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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| L30215 | PSME1基因敲除质粒 | 5µg | 999.00元 |
| L30216 | PSME1基因敲除慢病毒 | 10^8 TU | 6999.00元 |
| L30217 | PSME1基因敲除HEK293T细胞 | 1支/瓶 | 7999.00元 |
| L30218 | PSME1基因敲除HEK293T细胞RIPA裂解液 | 100µg | 1258.00元 |
| L30219 | PSME1基因敲除HEK293T细胞Trizol裂解液 | 500µl | 1258.00元 |
PSME1 Knockout Lentivirus(PSME1基因敲除慢病毒)是一种感染动物细胞后可以同时表达Cas9、目的基因sgRNA和puromycin抗性基因的慢病毒。本产品用于在动物细胞中基于CRISPR/Cas9技术敲除目的基因，并且本慢病毒中sgRNA的有效性已经通过T7EI法的验证。
本慢病毒基因序列的关键图谱信息请参考图1。本慢病毒可用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。
图1. 可同时表达sgRNA、Cas9和puromycin抗性的本慢病毒其基因序列的关键图谱信息。
用于包装本慢病毒的质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。
本慢病毒用于实验时，建议同时选购无任何靶向的对照慢病毒Control Knockout Lentivirus (L00015)或靶向GFP的对照慢病毒GFP Knockout Lentivirus (L00017)。
碧云天同时提供基于CRISPR/Cas9技术的PSME1基因敲除的质粒(L30215 pLenti-PSME1-sgRNA)、慢病毒(L30216 PSME1 Knockout Lentivirus)、HEK293T细胞(L30217 PSME1 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L30218 PSME1 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L30219 PSME1 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。
PSME1基因的基本信息如下：
| Species | Gene Symbol | Gene ID | GenBank Accession | Transcript |
| Human | PSME1 | 5720 | BC000352 | NM_006263 |
| About the gene | |
| Official Symbol | PSME1 |
| Previous Symbol | - |
| Official Full Name | proteasome activator subunit 1 |
| Synonyms | IFI5111; PA28alpha |
| Location | 14q12 |
| Gene Type | protein-coding gene |
| Uniprot ID | Q06323 |
| Pathway/Library | others |
| Gene Summary | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified. |
| 产品编号 | 产品名称 | 包装 |
| L30216 | PSME1 Knockout Lentivirus | 108 TU |
| — | 说明书 | 1份 |
-80℃保存，至少一年有效。
注意事项：碧云天拥有sgRNA序列的知识产权，如果需要sgRNA序列，请在订购后发送邮件向info@beyotime.com索取。sgRNA序列信息与本慢病毒，未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何个人或单位。使用者在发表研究论文或结果时，应注明来源。
对于非目录产品的CRISPR基因敲除用的慢病毒的定制，可联系碧云天技术服务service@beyotime.com。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| 产品编号 | 产品名称 | 包装 |
| L00015 | Control Knockout Lentivirus | 108 TU |
| L00017 | GFP Knockout Lentivirus | 108 TU |
| C0222 | 青霉素-链霉素溶液(100X) | 100ml |
| C0351-1ml | Polybrene (Hexadimethrine Bromide) | 1ml |
| C0351-50mg | Polybrene (Hexadimethrine Bromide) | 50mg |
| D0508S/M | 基因组编辑突变检测试剂盒 | 25/100次 |
| D7080S/M/L | T7 Endonuclease I (CRISPR等基因突变鉴定用) | 250/1250/5000U |
| ST551-10mg | Puromycin Dihydrochloride (嘌呤霉素) | 10mg/ml×1ml |
| ST551-50mg | Puromycin Dihydrochloride (嘌呤霉素) | 10mg/ml×5ml |
| ST551-250mg | Puromycin Dihydrochloride (嘌呤霉素) | 250mg |
| ST1380-500mg | Polybrene (≥94%, Reagent grade) | 500mg |
| ST1380-2g | Polybrene (≥94%, Reagent grade) | 2g |
| ST1380-10g | Polybrene (≥94%, Reagent grade) | 10g |
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