碧云天生物技术-ER-Tracker Red (内质网红色荧光探针)(C1041M)

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ER-Tracker Red (内质网红色荧光探针)

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产品编号: C1041M

产品包装:100μl

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产品编号	产品名称	产品包装	产品价格
C1041S	ER-Tracker Red (内质网红色荧光探针)	20μl	1303.00元
C1041M	ER-Tracker Red (内质网红色荧光探针)	100μl	3598.00元
C1041L	ER-Tracker Red (内质网红色荧光探针)	500μl	9998.00元

ER-Tracker Red是一种具有细胞膜通透性的内质网(endoplasmic reticulum, ER)红色荧光探针，对内质网有高度选择性，可以用于活细胞内质网特异性荧光染色。
ER-Tracker Red为采用Molecular Probes公司的BODIPY TR进行了荧光标记的glibenclamide。Glibenclamide即glyburide，中文名为格列苯脲，是一种2型糖尿病治疗药物，可以结合主要定位在内质网上的包含ATP敏感的钾离子通道(K_ATP channel)的磺脲类(sulphonylurea)受体。因此荧光标记的glibenclamide就可以用作内质网特异性的荧光探针。ER-Tracker Red适用于活细胞内质网的荧光染色，但不适合用于固定细胞内质网的荧光染色。
ER-Tracker Red的分子式为C₄₄H₄₂BClF₂N₆O₇S₂，分子量为915.23，ER-Tracker Red的化学结构式参考图1。
图1.ER-Tracker Red的化学结构式。 ER-Tracker Red对于细胞的毒性极低。而传统的DiOC6(3)对ER染色的同时也对细胞有一定的毒性。
ER-Tracker Red呈红色荧光，检测时的最大激发波长为587nm，最大发射波长为615nm。ER-Tracker Red的激发光谱和发射光谱参考图2。
图2.ER-Tracker Red的激发光谱和发射光谱。 ER-Tracker Red按照后附的使用说明染色后，用甲醛等固定后染色效果可以被部分保留。ER-Tracker Red在活细胞中的染色效果参考图2。
图3.ER-Tracker Red(内质网红色荧光探针)对于NRK-52E细胞(大鼠肾小管上皮细胞)的染色效果。Mito-Tracker Green染色的NRK-52E细胞其线粒体呈现绿色荧光(图B)，ER-Tracker Red染色的NRK-52E细胞其内质网呈现红色荧光(图C)，绿色荧光、红色荧光及细胞核蓝色荧光的叠加(merge)效果见图D。其中细胞核使用Hoechst 33342 (C1027)染色。本图仅作参考，实际检测效果会因实验条件、检测仪器等的不同而存在差异。
提供了ER-Tracker Red 稀释液，使ER-Tracker Red的使用更加便捷。
按照1:1000的比例稀释，每10µl可以配制10ml ER-Tracker Red工作液；按照1:3000的比例稀释，每10µl可以配制30ml ER-Tracker Red工作液。
包装清单：

产品编号	产品名称	包装
C1041S-1	ER-Tracker Red (1mM)	20µl
C1041S-2	ER-Tracker Red稀释液	60ml
—	说明书	1份

产品编号	产品名称	包装
C1041M-1	ER-Tracker Red (1mM)	100µl
C1041M-2	ER-Tracker Red稀释液	250ml
—	说明书	1份

产品编号	产品名称	包装
C1041L-1	ER-Tracker Red (1mM)	500µl
C1041L-2	ER-Tracker Red稀释液	500ml×2
—	说明书	1份

