使用说明：
1.蛋白标准品的准备
a.取0.8ml蛋白标准配制液加入到一管蛋白标准(20mg BSA)中，充分溶解后配制成25mg/ml的蛋白标准溶液。配制后可立即使用，也可以-20℃长期保存。
b.取适量25mg/ml蛋白标准，稀释至终浓度为0.5mg/ml。例如取20μl 25mg/ml蛋白标准，加入980μl稀释液即可配制成0.5mg/ml蛋白标准。蛋白样品在什么溶液中，标准品也宜用什么溶液稀释。但是为了简便起见，也可以用0.9% NaCl或PBS稀释标准品。稀释后的0.5mg/ml蛋白标准可以-20℃长期保存。
2.BCA工作液配制
根据样品数量，按50体积BCA试剂A加1体积BCA试剂B(50:1)配制适量BCA工作液，充分混匀。例如5mlBCA试剂A加100μl BCA试剂B，混匀，配制成5.1ml BCA工作液。BCA工作液室温24小时内稳定。
3.蛋白浓度测定
a.将标准品按0、1、2、4、8、12、16、20μl加到96孔板的标准品孔中，加标准品稀释液补足到20μl，相当于标准品浓度分别为0、0.025、0.05、0.1、0.2、0.3、0.4、0.5mg/ml。
b.加适当体积样品到96孔板的样品孔中。如果样品不足20μl，需加标准品稀释液补足到20μl。请注意记录样品体积。
c.各孔加入200μl BCA工作液，37℃放置20-30分钟。
注: 也可以室温放置2小时，或60℃放置30分钟。BCA法测定蛋白浓度时，颜色会随着时间的延长不断加深。并且显色反应会因温度升高而加快。如果浓度较低，适合在较高温度孵育，或适当延长孵育时间。
d.用酶标仪测定A562，或540-595nm之间的其他波长的吸光度。
e.根据标准曲线和使用的样品体积计算出样品的蛋白浓度。
常见问题：
1.测定标准曲线时发现随着标准品浓度的增加吸光度或颜色没有明显变化。
可能的原因是样品中含有严重干扰BCA法测定蛋白浓度的物质，详细的BCA法的兼容性列表请参考碧云天如下网页:http://www.beyotime.com/Compatibility Chart For BCA Kit.pdf
2.是否每次测定时都需要做标准曲线？
建议每次测定时都做标准曲线。因为BCA法测定时颜色会随着时间的延长不断加深，并且显色反应的速度和温度有关，所以除非精确控制显色反应的时间和温度，否则如需精确测定宜每次都做标准曲线。

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   FEBS Lett. 2009 Sep 17;583(18):2997-3003.
87.Shi GS, Ren LF, Wang LZ, Lin HS, Wang SB, Tong YQ.
   H2O2/HCl and heat-treated Ti-6Al-4V stimulates pre-osteoblast proliferation and
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88.Zhu J, Zhang L, Jin X, Han X, Sun C, Yan J.
   beta-Ionone-induced apoptosis in human osteosarcoma (U2os) cells occurs via a
   p53-dependent signaling pathway.
   Mol Biol Rep. 2009 Sep 16.
89.Chen Y, Wang J, Yao Y, Yuan W, Kong M, Lin Y, Geng D, Nie R.
   CRP regulates the expression and activity of tissue factor as well as tissue factor
   pathway inhibitor via NF-kappaB and ERK 1/2 MAPK pathway.
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90.Chen WL, Qian Y, Meng WF, Pang JY, Lin YC, Guan YY, Chen SP, Liu J, Pei Z, Wang GL.
   A novel marine compound xyloketal B protects against oxidized LDL-induced cell injury in
   vitro.
   Biochem Pharmacol. 2009 Oct 15;78(8):941-50.
91.Sun H, Li QW, Lv XY, Ai JZ, Yang QT, Duan JJ, Bian GH, Xiao Y, Wang YD, Zhang Z, Liu
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   MicroRNA-17 post-transcriptionally regulates polycystic kidney disease-2 gene and
   promotes cell proliferation.
   Mol Biol Rep. 2009 Oct 10.
