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NUPR1基因敲除HEK293T细胞Trizol裂解液
产品编号: L29269
产品包装:500µl
价格: ¥ 1258.00
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产品编号 产品名称 产品包装 产品价格
L29265 NUPR1基因敲除质粒 5µg 999.00元
L29266 NUPR1基因敲除慢病毒 10^8 TU 6999.00元
L29267 NUPR1基因敲除HEK293T细胞 1支/瓶 7999.00元
L29268 NUPR1基因敲除HEK293T细胞RIPA裂解液 100µg 1258.00元
L29269 NUPR1基因敲除HEK293T细胞Trizol裂解液 500µl 1258.00元

NUPR1 Knockout HEK293T Trizol Lysate (NUPR1基因敲除HEK293T细胞Trizol裂解液)是通过同时表达Cas9、目的基因sgRNA和puromycin抗性基因,并实现了目的基因CRISPR敲除的多克隆HEK293T细胞的Trizol裂解液。该细胞中目的基因的敲除已经通过T7EI法的验证。本产品可用于该目的基因敲除后其信号通路相关RNA表达的研究。

本Trizol裂解液源于可同时表达Cas9、目的基因sgRNA和puromycin抗性基因的慢病毒感染HEK293T细胞并经过puromycin筛选后获得的多克隆HEK293T细胞。制备该细胞的相应慢病毒的基因序列的关键图谱信息请参考图1。

图1. 可同时表达sgRNA、Cas9和puromycin抗性的慢病毒其基因序列的关键图谱信息。

该细胞中目的基因的敲除已经通过T7EI法的验证。

由于本细胞是通过CRISPR/Cas9技术获得的多克隆细胞,基于CRISPR/Cas9技术的特点,理论上平均有2/3的细胞发生移码突变而导致了目的基因的敲除,平均有1/3的细胞并未发生移码突变。很多情况下有约2/3的细胞发生目的基因的敲除,已经足以进行很多的目的基因的生物学功能的研究了。如果希望获得100%基因敲除的细胞,可以自行使用本产品筛选单克隆细胞,或者委托碧云天进行单克隆细胞株的筛选服务。

本Trizol裂解液用于实验时,建议同时选购无任何靶向的对照细胞Trizol裂解液Control Knockout HEK293T Trizol Lysate (L00031)或靶向GFP的对照Trizol裂解液GFP Knockout HEK293T Trizol Lysate (L00033)

碧云天同时提供基于CRISPR/Cas9技术的NUPR1基因敲除的质粒(L29265 pLenti-NUPR1-sgRNA)慢病毒(L29266 NUPR1 Knockout Lentivirus)HEK293T细胞(L29267 NUPR1 Knockout HEK293T Cells)HEK293T敲除细胞的RIPA裂解液(L29268 NUPR1 Knockout HEK293T RIPA Lysate)HEK293T敲除细胞的Trizol裂解液(L29269 NUPR1 Knockout HEK293T Trizol Lysate)等产品,具体请在碧云天网站查询或在本产品网页点击相应产品。

NUPR1基因的基本信息如下:

Species Gene Symbol Gene ID GenBank Accession Transcript
Human NUPR1 26471 BC002434 NM_012385

 