保存条件：
-20℃保存，半年有效。ER-Tracker Red需-20℃避光保存。
注意事项：
ER-Tracker Red(1mM)在4℃、冰浴等较低温度情况下会凝固而粘在离心管管底、管壁或管盖内，可以20-25℃水浴温育片刻至全部融解后使用。对于微量的液体，每次使用前先离心数秒钟，使液体充分沉降到管底。
荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。
需自备盖玻片和载玻片(可以向碧云天订购)。
格列本脲的药理学活性可能会影响内质网的一些功能；某些特殊细胞中磺脲类受体的可变表达可能会导致非内质网特异性染色。
ER-Tracker Red适用于活细胞内质网的荧光染色，但不适合用于固定细胞内质网的荧光染色。如果经ER-Tracker Red染色后的细胞需要进行固定操作，使用4%多聚甲醛在37℃固定2分钟。
ER-Tracker Red染色后的细胞不能用Triton X-100通透，Triton X-100通透处理会导致ER-Tracker Red的荧光染色消失。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1.ER-Tracker Red工作液的配制：
a.取少量ER-Tracker Red按照1:1000的比例加入到ER-Tracker Red 稀释液中。例如取1μl ER-Tracker Red加入到1ml ER-Tracker Red 稀释液中。混匀后即为ER-Tracker Red工作液。
b.ER-Tracker Red工作液使用前需37℃预温育。
注：工作液中ER-Tracker Red的浓度可以根据实际情况进行适当调整，推荐的稀释比例调整范围为1:1000-1:3000。为降低背景，在染色效果可以接受的范围内，建议尽量使用较低浓度的ER-Tracker Red。
2.内质网的荧光标记：
a.去除细胞培养液，用适量的溶液如HBSS with Ca²⁺ & Mg²⁺ (Hanks' Balanced Salt Solution with Ca²⁺ & Mg²⁺)洗涤生长在盖玻片上的细胞。注：HBSS with Ca²⁺ & Mg²⁺ (C0219)可以向碧云天订购；对于悬浮细胞的染色可以参考贴壁细胞的染色方法进行。
b.去除洗涤液，加入步骤1配制好的并37℃预温育的ER-Tracker Red染色工作液，与细胞37℃共孵育15-30分钟。
c.去除ER-Tracker Red染色工作液，用细胞培养液洗涤细胞1-2次。
d.随后通常用荧光显微镜或激光共聚焦显微镜进行观察。此时可观察到内质网呈明亮的强荧光染色。
e.如果经ER-Tracker Red染色后的细胞需要进行固定，可以使用4%甲醛37℃固定2分钟。固定后用适当的洗涤液洗涤2-3次，每次5分钟，随后可以进行复染或滴加适当的抗荧光淬灭封片液，最后封片观察。注意：ER-Tracker Red染色的细胞不能用Triton X-100通透，Triton X-100通透处理会导致ER-Tracker Red的荧光染色消失。

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C1991S	细胞膜红色荧光染色试剂盒(DiI)	200-1000次
C1993S	细胞膜绿色荧光染色试剂盒(DiO)	200-1000次
C1995S	细胞膜远红外荧光染色试剂盒(DiD)	200-1000次
C2005	JC-1	1mg
C2007	Rhodamine 123	5mg

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47. Li X, Du X, Ni J

Zn2+ Aggravates Tau Aggregation and Neurotoxicity.

Int J Mol Sci. 2019 Jan 23 20(3). pii: E487. (IF 4.556)

48. Xu D, Wu L, Jiang X, Yang L, Cheng J, Chen H, Hua R, Geng G, Yang L, Li Q

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49. Jiang YJ, Lin M, Yang T, Li RS, Huang CZ, Wang J, Li YF

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J Mater Chem B. 2019 Mar 28 7(12):2074-2080. (IF 5.344)

50. Chen P, Zhao M, Chen Q, Fan L, Gao F, Zhao L

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51. Liang XD, Zhang YN, Liu CC, Chen J, Chen XN, Sattar Baloch A, Zhou B

U18666A inhibits classical swine fever virus replication through interference with intracellular cholesterol trafficking.

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52. Dong C, Tu W, He M, Fu J, Kobayashi A, Konishi T, Shao C

Role of Endoplasmic Reticulum and Mitochondrion in Proton Microbeam Radiation-Induced Bystander Effect.

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53. Xia Q, Wang X, Liu Y, Shen Z, Ge Z, Huang H, Li X, Wang Y

An endoplasmic reticulum-targeted two-photon fluorescent probe for bioimaging of HClO generated during sleep deprivation.

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54. Zhaoyang Wang, Jiaoyan Jia, Lu Wang, Feng Li, Yiliang Wang, Yuzhou Jiang, Xiaowei Song, Shurong Qin, Kai Zheng, Ju Ye, Zhe Ren, Yifei Wang, Shuhua Qi

Anti-HSV-1 activity of Aspergillipeptide D, a cyclic pentapepetide isolated from fungus Aspergillus sp. SCSIO 41501

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55. Ru-Jia Xie, Xiao-Xia Hu, Lu Zheng, Shuang Cai, Yu-Si Chen, Yi Yang, Ting Yang, Bing Han, Qin Yang

Calpain-2 activity promotes aberrant endoplasmic reticulum stress-related apoptosis in hepatocytes

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56. Ming-Hong Sun, Xiao-Han Li, Yao Xu, Yi Xu, Zhen-Nan Pan, Shao-Chen Sun

Citrinin exposure disrupts organelle distribution and functions in mouse oocytes

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57. Zhi-Fei Liu, Jian-Fei Ji, Xiao-Feng Jiang, Tong Shao, Dong-Dong Fan, Xin-Hang Jiang, Ai-Fu Lin, Li-Xin Xiang, Jian-Zhong Shao

Characterization of cGAS homologs in innate and adaptive mucosal immunities in zebrafish gives evolutionary insights into cGAS-STING pathway

