|
辣根过氧化物酶标记山羊抗小鼠IgG(H+L) |
产品编号: A0216
产品包装: 1ml
产品价格: 191.00元 |
|
|
|
产品简介
产品简介:
来源 |
用途 |
WB |
ELISA |
IHC |
Goat |
WB, ELISA, IHC |
1:1000 |
1:250 |
1:50 |
WB, Western blot; ELISA, Enzyme-linked Immunosorbent Assay; IHC, Immunohistochemistry.
本辣根过氧化物酶标记山羊抗小鼠IgG(H+L)(HRP-labeled Goat Anti-Mouse IgG(H+L))为进口分装,用于Western、ELISA和IHC等的二抗。HRP,即horseradish peroxidase,中文名为辣根过氧化物酶,可以在Western检测时催化ECL类试剂(如ECL、BeyoECL等)产生化学发光,可以在ELISA时催化TMB产生蓝色,也可以在IHC或Western blot检测时催化DAB产生棕色沉淀。
本辣根过氧化物酶标记山羊抗小鼠IgG(H+L)用于WB、ELISA和IHC的推荐稀释比例参考上表,实际实验操作过程中需根据抗原和抗体的具体情况适当调节二抗的稀释比例。对于WB,推荐的稀释范围为1:500-2000。
本抗体为用纯化的小鼠IgG免疫山羊,然后用亲和纯化柱对获得的抗血清进行纯化,并经过人IgG吸附纯化的优质二抗。对人IgG几乎没有结合能力。
本抗体如果用于常规的Western检测,以每次检测需10毫升1:1000稀释的二抗计,至少可以检测100次。
包装清单:
产品编号 |
产品名称 |
包装 |
A0216 |
辣根过氧化物酶标记山羊抗小鼠IgG(H+L) |
1ml |
— |
说明书 |
1份 |
保存条件:
-20℃保存,一年有效。4℃保存,半年有效。
注意事项:
叠氮钠会抑制辣根过氧化物酶的活性,在含有辣根过氧化物酶的二抗中不可加入叠氮钠。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普 通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
使用说明
使用说明:
1. Western、ELISA或IHC请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀
释。
产品图片
相关产品
相关论文
使用本产品的文献:
1. Wang YX, Xu XY, Su WL, Wang Q, Zhu WX, Chen F, Jin G, Liu YJ, Li YD, Sun YP, Gao
WC, Ruan CP.
Activation and Clinical Significance of p38 MAPK Signaling Pathway in Patients With
Severe Trauma.
J Surg Res. 2008 Dec 3.
2. He J, Sun H, Zhang D, Sun Y, Ma L, Chen L, Liu Z, Xiong C, Yan G, Zhu C.
Cloning and characterization of 60S ribosomal protein L22 (RPL22) from Culex pipiens
pallens.
Comp Biochem Physiol B Biochem Mol Biol. 2009 Jun;153(2):216-22.
3. Hua W, Jiang J, Rong X, Wu R, Qiu H, Zhang Y, Chen Q.
The dual role of the cystathionine gamma-lyase/hydrogen sulfide pathway in CVB3-induced
myocarditis in mice.
Biochem Biophys Res Commun. 2009 Oct 23;388(3):595-600. Epub 2009 Aug 18.
4. Gong F, Cheng X, Wang S, Zhao Y, Gao Y, Cai H.
Heparin-immobilized polymers as non-inflammatory and non-thrombogenic coating materials
for arsenic trioxide eluting stents.
Acta Biomater. 2010 Feb;6(2):534-46. Epub 2009 Jul 14.
5. Yuan D, Pan Y, Zhang J, Shao C.
Role of nuclear factor-kappaB and P53 in radioadaptive response in Chang live cells.
Mutat Res. 2010 Jun 1;688(1-2):66-71. Epub 2010 Mar 20.
6. Zeng J, Wang Q, Lin J, Deng K, Zhao X, Tang D, Liu X.
Arabidopsis cryptochrome-1 restrains lateral roots growth by inhibiting auxin transport.
J Plant Physiol. 2010;167(8):670-3. Epub 2010 Feb 4.
7. Wu J, Hu CP, Gu QH, Li YP, Song M.
Trichostatin A sensitizes cisplatin-resistant A549 cells to apoptosis by up-regulating
death-associated protein kinase.
Acta Pharmacol Sin. 2010;31(1):93-101.
8. Wu YF, Yang LJ, Zhong LW.
Decreased serum levels of thioredoxin in patients with coronary artery disease
plus
hyperhomocysteinemia is strongly associated with the disease severity
Atherosclerosis.2010.
