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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| P2451-10μg | Recombinant Human Cdc42 (Flag-Tag) | 10μg | 368.00元 |
| P2451-100μg | Recombinant Human Cdc42 (Flag-Tag) | 100μg | 1298.00元 |
| P2451-1mg | Recombinant Human Cdc42 (Flag-Tag) | 1mg | 8698.00元 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Human | 998 | P60953 | E.coli | 198aa | ~22kDa | C-Flag Tag |
| 蛋白信息(About this protein) | |
| 名称(Name) | Recombinant Human Cdc42，重组人Cdc42蛋白 |
| 别名(Synonyms) | Cell division control protein 42, Cell division cycle 42, G25K GTP-binding protein, CDC42Hs, G25K, TKS |
| 产品简介 (Background) |
Beyotime's recombinant human Cdc42 (rhCdc42) was expressed in E.coli and purified, which contains the mature form Cdc42 (2-188aa) with Flag tag (DYKDDDDK) at the C-terminus. Cdc42 is involved in the regulation of a variety of tumor and non-tumor diseases through a cascade of multiple signaling pathways. Active Cdc42 can regulate intercellular adhesion, cytoskeleton formation, and cell cycle, thus affecting cell proliferation, transformation, and dynamic balance as well as migration and invasion of tumor cells by regulating the expression of effector proteins [1]. |
| 产品用途 (Applications) |
Recombinant Human Cdc42 is useful in studying Cdc42 interacting proteins, effectors, GAPs (GTPase-activating proteins) and GEFs (Guanine nucleotide-exchange factors). It can also be used as positive control in Western blot. |
| 外观 (Physical Appearance) |
Liquid |
| 活性(Biological activity) | About 0.57nmol/h/μg in GTPase activity assay. |
| 浓度(Concentration) | 1mg/ml |
| 纯度(Purity) | ≥95% by SDS-PAGE |
| 储存液(Storage buffer) | 50mM Tris pH 7.4, 0.5mM MgCl2, 50mM NaCl, 1mM DTT |
图1. 小GTP酶在“激活”和“静息”状态之间循环的原理图。
小GTP酶(Small GTPase)，也称Small G-proteins、Ras superfamily，是调节真核细胞信号转导、细胞增殖、细胞骨架重组和细胞内膜转运等过程的分子开关，小GTP酶通过结合和水解GTP，在“激活”和“静息”状态之间循环：在外界信号的刺激下，鸟苷酸交换因子(Guanine nucleotide-exchange factors, GEFs)辅助小GTP酶将结合的GDP置换为GTP，小GTP酶结合GTP进入激活状态(Active state)；激活状态的小GTP酶与下游效应蛋白(Effector protein)相互作用，从而刺激细胞发生相应的响应；GTP酶激活蛋白(GTPase-activating proteins, GAPs)催化小GTP酶结合的GTP水解为GDP，并释放自由磷酸盐(Free phosphate, Pi)，此时小GTP酶结合GDP进入静息状态(Inactive state)，鸟嘌呤核苷酸解离抑制蛋白(Guanine nucleotide dissociation inhibitors, GDIs)抑制小GTP酶释放GDP，直到GEFs受到刺激信号再次开启新一轮的循环(图1) [2-3]。
小GTP酶在人类中已发现超过150个家族成员，在果蝇(Drosophila)、秀丽隐杆线虫(C. elegans)、酿酒酵母(S. cerevisiae)、粟酒裂殖酵母(S. pombe)、黏菌(Dictyostelium)和植物中也都发现了保守的同源物。小GTP酶根据结构和功能被分为5个家族分支：Ras家族、Rho家族、Ran家族、Rab家族和Arf家族。Ras家族本身又被分为6个亚家族：Ras亚家族、Ral亚家族、Rit亚家族、Rap亚家族、Rheb亚家族和Rad亚家族[4]。
Rho家族(Ras homologous GTPases)在真核生物中高度保守，是调节肌动蛋白重组的关键因子，因此在细胞迁移、伤口愈合、细胞粘附、细胞极性、膜转运和细胞分裂等过程中发挥着重要作用。目前有21个成员被发现，被分为8亚家族(见下表)，其中RhoA、Rac1和Cdc42研究的最为广泛[5]。
| Subfamily | Rho | Rac | Cdc42 | RhoH | RhoBTB | RhoUV | Rnd | RhoF |
| Rho family members | RhoA RhoB RhoC |
Rac1 Rac2 Rac3 RhoG |
Cdc42 RhoQ (TC10) RhoJ (TCL) |
RhoH | RhoBTB1 RhoBTB2 RhoBTB3 |
RhoU (Wrch) RhoV (Chp) |
Rnd1 Rnd2 Rnd3 (RhoE) |
RhoD RhoF (Rif) |
本产品经SDS-PAGE电泳检测蛋白纯度和分子量参考图2。