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58. Zhen-Nan Pan, Meng-Hao Pan, Ming-Hong Sun, Xiao-Han Li, Yu Zhang, Shao-Chen Sun

RAB7 GTPase regulates actin dynamics for DRP1-mediated mitochondria function and spindle migration in mouse oocyte meiosis

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59. Fan Xiao, Meixin Gao, Junru Yang, Lingling He, Hongshan Wei

FAM172A Deletion May Enhance Hepatic Steatosis by Promoting ER Stress

DIGEST DIS SCI. 2020 Sep 18. doi: 10.1007/s10620-020-06601-y. (IF 2.751)

60. Xiuge Gao, Xiangchun Ruan, Hui Ji, Lin Peng, Yawei Qiu, Dan Yang, Xinhao Song, Chunlei Ji, Dawei Guo, Shanxiang Jiang

Maduramicin triggers methuosis-like cell death in primary chicken myocardial cells

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61. Yongnan Li, Shuodong Wu

Curcumin inhibits the proteolytic process of SREBP-2 by first inhibiting the expression of S1P rather than directly inhibiting SREBP-2 expression

Food Sci Nutr. 2020 Nov 8;9(1):209-216. doi: 10.1002/fsn3.1985. (IF 1.797)

62. Yajun Lu, Yuxiao Yang, Siqi Yang, Qianfeng Xia

Immunomodulatory action of excretory-secretory products of Angiostrongylus cantonensis in a mouse tumour model

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63. Qi Yang, Rongjuan Pei, Yun Wang, Yuan Zhou, Min Yang, Xinwen Chen, Jizheng Chen

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J Virol. 2021 Jan 28;95(4):e01926-20. doi: 10.1128/JVI.01926-20. (IF 4.501)

64. Sijing Hu, Hao Zhang, Yunqiang Liu, Mohan Liu, Jingjing Li, Shunyao Liao

Whole-exome sequencing of de novo genetic variants in a Chinese family with a sporadic case of congenital nonsyndromic hearing loss

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65. Yan Hui Yang, Heng Yang, Rui Fang Li, Cui Xiang Li, Lei Zeng, Chao Jie Wang, Na Li, Zhuang Luo

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67. Kangyong Chen, Jiayin Tian, Junya Wang, Zhao Jia, Qin Zhang, Wenji Huang, Xin Zhao, Zhipeng Gao, Qian Gao, Jun Zou

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69. Ya-Yun Liu, Xiao-Dong Liang, Chun-Chun Liu, Yan Cheng, Huan Chen, Abdul Sattar Baloch, Jin Zhang, Yun Young Go, Bin Zhou

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71. Rongjin Luo, Shuai Li, Gaocai Li, Saideng Lu, Weifeng Zhang, Hui Liu, Jie Lei, Liang Ma, Wencan Ke, Zhiwei Liao, Bingjin Wang, Yu Song, Kun Wang, Yukun Zhang, Cao Yang

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72. Jinglun Zhang, Qian Wu, Chengcheng Yin, Xiaoshi Jia, Zifan Zhao, Xiaoxin Zhang, Guohua Yuan, Hao Hu, Qin Zhao

Sustained calcium ion release from bioceramics promotes CaSR-mediated M2 macrophage polarization for osteoinduction

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73. Lin Meng, Hongmei Hu, Zhiqiang Liu, Luyao Zhang, Qingrui Zhuan, Xue Li, Xiangwei Fu, Shien Zhu, Yunpeng Hou

The Role of Ca2 + in Maturation and Reprogramming of Bovine Oocytes: A System Study of Low-Calcium Model

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74. Zhihao Liu, Xiyun Bian, Wenbo Gao, Jing Su, Chuanrui Ma, Xiaolin Xiao, Tian Yu, Han Zhang, Xiaozhi Liu, Guanwei Fan

Rg3 promotes the SUMOylation of SERCA2a and corrects cardiac dysfunction in heart failure

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75. Yi-Ming Li, Shao-Yang Zhao, Huan-Huan Zhao, Bao-Hua Wang, Sai-Mei Li

Procyanidin B2 Alleviates Palmitic Acid-Induced Injury in HepG2 Cells via Endoplasmic Reticulum Stress Pathway

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76. Lin Lei, Wenwen Gao, Juan J Loor, Ahmad Aboragah, Zhiyuan Fang, Xiliang Du, Min Zhang, Yuxiang Song, Guowen Liu, Xinwei Li

Reducing hepatic endoplasmic reticulum stress ameliorates the impairment in insulin signaling induced by high levels of β-hydroxybutyrate in bovine hepatocytes

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77. Shaojuan Song, Xin Xia, Jiajia Qi, Xiaopei Hu, Qian Chen, Jiang Liu, Ning Ji, Hang Zhao