9. Cao BY, Yang YP, Luo WF, Mao CJ, Han R, Sun X, Cheng J, Liu CF.
Paeoniflorin, a potent natural compound, protects PC12 cells from MPP+ and acidic damage
via autophagic pathway.
J Ethnopharmacol. 2010;131(1):122-9. Epub 2010 Jun 15.
10.Xu S, Zhou Z, Zhang L, Yu Z, Zhang W, Wang Y, Wang X, Li M, Chen Y, Chen C, He M, Zhang
G, Zhong M.
Exposure to 1800 MHz radiofrequency radiation induces oxidative damage to
mitochondrial DNA in primary cultured neurons.
Brain Res. 2010;1311:189-96. Epub 2009 Oct 30.
11.Zhao P, Tang SM, Liu T, Qin GX, Guo XJ
Expression and Activity Analysis of Non-Structural Protein 2 (NS2) of Bombyx mori
Densovirus Zhenjiang Strain
Volume 9, Issue 12, December 2010, Pages 1821–1828
12.Yin Z, Wang L, Xiang Y, Ruan Y, Li J, Wang X, Ichim TE, Chen S, Chen G.
Resistance of neonatal porcine Sertoli cells to human xenoantibody and
complement-mediatedlysis is associated with low expression of alpha-Gal and high
production of clusterin and CD59.
Xenotransplantation. 2010 May-Jun;17(3):215-23.
13.Wang X, Yang Q, Wang P, Luo L, Chen Z, Liao B, Li G.
Derp2-mutant gene vaccine inhibits airway inflammation and up-regulates Toll-like
receptor9 in anallergic asthmatic mouse model.
Asian Pac J Allergy Immunol. 2010 Dec;28(4):287-93.
14.Wang J, Ruan K.
miR-335 is involved in the rat epididymal development by targeting the mRNA of RASA1.
Biochem Biophys Res Commun. 2010 Nov 12;402(2):222-7.
15.Song L, Ma YP, Liu ZG,
Experimental study on the relationship between levodopa-induced dyskinesia and
phosphorylation of ERK in the striatum
CJCNN, 2011.11(1)
16.崔冉亮 戎凯 吕朴 胡海燕 褚玉晶 丁楠 邓艳秋
四氧嘧啶脑室内注射对小鼠学习记忆的影响
中华神经医学杂志,2011.4,346-350
17.陈 妮, 左国伟, 潘 玥, 赖国旗,,杨根岭, 向廷秀, 韩建红, 黄 云
小鼠CHD5基因siRNA重组腺病毒载体的构建及鉴定
西南大学学报,2011,33(8)
18.Zeng HC, Zhang L, Li YY, Wang YJ, Xia W, Lin Y, Wei J, Xu SQ
Inflammation-like glial response in rat brain induced by prenatal PFOS exposure.
Neurotoxicology. 2011 Jan;32(1):130-9. Epub 2010 Oct 19.
19.Cai G, Jiang M, Zhou Y, Gu X, Zhang B, Zou M, Zhou X, Bao J, Cao G, Zhang R
Generation of a liver targeting fusion interferon and its bioactivity analysis in vitro
Pharmazie. 2011 Oct;66(10):761-5
20.Feng Y, Yang Q, Xu J, Qian G, Wang Y, Liu Y, Duan G.
Preparation and identification ofthe lipopolysaccharide binding protein mimic epitope
peptidevaccine that prevents endotoxin-induced acute lung injury in mice.
Vaccine. 2011 May 31;29(24):4162-72. Epub 2011 Apr 5
21.Longxi P, Buwu F, Yuan W, Sinan G (2011)
Expression of p53 in the Effects of Artesunate on Induction of Apoptosis and Inhibition
of Proliferation in Rat Primary Hepatic Stellate Cells.
PLoS one,2011, 6(10): e26500.
22.Wang T, Zhang L, Shi C, Sun H, Wang J, Li R, Zou Z, Ran X, Su Y
TGF-β-induced miR-21 negatively regulates the antiproliferative activity but has no
effect on EMTof TGF-β in HaCaT cells.
Int J Biochem Cell Biol. 2012 Feb;44(2):366-76.doi:10.1016/j.biocel.2011.11.012.
Epub 2011 Nov 22.
23.Tang R, Yang C, Tao JL, You YK, An N, Li SM, Wu HL, Liu HF.
Epithelial-mesenchymal transdifferentiation of renal tubular epithelial cells induced by
urinaryproteins requires the activation of PKC-α and βI isozymes.
