使用说明：
1.Western检测：
  A.按照1:500用碧云天提供的Western一抗稀释液稀释抗体。
  B.把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动1-2小时，确保稀释的抗体至少能
    在摇动的瞬间覆盖蛋白膜。
  C.回收稀释的一抗，4℃保存以备下次继续使用。
  D.按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网
    页：http://www.beyotime.com/western.htm
2.免疫染色：
   可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作
   可以参考如下网页： http://www.beyotime.com/immunol-staining.htm
3.其它实验操作请自行参考适当的protocol进行。

使用本产品的文献：
1. Wang MZ, Tian WR, Tian ZJ, Qu PP, Gao SS, Zhang ZH, Zhang QY, Hou CX and Li HH.
   Effect of RNA Interference Hsp72 Gene Expression on Development of Mouse Preimplantation
   Embryos.
   Agricultural Sciences in China. 2009, 8(2): 223-230.
2. Liu YL, Zhao Y, Dai ZM, Chen HM, Yang WJ.
   Formation of diapause cyst shell in brine shrimp, Artemia parthenogenetica, and its
   resistance role in environmental stresses.
   J Biol Chem. 2009 Jun 19;284(25):16931-8. Epub 2009 Apr 24.
3. Zhang Z, Wang Q, Li P, Zhou Y, Li S, Yi W, Chen A, Kong P, Hu C.
   Overexpression of the Interferon regulatory factor 4-binding protein in human colorectal
   cancer and its clinical significance.
   Cancer Epidemiol. 2009 Aug;33(2):130-6. Epub 2009 Jul 10.
4. Hua W, Jiang J, Rong X, Wu R, Qiu H, Zhang Y, Chen Q.
   The dual role of the cystathionine gamma-lyase/hydrogen sulfide pathway in CVB3-induced
   myocarditis in mice.
   Biochem Biophys Res Commun. 2009 Oct 23;388(3):595-600. Epub 2009 Aug 18.
5. Wei CJ, Tian XF, Adam RD, Lu SQ.
   Giardia lamblia: intracellular localization of alpha8-giardin.
   Exp Parasitol. 2010;126(4):489-96. Epub 2010 May 31.
6. Li L, Cao Z, Jia P, Wang Z.
   Calcium signals and caspase-12 participated in paraoxon-induced apoptosis in EL4 cells.
   Toxicol In Vitro. 2010;24(3):728-36. Epub 2010 Jan 15.
7. Hong Y, Han H, Peng J, Li Y, Shi Y, Fu Z, Liu J, Lin J, Li X.
   Schistosoma japonicum: cloning, expression and characterization of a gene encoding the
   α5-subunit of the proteasome.
   Exp Parasitol. 2010;126(4):517-25. Epub 2010 Jul 3.
8. Fei E, Ma X, Zhu C, Xue T, Yan J, Xu Y, Zhou J, Wang G.
   Nucleocytoplasmic shuttling of dysbindin-1, a schizophrenia-related protein, regulates
   synapsin Iexpression.
   J Biol Chem. 2010 Dec 3;285(49):38630-40.
9. Wu L, Zhao Q, Zhu X, Peng M, Jia C, Wu W, Zheng J, Wu XZ
   A novel function of microRNA let-7d in regulation of galectin-3 expression in attention
   deficithyperactivity disorder rat brain.
   Brain Pathol. 2010 Nov;20(6):1042-54.
10.Li B, Xu W, Xu Y, Zhang Y, Wang T, Bai Y, Han C, Zhang A, Xu Z, Chong K.
   Integrative study on proteomics, molecular physiology, and genetics reveals an
   accumulation ofcyclophilin-like protein, TaCYP20-2, leading to an increase of Rht
   protein and dwarf in a novel GA-insensitive mutant (gaid) in Wheat.
   J Proteome Res. 2010 Aug 6;9(8):4242-53.
11.Li G, Shi Y, Huang H, Zhang Y, Wu K, Luo J, Sun Y, Lu J, Benovic JL, Zhou N
   Internalization of the human nicotinic acid receptor GPR109A is regulated by G(i),
   GRK2, andarrestin3.
