使用说明：
1. 取出Griess Reagent I和II，使回复室温。
2. 用待测样品所用溶液稀释标准品(1-100μM)。
    例如样品为细胞培养液上清，细胞培养液为DMEM＋10%FBS，则用DMEM＋10%FBS稀释标准品。通常标准品
    的浓度可取0, 1, 2, 5, 10, 20, 40, 60, 100μM。
3. 按50μl/孔，在96孔板中加入标准品及样品。
    样品为培养液上清，可以直接取样，如果有可沉淀物则需离心后取上清。如样品为细胞或组织，可以快
    速冻融裂解，然后离心沉淀取上清，体积不足50μl可以用重蒸水或0.9%NaCl稀释(相应地标准品也需用
    重蒸水或0.9%NaCl稀释)。细胞或组织也可以用用于Western或IP的裂解液(无需添加抑制剂)裂解，同样
    标准品也需相应稀释。碧云天生产的Western及IP细胞裂解液(P0013)或各种RIPA裂解液裂解细胞后适用
    于一氧化氮的测定。
4. 按50μl/孔，在各孔中加入室温Griess Reagent I。
5. 按50μl/孔，各孔中加入室温Griess Reagent II。
6. 540nm 测定吸光度。
    如无540nm滤光片，520-560nm的滤光片也可。如无酶标仪或合适的滤光片，也可以通过目测比色，确定
    样品中一氧化氮的浓度。目测比色时标准品需要更为精细的浓度梯度。

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