MTT细胞增殖及细胞毒性检测试剂盒

 产品编号: C0009
 产品包装: 500次
 产品价格: 246.00元
说明书下载
加入购物车

产品简介

产品简介:

产品编号 产品名称 产品包装 产品价格
C0009 MTT细胞增殖及细胞毒性检测试剂盒 500次 246.00元

    MTT细胞增殖及细胞毒性检测试剂盒(MTT Cell Proliferation and Cytotoxicity Assay Kit)被广泛应用于细胞增殖和细胞毒性的检测。MTT可以被线粒体内的一些脱氢酶还原生成结晶状的深紫色产物formazan。在特定溶剂存在的情况下,可以被完全溶解。然后通过酶标仪可以测定570nm波长附近的吸光度。细胞增殖越多越快,则吸光度越高;细胞毒性越大,则吸光度越低。
    本试剂盒采用了独特的Formazan溶解液配方,无需去除原有的培养液,可以直接加入Formazan溶解液溶解formazan。从而避免了由于去除培养液时formazan被部分去除而引起的误差。
    本试剂盒本底低,灵敏度高,线性范围宽,使用方便。
    关于不同细胞增殖和细胞毒性检测试剂盒的比较和选择请参考:
    http://www.beyotime.com/mtt-comp.htm
    本试剂盒可以测定500个样品。
包装清单:

产品编号

产品名称

包装

C0009-1

MTT

25mg

C0009-2

MTT溶剂

5ml

C0009-3

Formazan溶解液

55ml

说明书

1份

保存条件:
    4℃或-20℃保存,MTT需避光保存,一年有效;MTT配制成溶液后需-20℃避光保存;Formazan溶解液也可以室温保存。
注意事项:
    由于使用96孔板进行检测,如果细胞培养时间较长,一定要注意蒸发的问题。一方面,由于96孔板周围一圈最容易蒸发,可以采取弃用周围一圈的办法,改加PBS,水或培养液;另一方面,可以把96孔板置于靠近培养箱内水源的地方,以缓解蒸发。
    MTT溶剂在低温情况下会凝固,使用前请室温放置或20-25℃水浴温育片刻至全部融解后使用。
    MTT配制成溶液后为黄色,需避光保存,长时间光照会导致失效。当颜色变为灰绿色时,请勿使用。MTT溶液在4℃、冰浴等较低温度情况下会凝固,可以20-25℃水浴温育片刻至全部融解后使用。
    Formazan溶解液结冻或产生沉淀时可以37℃水浴孵育以促进溶解,并且必须在全部溶解并混匀后使用。
    为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明

使用说明:
1. MTT溶液的配制:用5ml MTT溶剂溶解25mg MTT,配制成5mg/ml的MTT溶液。配制后即可使用,或直接
   -20℃避光保存,也可以根据需要适当分装后-20℃避光保存。
2. 通常细胞增殖实验每孔加入100微升2000个细胞,细胞毒性实验每孔加入100微升5000个细胞(具体每孔所
   用的细胞的数目,需根据细胞的大小,细胞增殖速度的快慢等决定)。按照实验需要,进行培养并给予
   0-10微升特定的药物刺激。
3. 每孔加入10微升MTT溶液,在细胞培养箱内继续孵育4小时。
4. 每孔加入100微升Formanzan溶解液,在细胞培养箱内再继续孵育。直至在普通光学显微镜下观察发现
   formazan全部溶解。通常37℃孵育4小时左右,紫色结晶会全部溶解。如果紫色结晶较小较少,溶解的时
   间会短一些。如果紫色结晶较大较多,溶解的时间会长一些。
5. 在570nm测定吸光度。如无570nm滤光片,可以使用560-600nm的滤光片。

产品图片

 


相关产品


    

 

 

 

  相关产品请点击如下按钮:

 

细胞凋亡坏死

 

细胞增殖


相关论文

使用本产品的相关论文:
1. Zhong Y, Zhang XH, Chen J, Chi Z, Sun B, Li Y, Austin B.
   Overexpression, purification, characterization, and pathogenicity of Vibrio harveyi
   hemolysin VHH.

   Infect Immun. 2006 Oct;74(10):6001-5.
2. Li QT, Zhu YZ, Chu JY, Dong K, He P, Feng CY, Hu BY, Zhang SM, Guo XK.
   Granulocyte-macrophage colony-stimulating factor DNA prime-protein boost strategy to
   enhance efficacy of a recombinant pertussis DNA vaccine
.
   Acta Pharmacol Sin. 2006 Nov;27(11):1487-94.
3. Yang H, Chen J, Yang G, Zhang XH, Li Y.
   Mutational analysis of the zinc metalloprotease EmpA of Vibrio anguillarum.
   FEMS Microbiol Lett. 2007 Feb;267(1):56-63.
4. Yang H, Chen J, Yang G, Zhang XH, Li Y, Wang M.
   Characterization and pathogenicity of the zinc metalloprotease empA of Vibrio
   anguillarum expressed in Escherichia coli.