   J Biol Chem. 2010 Jul 16;285(29):22605-18.
12.Yin Z, Wang L, Xiang Y, Ruan Y, Li J, Wang X, Ichim TE, Chen S, Chen G.
   Resistance of neonatal porcine Sertoli cells to human xenoantibody and
   complement-mediatedlysis is associated with low expression of alpha-Gal and high
   production of clusterin and CD59.
   Xenotransplantation. 2010 May-Jun;17(3):215-23.
13.Tang WJ, Wang LF, Xu XY, Zhou Y, Jin WF, Wang HF, Gao J.
   Autocrine/paracrine action of vitamin D on FGF23 expression in cultured rat osteoblasts.
   Calcif Tissue Int. 2010 May;86(5):404-10.
14.张 霞，曹荣峰，丛 霞，李金波，孙 琪，高善颂，田文儒
   CaM 对小鼠胎儿成纤维细胞 Hsp70 表达的影响
   中国农业科学，2011，44（7）
15.Yang F, Wang MR, Ma YG, Ma WM, Yang WJ.
   Prawn lipocalin: characterization of a color shift induced by gene knockdown and ligand
   bindingassay.
   J Exp Zool A Ecol Genet Physiol. 2011 Nov 1;315(9):562-71.
16.Zou X, Jin YM, Liu PP, Wu QJ, Liu JM, Lin JJ
   RNAi silencing of calcium-regulated heat-stable protein of 24 kDa in Schistosoma
   japonicumaffects parasite growth.
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   Distinct kinetic and spatial patterns of protein kinase C (PKC)- and epidermal growth
   factorreceptor (EGFR)-dependent activation of extracellular signal-regulated kinases 1
   and 2 by humannicotinic acid receptor GPR109A.
   J Biol Chem. 2011 Sep 9;286(36):31199-212. Epub 2011 Jul 18.
18.Dai L, Chen DF, Liu YL, Zhao Y, Yang F, Yang JS, Yang WJ.
   Extracellular matrix peptides of Artemia cyst shell participate in protecting encysted
   embryosfrom extreme environments.
   PLoS One. 2011;6(6):e20187. Epub 2011 Jun 6.
19.Li J, Jiang J, Qian Q, Xu Y, Zhang C, Xiao J, Du C, Luo W, Zou G, Chen M, Huang Y,
   Feng Y, Cheng Z, Yuan M, Chong K.
   Mutation of rice BC12/GDD1, which encodes a kinesin-like protein that binds to a GA
   biosynthesis gene promoter, leads to dwarfism with impaired cell elongation.
   Plant Cell. 2011 Feb;23(2):628-40. Epub 2011 Feb 15.
20.Ma L, Xu H, Zhu J, Ma S, Liu Y, Jiang RJ, Xia Q, Li S.
   Ras1(CA) overexpression inthe posterior silk gland improves silk yield.
   Cell Res. 2011 Jun;21(6):934-43.
21.Li Q, Li J, Lan H, Wang N, Hu X, Chen L, Li N
   Effects of fasting and refeeding on expression of MAFbx and MuRF1 in chick skeletal
   muscle.
   Sci China Life Sci. 2011 Oct;54(10):904-7.
22.Li W, Han Y, Tao F, Chong K.
   Knockdown of SAMS genes encoding S-adenosyl-l-methionine synthetases causes
   methylationalterations of DNAs and histones and leads to late flowering in rice.
   J Plant Physiol. 2011 Oct 15;168(15):1837-43. Epub 2011 Jul 14.
23.Xie Y, Zhang J, Xu Y, Shao C.
   SirT1 confers hypoxia-induced radioresistance via the modulation of c-Myc stabilization
   onhepatoma cells.
   J Radiat Res. 2012;53(1):44-50
24.Mao J, Li X, Chen W, Xu B, Zhang H, Li H, Wang L, Jin X, Zhu J, Lin G, Wang W, Chen L
   Cell cycle-dependent subcellular distribution of ClC-3 in HeLa cells.