Silmitasertib-induced macropinocytosis promoting DDP intracellular uptake to enhance cell apoptosis in oral squamous cell carcinoma

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78. Hao Zhao, Yue Xu, Xinlei Song, Qingchen Zhang, Yajie Wang, Haiyan Yin, Xiaohui Bai, Jianfeng Li

Cisplatin induces damage of auditory cells: Possible relation with dynamic variation in calcium homeostasis and responding channels

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79. Jianzhao Liao, Zhuoying Hu, Quanwei Li, Hongji Li, Weijin Chen, Haihua Huo, Qingyue Han, Hui Zhang, Jianying Guo, Lianmei Hu, Jiaqiang Pan, Ying Li, Zhaoxin Tang

Endoplasmic Reticulum Stress Contributes to Copper-Induced Pyroptosis via Regulating the IRE1α-XBP1 Pathway in Pig Jejunal Epithelial Cells

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80. Meng-Meng Shan, Yuan-Jing Zou, Zhen-Nan Pan, Hao-Lin Zhang, Yi Xu, Jia-Qian Ju, Shao-Chen Sun

Kinesin motor KIFC1 is required for tubulin acetylation and actin-dependent spindle migration in mouse oocyte meiosis

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81. Lin Zhu, Bin Li, Dongqin Chen, Ning Chen, Le Xu, Qinjin Li, Xiaodong Chen

sSTEAP4 regulates cellular homeostasis and improves high-fat-diet-caused oxidative stress in hepatocytes

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82. Fangjiao Lv, Yang Xu, Dean W Gabriel, Xue Wang, Ning Zhang, Wenxing Liang

Quantitative Proteomic Analysis Reveals Important Roles of the Acetylation of ER-Resident Molecular Chaperones for Conidiation in Fusarium oxysporum

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83. Yu-Shen Luo, Quan-Kuo He, Ming-Xin Sun, Feng-Xin Qiao, Yue-Cen Liu, Chang-Long Xu, Zhi-Ran Xu, Si-Cheng Zhao, Hai-Long Wang, Zhong-Quan Qi, Yu Liu

Acrylonitrile exposure triggers ovarian inflammation and decreases oocyte quality probably via mitochondrial dysfunction induced apoptosis in mice

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84. Shuanghang Liu, Tingxian Deng, Liping Hua, Xinzhe Zhao, Hanxiao Wu, Peihao Sun, Mingxiao Liu, Shujun Zhang, Liguo Yang, Aixin Liang

Novel functional mutation of the PDIA3 gene affects milk composition traits in Chinese Holstein cattle

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85. Yunyi Liu, Juan Li, Hailong Ou, Dan Qi, Bei Hu, Yuxi Xu, Jian Hu, Yi Xiong, Luling Xia, Jason H Huang, Xiaoxiao Hu, Erxi Wu

Identification of new aptamer BC-3 targeting RPS7 from rapid screening for bladder carcinoma

Genes Dis. 2022 Aug 3;10(5):2137-2150. doi: 10.1016/j.gendis.2022.07.002.

86. Huidi Xie, Yang Shi, Ying Zhou, Hongfang Liu

TMBIM6 promotes diabetic tubular epithelial cell survival and albumin endocytosis by inhibiting the endoplasmic reticulum stress sensor, IRE1α

Mol Biol Rep. 2022 Oct;49(10):9181-9194. doi: 10.1007/s11033-022-07744-z. (IF 1.402)

87. Yue Li, Ran Yao, Miao Ren, Ke Yuan, Yuwei Du, Yuan He, Haiquan Kang, Shengnan Yuan, Wen Ju, Jianlin Qiao, Kailin Xu, Lingyu Zeng

Liposomes trigger bone marrow niche macrophage "foam" cell formation and affect hematopoiesis in mice

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88. Yan Li, Hong-Ying Li, Jun Shao, Lingpeng Zhu, Tian-Hua Xie, Jiping Cai, Wenjuan Wang, Meng-Xia Cai, Zi-Li Wang, Yong Yao, Ting-Ting Wei

GRP75 Modulates Endoplasmic Reticulum-Mitochondria Coupling and Accelerates Ca2+-Dependent Endothelial Cell Apoptosis in Diabetic Retinopathy

Biomolecules. 2022 Nov 29;12(12):1778. doi: 10.3390/biom12121778. (IF 4.082)

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Site-directed late-stage diversification of macrocyclic nannocystins facilitating anticancer SAR and mode of action studies

RSC Med Chem. 2022 Dec 22;14(2):299-312. doi: 10.1039/d2md00393g.

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(E)-2-styrylanthracene-9,10-dione derivatives as novel fluorescent probes: synthesis, photophysical properties and application in mitochondria imaging

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Feb 5:286:121988. doi: 10.1016/j.saa.2022.121988.

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