   Curr Microbiol. 2007 Mar;54(3):244-8.
5. Li ZJ, Zhang K, Lou SQ, Zheng YF.
   A Cellular Study on Cytotoxicity of Magnesium- calcium Alloy.
   Chinese journal of bone and joint injury. Sep,2007;Vol.22 NO.9.
6. Xu J, Sun Y, Huang J, Chen C, Liu G, Jiang Y, Zhao Y, Jiang Z.
   Photokilling cancer cells using highly cell-specific antibody-TiO(2) bioconjugates and
   electroporation.

   Bioelectrochemistry. 2007 Nov;71(2):217-22.
7. Fan QY, Liu QH, Li CP, Wang J.
   Experimental study on the effect of protoporphyrin disodium in vitro on anti-hbv.
   Modern preventive medicine.2007, Vol.34 No.19.
8. Bai L, Pang WJ, Yang YJ, Yang GS.
   Modulation of Sirt1 by resveratrol and nicotinamide alters proliferation and
   differentiation of pig preadipocytes.

   Mol Cell Biochem. 2008 Jan;307(1-2):129-40.
9. Zhou XM, Xu GX, Zhao DM.
   In vitro effect of prion peptide PrP 106-126 on mouse macrophages: Possible role of
   macrophages in transport and proliferation for prion protein.

   Microb Pathog. 2008 Feb;44(2):129-34.
10.Zhan XD, Wang KX, Li CP.
   Studies on Anti2HBV Effect in vitro of Snail Polysaccharide.
   Chinese Journal of Experimental Traditional Medical Formulae.Mar.2008;Vol.14,
   No.3.
11.Tian Y, Wang Q, Liu Q, Ma Y, Cao X, Zhang Y.
   Role of RpoS in stress survival, synthesis of extracellular autoinducer 2, and virulence
   in Vibrio alginolyticus.

   Arch Microbiol. 2008 Jul 19.
12.LI Y, GUAN L, YANG J, ZHOU X, YIN X, ZHAO D.
   Influence of PrP 106―126 on expression of laminin and fibronectin in astrocyte.
   Chinese Science Bulletin. July 2008,vol. 53 no. 14,2160-2164.
13.Zhang Y, Jiang W, Fan Y, Wen J, Hao W, Qian M.
   Engineering enhancement of the immune response to HBV DNA vaccine in mice by the use of
   LIGHT gene adjuvant.

   J Virol Methods. 2008 Nov;153(2):142-8. Epub 2008 Sep 3.
14.Zhang WW, Hu YH, Wang HL, Sun L.
   Identification and characterization of a virulence-associated protease from a pathogenic
   Pseudomonas fluorescens strain.

   Vet Microbiol. 2009 Oct 20;139(1-2):183-8.
15.Fan Y, Jiang WZ, Wen JJ, Hao WL, Du JN, Liu X, Qian M.
   B7-DC-silenced dendritic cells induce stronger anti-HBV immunity in transgenic mice.
   Arch Virol. 2009;154(11):1813-21. Epub 2009 Oct 17.
16.Sun K, Jiao XD, Zhang M, Sun L.
   DNA adenine methylase is involved in the pathogenesis of Edwardsiella tarda.
   Vet Microbiol. 2010 Feb 24;141(1-2):149-54.
17.Wang YN, Wu W, Chen HC, Fang H.
   Genistein protects against UVB-induced senescence-like characteristics in human dermal
   fibroblast by p66Shc down-regulation.

   J Dermatol Sci. 2010;58(1):19-27. Epub 2010 Feb 16.
18.Xu CQ, Liu BJ, Wu JF, Xu YC, Duan XH, Cao YX, Dong JC.
   Icariin attenuates LPS-induced acute inflammatory responses: involvement of PI3K/Akt and
   NF-kappaB signaling pathway.

   Eur J Pharmacol. 2010;642(1-3):146-53. Epub 2010 May 25.
19.Chen R, Huang C, Ke Q, He C, Wang H, Mo X.
   Preparation and characterization of coaxial electrospun thermoplastic
   polyurethane/collagen compound nanofibers for tissue engineering applications.

   Colloids Surf B Biointerfaces. 2010;79(2):315-25. Epub 2010 Apr 3.
20.Zheng WJ, Hua YH, Zhang M, Sun L.
   Analysis of the expression and antioxidative property of a peroxiredoxin 6 from
   Scophthalmus maximus.