   Histochem Cell Biol. 2012 Jun;137(6):763-76.doi: 10.1007/s00418-012-0937-0.
   Epub 2012 Feb 28.
25.Muhammad Khan,Bin Zheng,Fei Yi,Azhar Rasul,Zhuyi Gu
   Pseudolaric Acid B Induces Caspase-Dependent and Caspase-Independent Apoptosis in U87
   Glioblastoma Cells
   Evidence-Based Complementary and Alternative Medicine,2012, 11 pages
26.Yang Y, Jin Y, Liu P, Shi Y, Cao Y, Liu J, Shi Y, Li H, Lin J
   RNAi silencing of type V collagen in Schistosoma japonicum affects parasite
   morphology,spawning, and hatching.
   Parasitol Res. 2012 May 26.
27.Wang J, Wu Z, Jin Q
   COPI Is Required for Enterovirus 71 Replication.
   PLoS One. 2012;7(5):e38035.doi: 10.1371/journal.pone.0038035. Epub 2012 May 25.
28.He M, Ye S, Ren R, Dong C, Xie Y, Yuan D, Shao C.
   Cytochrome-c mediated a bystander response dependent on inducible nitric oxide synthase
   inirradiated hepatoma cells.
   Br J Cancer. 2012 Feb 28;106(5):889-95. doi: 10.1038/bjc.2012.9. Epub 2012 Jan 24.
29.Xie Y, Zhang J, Ye S, He M, Ren R, Yuan D, Shao C.
   SirT1 regulates radiosensitivity of hepatoma cells differently under normoxic and
   hypoxicconditions.
   Cancer Sci. 2012 Jul;103(7):1238-44. doi: 10.1111/j.1349-7006.2012.02285.x.
   Epub 2012 Apr 27.
30.Xu L, Liu T, Han F, Zong Z, Wang G, Yu B, Zhang J
   AURKB and MAPK involvement in the regulation of the early stages of mouse
   zygotedevelopment.
   Sci China Life Sci. 2012 Jan;55(1):47-56.doi: 10.1007/s11427-012-4264-4.
   Epub 2012 Feb 8
31.Shen X, Wang C, Zhu Q, Li T, Yu L, Zheng W, Fei C, Qiu M, Xue F.
   Effect of the diclazuril on Hsp90 in the second-generation merozoites of Eimeria
   tenella.
   Vet Parasitol.2012 Apr 30;185(2-4):290-5. doi: 10.1016/j.vetpar.2011.10.018.
   Epub 2011 Oct 20.
32.Wang S, Wang J, Sun Y, Song Q, Li S.
   PKC-mediated USP phosphorylation at Ser35 modulates 20-hydroxyecdysone signaling in
   Drosophila.
   J Proteome Res. 2012 Dec 7;11(12):6187-96. doi: 10.1021/pr3008804. Epub 2012 Nov 16.
33.Shi QX, Zhang LJ, Yao Y, Zhang QY, Wang W, Li J, Shang YL, Bi H, Zhang SM, Guo HT,
   Wang YM, Yu SQ, Yi DH, Bueno FR, Kaye AD, Pei JM.
   κ-opioid Receptor Activation Prevents Against Arrhythmias by Preserving Cx43 Protein Via
   Alleviation of Intracellular Calcium.
   Am J Ther. 2012 Sep 24.
34.Liu P, Shi Y, Yang Y, Cao Y, Shi Y, Li H, Liu J, Lin J, Jin Y.
   Schistosoma japonicum UDP-glucose 4-epimerase protein is located on the tegument and
   induces moderateprotection against challenge infection.
   PLoS One. 2012;7(7):e42050. doi: 10.1371/journal.pone.0042050. Epub 2012 Jul 27.
35.Zhou J, Ge Z, Tan Y, Jiang G, Feng J, Wang H, Shi G.
   Downregulation of JWA expression in human esophageal squamous cell carcinoma and its
   clinical significance.
   Oncol Res. 2012;20(4):157-62.
36.Jiang L, Lin J, Han H, Dong H, Zhao Q, Zhu S, Huang B.