About the gene
Official Symbol NUPR1
Previous Symbol -
Official Full Name nuclear protein 1, transcriptional regulator
Synonyms COM1; p8
Location 16p11.2
Gene Type protein-coding gene
Uniprot ID O60356
Pathway/Library others
Gene Summary Transcription regulator that converts stress signals into a program of gene expression that empowers cells with resistance to the stress induced by a change in their microenvironment. Thereby participates in regulation of many process namely cell-cycle, apoptosis, autophagy and DNA repair responses (PubMed:16478804, PubMed:19650074, PubMed:16300740, PubMed:19723804, PubMed:11056169, PubMed:22858377, PubMed:11940591, PubMed:18690848, PubMed:22565310, PubMed:20181828, PubMed:30451898). Controls cell cycle progression and protects cells from genotoxic stress induced by doxorubicin through the complex formation with TP53 and EP300 that binds CDKN1A promoter leading to transcriptional induction of CDKN1A (PubMed:18690848). Protects pancreatic cancer cells from stress-induced cell death by binding the RELB promoter and activating its transcription, leading to IER3 transactivation (PubMed:22565310). Negatively regulates apoptosis through interaction with PTMA (PubMed:16478804). Inhibits autophagy-induced apoptosis in cardiac cells through FOXO3 interaction, inducing cytoplasmic translocation of FOXO3 thereby preventing the FOXO3 association with the pro-autophagic BNIP3 promoter (PubMed:20181828). Inhibits cell growth and facilitates programmed cell death by apoptosis after adriamycin-induced DNA damage through transactivation of TP53 (By similarity). Regulates methamphetamine-induced apoptosis and autophagy through DDIT3-mediated endoplasmic reticulum stress pathway (By similarity). Participates in DNA repair following gamma-irradiation by facilitating DNA access of the transcription machinery through interaction with MSL1 leading to inhibition of histone H4' Lys-16' acetylation (H4K16ac) (PubMed:19650074). Coactivator of PAX2 transcription factor activity, both by recruiting EP300 to increase PAX2 transcription factor activity and by binding PAXIP1 to suppress PAXIP1-induced inhibition on PAX2 (PubMed:11940591). Positively regulates cell cycle progression through interaction with COPS5 inducing cytoplasmic translocation of CDKN1B leading to the CDKN1B degradation (PubMed:16300740). Coordinates, through its interaction with EP300, the assiociation of MYOD1, EP300 and DDX5 to the MYOG promoter, leading to inhibition of cell-cycle progression and myogenic differentiation promotion (PubMed:19723804). Negatively regulates beta cell proliferation via inhibition of cell-cycle regulatory genes expression through the suppression of their promoter activities (By similarity). Also required for LHB expression and ovarian maturation (By similarity). Exacerbates CNS inflammation and demyelination upon cuprizone treatment (By similarity). NUPR1_HUMAN,O60356
包装清单:
产品编号 产品名称 包装
L29269 NUPR1 Knockout HEK293T Trizol Lysate 500µl
说明书 1份
保存条件:

-20℃保存,至少6个月有效;-80℃保存,至少一年有效。

注意事项:

碧云天拥有sgRNA序列的知识产权,如果需要sgRNA序列,请在订购后发送邮件向info@beyotime.com索取。sgRNA序列信息与本产品,未经碧云天书面许可不得用于任何商业用途,也不得移交给订货人所在实验室外的任何个人或单位。使用者在发表研究论文或结果时,应注明来源。

对于非目录产品的CRISPR基因敲除细胞Trizol裂解液的定制,可联系碧云天技术服务service@beyotime.com

本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 本细胞Trizol裂解液中含有不少于1×106个细胞。
2. 按照碧云天的Trizol (总RNA抽提试剂) (R0016)中的使用说明进行RNA的抽提:https://www.beyotime.com/product/R0016.htm;或按照Invitrogen™的TRIzol™ Reagent的使用说明进行RNA的抽提,后续可以用于反转录、qRT-PCR、NGS等各种常见用途。
相关产品:
产品编号 产品名称 包装
L00031 Control Knockout HEK293T Trizol Lysate 500µl
L00033 GFP Knockout HEK293T Trizol Lysate 500µl
D7166 BeyoRT™ cDNA第一链合成试剂盒(RNase H-) 10次
D7168 BeyoRT™ II cDNA第一链合成试剂盒(RNase H-) 20/100/500次
D7170 BeyoRT™ II cDNA合成试剂盒(with gDNA Eraser) 20/100/500次
D7178 BeyoRT™ III cDNA第一链合成试剂盒 20/100/500次
D7180 BeyoRT™ III cDNA合成试剂盒 (with gDNA EZeraser) 20/100/500次
D7182 BeyoRT™ III cDNA第一链合成预混液(5X) 20/100/500次
D7185 BeyoRT™ III cDNA合成预混液(5X)(with gDNA EZeraser) 20/100/500次
D7260 BeyoFast™ SYBR Green qPCR Mix (2X) 1/5/25ml
D7262 BeyoFast™ SYBR Green qPCR Mix (2X, Low ROX) 1/5/25ml
D7265 BeyoFast™ SYBR Green qPCR Mix (2X, High ROX) 1/5/25ml
D7268 BeyoFast™ SYBR Green One-Step qRT-PCR Kit 100/500次
D7271 BeyoFast™ Probe qPCR Mix (2X) 1/5/25ml
D7272 BeyoFast™ Probe qPCR Mix (2X, Low ROX) 1/5/25ml
D7273 BeyoFast™ Probe qPCR Mix (2X, High ROX) 1/5/25ml
D7277 BeyoFast™ Probe One-Step qRT-PCR Kit 100/500次
R0011 Beyozol (总RNA抽提试剂) 100ml
R0016 Trizol (总RNA抽提试剂) 100ml
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