cell Res. 2014 Sep 10;327(1):48-56. doi: 10.1016/j.yexcr.2014.05.013. Epub 2014
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28.Hui K, Yang Y, Shi K, Luo H, Duan J, An J, Wu P, Ci Y, Shi L, Xu C.
   The p38 MAPK-regulated PKD1/CREB/Bcl-2 pathway contributes to selenite-induced
   colorectal cancer cell apoptosis in vitro and in vivo.
   Cancer Lett. 2014 Nov 1;354(1):189-99. doi: 10.1016/j.canlet.2014.08.009. Epub 2014
   Aug 12.
29.Xu H, Zhang W, Li Y, Ye FF, Yin PP, Yu X, Hu MN, Fu YS, Wang C, Shang de J.
   The Bifunctional Liposomes Constructed by Poly(2-ethyl-oxazoline)-cholesteryl Methyl
   Carbonate: an Effectual Approach to Enhance Liposomal Circulation Time, pH-Sensitivity
   and Endosomal Escape.
   Pharm Res. 2014 Nov;31(11):3038-50. doi: 10.1007/s11095-014-1397-0. Epub 2014 May 8.
30.Huang B, Jin M, Yan H, Cheng Y, Huang D, Ying S, Zhang L.
   Simvastatin enhances oxidized-low density lipoprotein-induced macrophage autophagy and
   attenuates lipid aggregation.
   Mol Med Rep. 2014 Oct 27. doi: 10.3892/mmr.2014.2790.
31.Zhu Y, Zhang JJ, Xie KL, Tang J, Liang WB, Zhu R, Zhu Y, Wang B, Tao JQ, Zhi XF, Li Z,
   Gao WT, Jiang KR, Miao Y, Xu ZK.
   Specific-detection of clinical samples, systematic functional investigations, and
   transcriptome analysis reveals that splice variant MUC4/Y contributes to the malignant
   progression of pancreatic cancer by triggering malignancy-related positive feedback
   loops signaling.
   J Transl Med. 2014 Nov 4;12(1):309.
32.Fu M, Wu X, He J, Zhang Y, Hua S.
   Natrium fluoride influences methylation modifications and induces apoptosis in mouse
   early embryos.
   Environ Sci Technol. 2014 Sep 2;48(17):10398-405. doi: 10.1021/es503026e. Epub 2014
   Aug 14.
33.Li YW, Mo XB, Zhou L, Li X, Dan XM, Luo XC, Li AX.
   Identification of IRAK-4 in grouper (Epinephelus coioides) that impairs MyD88-dependent
   NF-κB activation.
   Dev Comp Immunol. 2014 Jul;45(1):190-7. doi: 10.1016/j.dci.2014.02.019. Epub 2014 Mar
   11.