   Identification and characterization of Eimeria tenella apical membrane antigen-1 (AMA1).
   PLoS One. 2012;7(7):e41115. doi: 10.1371/journal.pone.0041115. Epub 2012 Jul 19.
37.Li J, Liang S, Jin H, Xu C, Ma D, Lu X.
   Tiam1, negatively regulated by miR-22, miR-183 and miR-31, is involved in migration,
   invasion and viability ofovarian cancer cells.
   Oncol Rep. 2012 Jun;27(6):1835-42. doi: 10.3892/or.2012.1744. Epub 2012 Mar 27.
38.Guo W, Zhang Z, Liu X, Burnstock G, Xiang Z, He C.
   Developmental expression of P2X5 receptors in the mouse prenatal central and peripheral
   nervous systems.
   Purinergic Signal. 2013 Jun;9(2):239-48. doi: 10.1007/s11302-012-9346-z.
   Epub 2012 Dec 28.
39.Duan J, Xu H, Ma S, Guo H, Wang F, Zhao P, Xia Q.
   Cre-mediated targeted gene activation in the middle silk glands of transgenic silkworms
   (Bombyx mori).
   Transgenic Res.2013 Jun;22(3):607-19.doi:10.1007/s11248-012-9677-0.Epub 2012 Dec 23.
40.Yu Y, Schachner M.
   Syntenin-a promotes spinal cord regeneration following injury in adult zebrafish.
   Eur J Neurosci. 2013 Apr 22. doi: 10.1111/ejn.12222.
41.Han HY, Zhu SH, Jiang LL, Li Y, Dong H, Zhao QP, Kong CL, Huang B.
   Molecular characterization and analysis of a novel calcium-dependent protein kinase from
   Eimeria tenella.
   Parasitology. 2013 May;140(6):746-55. doi: 10.1017/S0031182012002107. Epub 2013 Feb 1.
42.Lin Y, Meng Y, Wang YX, Luo J, Katsuma S, Yang CW, Banno Y, Kusakabe T, Shimada T,
   Xia QY.
   Vitellogenin receptor mutation leads to the oogenesis mutant phenotype "scanty vitellin"
   of the silkworm,Bombyx mori.
   J Biol Chem. 2013 May 10;288(19):13345-55. doi: 10.1074/jbc.M113.462556.
   Epub 2013 Mar 20.
43.Qian C, Wang Y, Chen Y, Zeng L, Zhang Q, Shuai X, Huang K.
   Suppression of pancreatic tumor growth by targeted arsenic delivery with anti-CD44v6
   single chain antibodyconjugated nanoparticles.
   Biomaterials. 2013 Aug;34(26):6175-84. doi: 10.1016/j.biomaterials.2013.04.056.
   Epub 2013 May 27.
44.Zhang Y, Yang L, Yang YJ, Liu XY, Jia JG, Qian JY, Wang KQ, Zuo J, Ge JB.
   Low-dose taurine upregulates taurine transporter expression in acute myocardial
   ischemia.
   Int J Mol Med. 2013 Apr;31(4):817-24. doi: 10.3892/ijmm.2013.1264. Epub 2013 Feb 1.
45.Zhao N, Yang K, Yang G, Chen D, Tang H, Zhao D, Zhao C.
   Aberrant expression of clock gene period1 and its correlations with the growth,
   proliferation and metastasis ofbuccal squamous cell carcinoma.
   PLoS One. 2013;8(2):e55894. doi: 10.1371/journal.pone.0055894. Epub 2013 Feb 6.
46.Ma WM, Li HW, Dai ZM, Yang JS, Yang F, Yang WJ.
   Chitin-binding proteins of Artemia diapause cysts participate in formation of the
   embryonic cuticle layer of cystshells.
   Biochem J. 2013 Jan 1;449(1):285-94. doi: 10.1042/BJ20121259.
47.Chen P, Tong XL, Li DD, Liang PF, Fu MY, Li CF, Hu H, Xiang ZH, Lu C, Dai FY.
