使用说明：
1. 完全溶解蛋白标准品，取10μl稀释至100μl，使终浓度为0.5mg/ml。蛋白样品在什么溶液中，标准品也
   宜用什么溶液稀释。但是为了简便起见，也可以用0.9%NaCl或PBS稀释标准品。
2. 将标准品按0, 1, 2, 4, 8, 12, 16, 20μl加到96孔板的标准品孔中，加标准品稀释液补足到20μl。
3. 加适当体积样品到96孔板的样品孔中，加标准品稀释液到20μl。
4. 各孔加入200μl G250染色液，室温放置3-5分钟。
5. 用酶标仪测定A595，或560-610nm之间的其它波长的吸光度。
6. 根据标准曲线计算出样品中的蛋白浓度。

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82.Zhu S, Wang Y, Chen M, Jin J, Qiu Y, Huang M, Huang Z.
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83.Tan Y, Xing J, Zhan W.
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85.Wu S, Ju GQ, Du T, Zhu YJ, Liu GH.
   Microvesicles derived from human umbilical cord Wharton's jelly mesenchymal stem cells
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90.Ma W, Kang F, Wang Z, Yang W, Li G, Ma X, Li G, Chen K, Zhang Y, Wang J.
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96.Li H, Zhang L, Cui Y, Luo X, Xue C, Wang S.
   Expression of soluble recombinant transglutaminase from Zea mays in Pichia pastoris.
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112.Yang Y, Qiu Y, Wang W, Xiao W, Liang H, Zhang C, Yang H, Teitelbaum DH, Sun LH, Yang H.
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