   Fish &Shellfish Immunology.29(2010)305e311.
21.Hu YH, Zheng WJ, Sun L.
   Identification and molecular analysis of a ferritin subunit from red drum (Sciaenops
   ocellatus).

   Fish Shellfish Immunol. 2010;28(4):678-86. Epub 2010 Jan 10.
22.Cheng S, Zhang WW, Zhang M, Sun L.
   Evaluation of the vaccine potential of a cytotoxic protease and a protective immunogen
   from a pathogenic Vibrio harveyi strain.

   Vaccine. 2010;28(4):1041-7. Epub 2009 Nov 6.
23.Su Y, Li X, Liu Y, Su Q, Qiang ML, Mo X
   Encapsulation and Controlled Release of Heparin from Electrospun
   Poly(L-Lactide-co-epsilon-Caprolactone) Nanofibers.

   J Biomater Sci Polym Ed. 2010 Jun 16.
24.Li X, Lin L, Kanjwal MA, Chronakis IS, Liu S, Chen Y.
   Preparing photochromic nanofibers and animal cells using a photochromic compound of
   1',3',3'-trimethyl-6-nitrospiro (2H-1-benzopyran-2,2'-indoline).

   Colloids Surf B Biointerfaces. 2012 Jan 1;89:67-72.
25.Qian YF, Zhang KH, Chen F, Ke QF, Mo XM
   Cross-linking of gelatin and chitosan complex nanofibers for tissue-engineering
   scaffolds.

   J Biomater Sci Polym Ed. 2011;22(8):1099-113.
26.Ma X, Zhang S, Zhou J, Chen B, Shang Y, Gao T, Wang X, Xie H, Chen F
   Clone-derived human AF-amniotic fluid stem cells are capable of skeletal myogenic
   differentiationin vitro and in vivo.

   J Tissue Eng Regen Med.2012 Aug;6(8):598-613.doi:10.1002/term.462.Epub 2012 Mar 7.
27.Wei D, Sun N, Nan G, Wang Y, Liu HQ, Peeters B, Chen ZN, Bian H.
   Construction of recombinant Newcastle disease virus Italien strain for oncolytic
   virotherapy of tumors.

   Hum Gene Ther. 2012 Jul;23(7):700-10. doi: 10.1089/hum.2011.207. Epub 2012 Apr 18.
28.Zha YY, Yang B, Tang ML, Guo QC, Chen JT, Wen LP, Wang M.
   Concentration-dependent effects of fullerenol on cultured hippocampal neuron viability.
   Int J Nanomedicine. 2012;7:3099-109. doi: 10.2147/IJN.S30934. Epub 2012 Jun 29.
29.Wei D, Sun N, Nan G, Wang Y, Liu HQ, Peeters B, Chen ZN, Bian H.
   Construction of recombinant Newcastle disease virus Italien strain for oncolytic
   virotherapy of tumors.

   Hum Gene Ther. 2012 Jul;23(7):700-10. doi: 10.1089/hum.2011.207. Epub 2012 Apr 18.
30.Du Q, Hu B, An HM, Shen KP, Xu L, Deng S, Wei MM.
   Synergistic anticancer effects of curcumin and resveratrol in Hepa1-6 hepatocellular
   carcinoma cells.

   Oncol Rep. 2013 May;29(5):1851-8. doi: 10.3892/or.2013.2310. Epub 2013 Feb 27.
31.Hu Z, Xing Y, Qian Y, Chen X, Tu J, Ren L, Wang K, Chen Z.
   Anti-radiation damage effect of polyethylenimine as a toll-like receptor 5 targeted
   agonist.

   J Radiat Res. 2013 Mar 1;54(2):243-50. doi: 10.1093/jrr/rrs098. Epub 2012 Oct 26.
32.Wang K, Ping S, Huang S, Hu L, Xuan H, Zhang C, Hu F.
   Molecular mechanisms underlying the in vitro anti-inflammatory effects of a
   flavonoid-rich ethanol extract fromchinese propolis (poplar type).

   Evid Based Complement Alternat Med. 2013;2013:127672. doi: 10.1155/2013/127672.
   Epub 2013 Jan 15.
33.Hao Y, Ren J, Liu J, Yang Z, Liu C, Li R, Su Y.
   Immunological changes of chronic oral exposure to depleted uranium in mice.
   Toxicology. 2013 Jul 5;309:81-90. doi: 10.1016/j.tox.2013.04.013. Epub 2013 May 6.
34.Ma JF, Von Kalle M, Plautz Q, -M Xu F, Singh L, Wang L.
   Relaxin promotes in vitro tumour growth, invasion and angiogenesis of human Saos-2
   osteosarcoma cells byAKT/VEGF pathway.