Cell Biol Int. 2011 Sep;35(9):953-9
24.Wang WB, Feng LX, Yue QX, Wu WY, Guan SH, Jiang BH, Yang M, Liu X, Guo DA
Paraptosis accompanied by autophagy and apoptosis was induced by celastrol, a
naturalcompound with influence on proteasome, ER stress and Hsp90.
J Cell Physiol. 2012 May;227(5):2196-206.
25.Feng DD, Yang W, Yang WJ, Yu QY, Zhang Z.
Molecular cloning, heterogenous expression and theinduction profiles after
organophosphatephoxim exposure of the carboxylesterase Bmae33 in the silkworm,
Bombyx mori.
African Journal of Biotechnology Vol. 11(42), pp. 9915-9923, 24 May,2012/6/12
26.Wang XJ,Wei DL,Song ZG,Jiao HC Lin H
Effects of Fatty Acid Treatments on the Dexamethasone-Induced Intramuscular Lipid
Accumulation in Chickens.
PLoS One. 2012;7(5):e36663. doi: 10.1371/journal.pone.0036663. Epub 2012 May 18.
27.Wu X, Zhao B, Li W, Chen Y, Liang R, Li L, Jin Y, Ruan K
MiR-200a is involved in rat epididymal development by targeting β-catenin mRNA.
Acta Biochim Biophys Sin (Shanghai).2012 Mar;44(3):233-40.doi:10.1093/abbs/gmr122.
Epub 2012 Jan 11.
28.Yu L, Lu M, Wang P, Chen X.
Trichostatin A Ameliorates Myocardial Ischemia/Reperfusion Injury Through Inhibition
ofEndoplasmic Reticulum Stress-induced Apoptosis.
Arch Med Res. 2012 Apr;43(3):190-6. doi: 10.1016/j.arcmed.2012.04.007.
Epub 2012 May 4.
29.Su H, Ma C, Liu J, Li N, Gao M, Huang A, Wang X, Huang W, Huang X.
Downregulation of nuclear receptor FXR is associated with multiple malignant
clinicopathological characteristicsin human hepatocellular carcinoma.
Am J Physiol Gastrointest Liver Physiol. 2012 Dec 1;303(11):G1245-53.
doi: 10.1152/ajpgi.00439.2011. Epub 2012 Oct 4.
30.Fauzee NJ, Wang YL, Dong Z, Li QG, Wang T, Mandarry MT, Xu L, Pan J.
Novel hydrophilic docetaxel (CQMU-0519) analogue inhibits proliferation and induces
apoptosis in human A549 lung, SKVO3 ovarian and MCF7 breast carcinoma cell lines.
Cell Prolif. 2012 Aug;45(4):352-64. doi: 10.1111/j.1365-2184.2012.00825.x.
Epub 2012 Jun 5.
31.Ling C, Chen G, Chen G, Zhang Z, Cao B, Han K, Yin J, Chu A, Zhao Y, Mao X.
A deuterated analog of dasatinib disrupts cell cycle progression and displays
anti-non-small cell lung canceractivity in vitro and in vivo.
Int J Cancer. 2012 Nov 15;131(10):2411-9. doi: 10.1002/ijc.27504. Epub 2012 Mar 28.
32.Song Z, Liu L, Yue Y, Jiao H, Lin H, Sheikhahmadi A, Everaert N, Decuypere E, Buyse J.
Fasting alters protein expression of AMP-activated protein kinase in the hypothalamus
of broiler chicks (Gallusgallus domesticus).
Gen Comp Endocrinol. 2012 Sep 15;178(3):546-55. doi: 10.1016/j.ygcen.2012.06.026.
Epub 2012 Jul 5.
33.Wang XJ, Song ZG, Jiao HC, Lin H.
Dexamethasone facilitates lipid accumulation in chicken skeletal muscle.
Stress. 2012 Jul;15(4):443-56. doi: 10.3109/10253890.2011.639413. Epub 2011 Dec 7.
34.Xie J, Wang C, Yang A, Zhang B, Yin Q, Huang H, Chen M.
Cucurmosin Kills Human Pancreatic Cancer SW-1990 Cells in vitro and in vivo.
Anticancer Agents Med Chem. 2013 Jul 1;13(6):952-6.
35.Zhang H, Chen W, Duan CJ, Zhang CF.
Overexpression of HSPA2 is correlated with poor prognosis in esophageal squamous cell
carcinoma.
World J Surg Oncol. 2013 Jun 18;11(1):141.
36.Li T, Zhu J, Ma K, Liu N, Feng K, Li X, Wang S, Bie P.
Autologous bone marrow-derived mesenchymal stem cell transplantation promotes liver
regeneration after portal vein embolization in cirrhotic rats
Journal of Surgical Research.Available online 16 May 2013.