92.Wang S, Lv X, Zhang K, Lin T, Liu X, Yuan J, Dai Y, Li N.
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93.Ma K, Hu MX, Qi Y, Zou JH, Qiu LY, Jin Y, Ying XY, Sun HY.
   PAMAM-triamcinolone acetonide conjugate as a nucleus-targeting gene carrier for enhanced
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94.Hua W, Jiang J, Rong X, Wu R, Qiu H, Zhang Y, Chen Q.
   The dual role of the cystathionine gamma-lyase/hydrogen sulfide pathway in CVB3-induced
   myocarditis in mice.
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95.Jin D, Ojcius DM, Sun D, Dong H, Luo Y, Mao Y, Yan J.
   Leptospira interrogans induces apoptosis in macrophages via caspase-8- and caspase-3-
   dependent pathways.
   Infect Immun. 2009 Feb;77(2):799-809. Epub 2008 Nov 24.
96.Lin Y, Chen Y, Yang X, Xu D, Liang S.
   Proteome analysis of a single zebrafish embryo using three different digestion
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97.Xu Y, Ge R, Du J, Xin H, Yi T, Sheng J, Wang Y, Ling C.
   Corosolic acid induces apoptosis through mitochondrial pathway and caspase activation in
   human cervix adenocarcinoma HeLa cells.
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98.Li H, Zhang L, Huang Q.
   Differential expression of mitogen-activated protein kinase signaling pathway in the
   hippocampus of rats exposed to chronic unpredictable stress.
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99.Yi Q, Wen X, Li L, He B, Nie Y, Yao Wu Y, Zhang Z, Gu Z.
   The chiral effects on the responses of osteoblastic cells to the polymeric substrates.
   European Polymer Journal 45 (2009) 1970–1978.
100.ZHANG ZY, LIU XJ, LIU T, YAN L, WANG YJ, WANG CB.
   Polypeptide from Chlamys farreri inhibits UVB-induced apoptosis of HaCaT cells via
   iNOS/NO and HSP90.
   Chinese Journal of Oceanology and Limnology. Vol. 27 No. 3, P. 594-599, 2009.
101.Deng X, Cheng J, Zhang Y, Li N, Chen L.
   Effects of caloric restriction on SIRT1 expression and apoptosis of islet beta cells in
   type 2 diabetic rats.
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102.WU T, NAN KH, CHEN JD, JIN D, JIANG S, ZHAO PR, XU JC, DU H, ZHANG XQ, LI JW, PEI GX.
   A new bone repair scaffold combined with chitosan/hydroxyapatite and sustained releasing
   icariin.
   Chinese Science Bulletin. 2009; vol. 54 no. 17.
103.Fan L, Lin C, Zhuo S, Chen L, Liu N, Luo Y, Fang J, Huang Z, Lin Y, Chen J.
   Transplantation with survivin-engineered mesenchymal stem cells results in better
   prognosis in a rat model of myocardial infarction.
   Eur J Heart Fail. 2009;11(11):1023-30.
104.Liu XJ, Wang WC, Wang HJ, Zhang LL, Liu LQ, Wang YJ, Wang CB.
   A polypeptide from Chlamys farreri inhibits UVB-induced HaCaT cells apoptosis via the
   Apaf-1/caspase-9 and Smac/XIAP signaling pathway.
   Chinese Journal of Oceanology and Limnology.Vol. 27 No. 3, P. 587-593, 2009
105.Gong HS, Meng XC, Wang H.
   Plantaricin MG active against Gram-negative bacteria produced by Lactobacillus plantarum
   KLDS1.0391 isolated from ‘‘Jiaoke”, a traditional fermented cream from China.
   Food Control 21 (2010) 89–96.
106.Zhou CL, Yu XJ, Chen LM, Jiang H, Li CY.
   Corticotropin-releasing hormone attenuates vascular endothelial growth factor release
   from human HaCaT keratinocytes.
   Regul Pept. 2010 Feb 25;160(1-3):115-20.
107.Chen S, Chen Y, Hu C, Jing H, Cao Y, Liu X.
   Association of clinicopathological features with UbcH10 expression in colorectal cancer.