   Eur Rev Med Pharmacol Sci. 2013 May;17(10):1345-50.
35.Shi F, Yang Y, Kouadir M, Fu Y, Yang L, Zhou X, Yin X, Zhao D.
   Inhibition of phagocytosis and lysosomal acidification suppresses neurotoxic prion
   peptide-induced NALP3inflammasome activation in BV2 microglia.

   J Neuroimmunol. 2013 Jul 15;260(1-2):121-5. doi: 10.1016/j.jneuroim.2013.04.016.
   Epub 2013 May 13.
36.Li Z, Gao Q.
   Induction of apoptosis in HT-29 cells by quercetin through mitochondria-mediated
   apoptotic pathway.

   Animal Cells and Systems. Volume 17, Issue 3, 2013.
37.Hao Y, Ren J, Liu C, Li H, Liu J, Yang Z, Li R, Su Y.
   Zinc Protects Human Kidney Cells from Depleted Uranium-induced Apoptosis.
   Basic Clin Pharmacol Toxicol. 2013 Oct 29. doi: 10.1111/bcpt.12167.
38.Chen Z, Zhu M, Pan X, Zhu Y, Yan H, Jiang T, Shen Y, Dong X, Zheng N, Lu J, Ying S,
   Shen Y.
   Inhibition of Hepatitis B virus replication by SAMHD1.
   Biochem Biophys Res Commun. 2014 Aug 8;450(4):1462-8. doi: 10.1016/j.bbrc.2014.07.
   023. Epub 2014 Jul 11.
39.Lu W, Zuo Y, Feng Y, Zhang M.
   SIRT5 facilitates cancer cell growth and drug resistance in non-small cell lung cancer.
   Tumour Biol. 2014 Jul 29.
40.Chen Z, Ye X, Tang N, Shen S, Li Z, Niu X, Lu S, Xu L.
   The histone acetylranseferase hMOF acetylates Nrf2 and regulates anti-drug responses in
   human non-small cell lung cancer.

   Br J Pharmacol. 2014 Jul;171(13):3196-211. doi: 10.1111/bph.12661.
41.Shi JX, Li JS, Hu R, Shi Y, Su X, Guo XJ, Li XM.
   Tristetraprolin is involved in the glucocorticoid-mediated interleukin 8 repression.
   Int Immunopharmacol. 2014 Oct;22(2):480-5. doi: 10.1016/j.intimp.2014.07.031. Epub
   2014 Aug 8.
42.Wang YT, Lu XM, Zhu F, Zhao M.
   The preparation of gold nanoparticles and evaluation of their immunological function
   effects on rats.

   Biomed Mater Eng. 2014;24(1):885-92. doi: 10.3233/BME-130882.
43.Ma X, Zhao T, Ouyang T, Xin S, Ma Y, Chang M.
   Propranolol enhanced adipogenesis instead of induction of apoptosis of hemangiomas stem
   cells.

   Int J Clin Exp Pathol. 2014 Jun 15;7(7):3809-17. eCollection 2014.
44.Yang C, Fan J, Zhuang Z, Fang Y, Zhang Y, Wang S.
   The role of NAD(+)-dependent isocitrate dehydrogenase 3 subunit α in AFB1 induced liver
   lesion.

   Toxicol Lett. 2014 Jan 30;224(3):371-9. doi: 10.1016/j.toxlet.2013.10.037. Epub 2013
   Nov 5.
45.Jiang LM, Nie SP, Zhou HL, Huang DF, Xie MY.
   Carboxymethylation enhances the maturation-inducing activity in dendritic cells of
   polysaccharide from the seeds of Plantago asiatica L.

   Int Immunopharmacol. 2014 Oct;22(2):324-31. doi: 10.1016/j.intimp.2014.06.027. Epub
   2014 Jun 26.
46.Liu Y, Liu H, Zou J, Zhang B, Yuan Z.
   Dengue virus subgenomic RNA induces apoptosis through the Bcl-2-mediated PI3k/Akt
   signaling pathway.

   Virology. 2014 Jan 5;448:15-25. doi: 10.1016/j.virol.2013.09.016. Epub 2013 Oct 16.
47.Liu XD, Zhang FB, Zhou B, Shan H, Chen PY.
   Effect of sonication on different quality parameters of Pinus massoniana pollen.
   Ultrason Sonochem. 2015 Jan;22:174-81. doi: 10.1016/j.ultsonch.2014.07.010. Epub 2014
   Jul 17.



苏ICP备06009238号