图2. 碧云天Recombinant Human Cdc42 (Flag-Tag) (P2451)的SDS-PAGE电泳检测图。本蛋白经BeyoGel™ Elite PAGE预制胶(Bis-Tris, 12%, 10孔) (P0849)电泳，Marker为BeyoColor™彩色预染蛋白分子量标准(6.5-270kD) (P0071/P0072)，经BeyoBlue™考马斯亮蓝超快染色液(P0017F)染色。实际检测结果可能会因样品和检测条件等的不同而存在差异，图中数据仅供参考。
本产品经GTPase Activity Assay Kit (Colorimetric) (P2435)检测GTP酶活性的效果请参考图3。
图3. 碧云天Recombinant Human Cdc42 (Flag-Tag) (P2451)的GTP酶活性检测效果图。X轴为Cdc42蛋白的质量(μg)，Y轴为对应单位时间内释放Pi摩尔量(nmol/h)，建立二元一次方程的斜率即为GTP酶活性(nmol/h/μg)。实际结果因实验条件、检测仪器等的不同而存在差异，图中数据仅供参考。
包装清单：| 产品编号 | 产品名称 | 包装 |
| P2451-10μg | Recombinant Human Cdc42 (Flag-Tag) (1mg/ml) | 10μl |
| P2451-100μg | Recombinant Human Cdc42 (Flag-Tag) (1mg/ml) | 100μl |
| P2451-1mg | Recombinant Human Cdc42 (Flag-Tag) (1mg/ml) | 1ml |
| — | 说明书 | 1份 |
-20ºC保存，一年有效。-80ºC保存，可以保存更长时间。
注意事项：由于蛋白每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后，须分装后-20ºC或更低温度冻存，尽量避免反复冻融。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| 产品编号 | 产品名称 | 包装 |
| P2061 | Rhotekin-RBD Agarose (活性Rho结合琼脂糖凝胶) | 1ml |
| P2431 | Raf1-RBD Agarose (活性Ras结合琼脂糖凝胶) | 1ml |
| P2437 | PAK1-PBD Agarose (活性Cdc42/Rac结合琼脂糖凝胶) | 1ml |
| P2065 | Active Rho Pull-down and Detection Kit | 50次/250次 |
| P2433 | Active Ras Pull-down and Detection Kit | 50次/250次 |
| P2438 | Active Cdc42 Pull-down and Detection Kit | 50次/250次 |
| P2439 | Active Rac1 Pull-down and Detection Kit | 50次/250次 |
| P2435 | GTP酶活性检测试剂盒(显色法) | 500次/2500次 |
| P2063 | Recombinant Human RhoA (Flag-Tag) | 10μg/100μg/1mg |
| P2401 | Recombinant Human NRas (Flag-Tag) | 10μg/100μg/1mg |
| P2403 | Recombinant Human HRas (His-Tag) | 10μg/100μg/1mg |
| P2405 | Recombinant Human KRas4A (His-Tag) | 10μg/100μg/1mg |
| P2407 | Recombinant Human KRas4B (His-Tag) | 10μg/100μg/1mg |
| P2409 | Recombinant Human KRas4B (G12C, His-Tag) | 10μg/100μg/1mg |
| P2411 | Recombinant Human KRas4B (G12D, His-Tag) | 10μg/100μg/1mg |
| P2413 | Recombinant Human KRas4B (G12V, His-Tag) | 10μg/100μg/1mg |
| P2415 | Recombinant Human KRas4B (G13D, His-Tag) | 10μg/100μg/1mg |
| P2417 | Recombinant Human KRas4B (G13S, His-Tag) | 10μg/100μg/1mg |
| P2419 | Recombinant Human KRas4B (Q61H, His-Tag) | 10μg/100μg/1mg |
| P2451 | Recombinant Human Cdc42 (Flag-Tag) | 10μg/100μg/1mg |
| P2453 | Recombinant Human Cdc42 (Q61L, Flag-Tag) | 10μg/100μg/1mg |
| P2455 | Recombinant Human Cdc42 (G12V, Flag-Tag) | 10μg/100μg/1mg |
| P2461 | Recombinant Human Rac1 (Flag-Tag) | 10μg/100μg/1mg |
| P2463 | Recombinant Human Rac1 (Q61L, Flag-Tag) | 10μg/100μg/1mg |
| P2465 | Recombinant Human Rac1 (T17N, Flag-Tag) | 10μg/100μg/1mg |
| P2469 | Recombinant Human Rac2 (Flag-Tag) | 10μg/100μg/1mg |
| AF0273 | Ras Rabbit Polyclonal Antibody | 50μl |
| AF1168 | Ras Rabbit Monoclonal Antibody | 50μl |
| AF7131 | HRas Rabbit Polyclonal Antibody | 50μl |
| AF7347 | KRas Rabbit Polyclonal Antibody | 50μl |
| AG2391 | KRas Rabbit Monoclonal Antibody | 50μl |
| AG2394 | KRas Mouse Monoclonal Antibody | 50μl |
| AF2794 | CDC42 Rabbit Monoclonal Antibody | 50μl |
| AG3059 | Rac1 Mouse Monoclonal Antibody | 50μl |
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