37.Liu F, Xie M, Chen D, Li J, Ding W.
Effect of V(IV)O(dipic-Cl)(H2O)2 on Lipid Metabolism Disorders in the Liver of
STZ-Induced Diabetic Rats.
J Diabetes Res. 2013;2013:956737. doi: 10.1155/2013/956737. Epub 2013 Mar 20.
38.Zhang H, Tang L, Chen S, Yang Y, Chen M, Luo J.
Effect of advanced glycation end products on the expression of hypoxia-inducible
factor-1α and vascularendothelial growth factor proteins in RF/6A cells.
Exp Ther Med. 2013 May;5(5):1519-1522. Epub 2013 Mar 15.
39.Zhou CH, Lin-Li, Zhu XY, Wen-Tang, Hu DM, Dong Y, Li LY, Wang SF.
Protective effects of edaravone on experimental chronic pancreatitis induced by
dibutyltin dichloride in rats.
Pancreatology. 2013 Mar-Apr;13(2):125-32. doi: 10.1016/j.pan.2013.01.007.
Epub 2013 Feb 6.
40.Jin XL, Sun QS, Liu F, Yang HW, Liu M, Liu HX, Xu W, Jiang YY.
microRNA 21-mediated suppression of Sprouty1 by Pokemon affects liver cancer cell
growth and proliferation.
J Cell Biochem. 2013 Jul;114(7):1625-33. doi: 10.1002/jcb.24504.
41.Su L, Woodard RW, Chen J, Wu J.
Extracellular Location of Thermobifida fusca Cutinase Expressed in Escherichia coli
BL21(DE3) without Mediationof a Signal Peptide.
Appl Environ Microbiol. 2013 Jul;79(14):4192-8. doi: 10.1128/AEM.00239-13.
Epub 2013 Apr 19.
42.Zhan C, Shi Y, Lu C, Wang Q.
Pyruvate kinase M2 is highly correlated with the differentiation and the prognosis of
esophageal squamous cellcancer.
Dis Esophagus. 2013 Jan 14. doi: 10.1111/dote.12023.
43.Tang C, Yang L, Jiang X, Xu C, Wang M, Wang Q, Zhou Z, Xiang Z, Cui H.
Antibiotic drug tigecycline inhibited cell proliferation and induced autophagy in
gastric cancer cells.
Biochem Biophys Res Commun. 2014 Mar 28;446(1):105-12. doi: 10.1016/j.bbrc.
2014.02.043. Epub 2014 Feb 28.
44.Li ZH, Zhang Q, Wang HB, Zhang YN, Ding D, Pan LQ, Miao D, Xu S, Zhang C, Luo PH,
Naranmandura H, Chen SQ.
Preclinical studies of targeted therapies for CD20-positive B lymphoid malignancies by
Ofatumumab conjugated with auristatin.
Invest New Drugs. 2014 Feb;32(1):75-86. doi: 10.1007/s10637-013-9995-y.
Epub 2013 Aug 1.
45.Ren T, Qiu Y, Wu W, Feng X, Ye S, Wang Z, Tian T, He Y, Yu C, Zhou Y.
Activation of adenosine A3 receptor alleviates TNF-α-induced inflammation through
inhibition of the NF-κB signaling pathway in human colonic epithelial cells.
Mediators Inflamm. 2014;2014:818251. doi: 10.1155/2014/818251. Epub 2014 Apr 22.
46.Zhu Y, Zhang JJ, Liang WB, Zhu R, Wang B, Miao Y, Xu ZK.
Pancreatic cancer counterattack: MUC4 mediates Fas-independent apoptosis of antigen-
specific cytotoxic T lymphocyte.
Oncol Rep. 2014 Apr;31(4):1768-76. doi: 10.3892/or.2014.3016. Epub 2014 Feb 10.
47.Khan MK, He L, Zhang W, Wang Y, Tao Q, Song Q, Sajid MS, Yu Q, Hu J, Fang R, Hu M,
Zhou Y, Zhao J.
Identification of two novel HSP90 proteins in Babesia orientalis: molecular
characterization, and computational analyses of their structure, function, antigenicity
and inhibitor interaction.
Parasit Vectors. 2014 Jun 26;7:293. doi: 10.1186/1756-3305-7-293.
48.Ruan Z, Liu G, Guo Y, Zhou Y, Wang Q, Chang Y, Wang B, Zheng J, Zhang L.
First report of a thioredoxin homologue in jellyfish: molecular cloning, expression and
antioxidant activity of CcTrx1 from Cyanea capillata.
PLoS One. 2014 May 13;9(5):e97509. doi: 10.1371/journal.pone.0097509. eCollection
2014.
|