   Fine mapping of a supernumerary proleg mutant (ECs -l) and comparative expression
   analysis of the abdominal-A gene in silkworm, Bombyx mori.
   Insect Mol Biol. 2013 Jun 27. doi: 10.1111/imb.12039.
48.Tian L, Ma L, Guo E, Deng X, Ma S, Xia Q, Cao Y, Li S.
   20-hydroxyecdysone upregulates Atg genes to induce autophagy in the Bombyx fat body.
   Autophagy. 2013 May 14;9(8).
49.Lin D, Cui Z, Kong L, Cheng F, Xu J, Lan F.
   p73 participates in WWOX-mediated apoptosis in leukemia cells.
   Int J Mol Med. 2013 Apr;31(4):849-54. doi: 10.3892/ijmm.2013.1289. Epub 2013 Feb 26.
50.Cui Z, Lin D, Cheng F, Luo L, Kong L, Xu J, Hu J, Lan F.
   The role of the WWOX gene in leukemia and its mechanisms of action.
   Oncol Rep. 2013 Jun;29(6):2154-62. doi: 10.3892/or.2013.2361. Epub 2013 Mar 22.
51.Li Z, Bi X, Wang M, Zhang J, Song J, Shen X, Han J, Fu G, Ye Y.
   Inhibition of farnesyl pyrophosphate synthase prevents angiotensin II-induced cardiac
   fibrosis in vitro.
   Clin Exp Immunol. 2014 Jun;176(3):429-37. doi: 10.1111/cei.12282.
52.Zhang J, He Q, Zhang CD, Chen XY, Chen XM, Dong ZQ, Li N, Kuang XX, Cao MY, Lu C,
   Pan MH.
   Inhibition of BmNPV replication in silkworm cells using inducible and regulated
   artificial microRNA precursors targeting the essential viral gene lef-11.
   Antiviral Res. 2014 Apr;104:143-52. doi: 10.1016/j.antiviral.2014.01.017.
   Epub 2014 Jan 31.
53.Ye Y, Miao S, Lu R, Xia X, Chen Y, Zhang J, Wu X, He S, Qiang F, Zhou J.
   Allograft inflammatory factor-1 is an independent prognostic indicator that regulates
   β-catenin in gastric cancer.
   Oncol Rep. 2014 Feb;31(2):828-34. doi: 10.3892/or.2013.2915. Epub 2013 Dec 12.
54.Tang C, Yang L, Jiang X, Xu C, Wang M, Wang Q, Zhou Z, Xiang Z, Cui H.
   Antibiotic drug tigecycline inhibited cell proliferation and induced autophagy in
   gastric cancer cells.
   Biochem Biophys Res Commun. 2014 Mar 28;446(1):105-12. doi: 10.1016/j.bbrc.
   2014.02.043. Epub 2014 Feb 28.
55.Tao X, Fan J, Kao G, Zhang X, Su L, Yin Y, Zrenner B.
   Angiotensin-(1-7) attenuates angiotensin II-induced signalling associated with
   activation of a tyrosine phosphatase in Sprague-Dawley rats cardiac fibroblasts.
   Biol Cell. 2014 Jun;106(6):182-92. doi: 10.1111/boc.201400015. Epub 2014 Apr 25.
56.Shang Y, Guo F, Li J, Fan R, Ma X, Wang Y, Feng N, Yin Y, Jia M, Zhang S, Zhou J,
   Wang H, Pei J.
   Activation of κ-opioid receptor exerts the glucose-homeostatic effect in
   streptozotocin-induced diabetic mice.
   J Cell Biochem. 2014 Sep 4. doi: 10.1002/jcb.24962.
57.Qian J, Wu C, Chen X, Li X, Ying G, Jin L, Ma Q, Li G, Shi Y, Zhang G, Zhou N.
   Differential requirements of arrestin-3 and clathrin for ligand-dependent and
   -independent internalization of human G protein-coupled receptor 40.
   Cell Signal. 2014 Nov;26(11):2412-23. doi: 10.1016/j.cellsig.2014.07.019.
   Epub 2014 Jul 16.