   J Cancer Res Clin Oncol. 2010 Mar;136(3):419-26.
108.Yuan D, Pan Y, Zhang J, Shao C.
   Role of nuclear factor-kappaB and P53 in radioadaptive response in Chang live cells.
   Mutat Res. 2010 Jun 1;688(1-2):66-71. Epub 2010 Mar 20.
109.Li F, Gong QH, Wu Q, Lu YF, Shi JS.
   Icariin isolated from Epimedium brevicornum Maxim attenuates learning and memory
   deficits induced by d-galactose in rats.
   Pharmacol Biochem Behav. 2010;96(3):301-5. Epub 2010 Jun 1.
110.Li H, Fong C, Chen Y, Cai G, Yang M.
   beta2- and beta3-, but not beta1-adrenergic receptors are involved in osteogenesis of
   mouse mesenchymal stem cells via cAMP/PKA signaling.
   Arch Biochem Biophys. 2010 15;496(2):77-83. Epub 2010 Feb 4.
111.Wu GJ, Zhou LZ, Wang KW, Chen F, Sun Y, Duan YR, Zhu YJ, Gu HC.
   Hydroxylapatite nanorods: An efficient and promising carrier for gene transfection.
   J Colloid Interface Sci. 2010.
112.Han J, Zhou YP, Jiang YZ, He YT, Mei F.
   Postnatal development of interstitial cells of Cajal in mouse colon in response to Kit
   signal blockade with Imatinib (Glivec).
   Acta Histochem. 2010;112(3):215-21. Epub 2010 Mar 2.
113.Feng B, Guo YW, Huang CG, Li L, Chen RH, Jiao BH.
   2'-epi-2'-O-Acetylthevetin B extracted from seeds of Cerbera manghas L. induces cell
   cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells.
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114.Qin XL, Bi HC, Wang XD, Li JL, Wang Y, Xue XP, Chen X, Wang CX, Xu le J, Wang YT,
   Huang M.
   Mechanistic understanding of the different effects of Wuzhi Tablet (Schisandra
   sphenanthera extract) on the absorption and first-pass intestinal and hepatic metabolism
   of Tacrolimus (FK506).
   Int J Pharm. 2010;389(1-2):114-21. Epub 2010 Jan 25.
115.Lin Y, Xu J, Cao L, Han Y, Gao J, Xie N, Zhao X, Jiang H, Chi Z.
   Mitochondrial base excision repair pathway failed to respond to status epilepticus
   induced by pilocarpine.
   Neurosci Lett. 2010;474(1):22-5. Epub 2010 Mar 3.
116.Li Q, Wu D, Zhang L, Zhang Y.
   Effects of galantamine on β-amyloid release and beta-site cleaving enzyme 1 expression
   in differentiated human neuroblastoma SH-SY5Y cells.
   Exp Gerontol. 2010;45(11):842-7. Epub 2010 Jul 3.
117.Li GG, Liang XF, Xie Q, Li G, Yu Y, Lai K.
   Gene structure, recombinant expression and functional characterization of grass carp
   leptin.
   Gen Comp Endocrinol. 2010;166(1):117-27. Epub 2009 Oct 24.
118.Zhao L, Wang X, Chang Q, Xu J, Huang Y, Guo Q, Zhang S, Wang W, Chen X, Wang J.
   Neferine, a bisbenzylisoquinline alkaloid attenuates bleomycin-induced pulmonary
   fibrosis.
   Eur J Pharmacol. 2010;627(1-3):304-12. Epub 2009 Nov 10.
119.Zhao L, Yang R, Cheng L, Wang M, Jiang Y, Wang S.
   LPS-Induced Epithelial-Mesenchymal Transition of Intrahepatic Biliary Epithelial Cells.
   J Surg Res. 2010.
120.He F, Zhang F, Yang G, Wang X, Zhao S.
   Enhanced initial proliferation and differentiation of MC3T3-E1 cells on HF/HNO3 solution
   treated nanostructural titanium surface.
   Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010;110(4):e13-22. Epub 2010
   Jul 15.
121.Yan J, Sun XB, Wang HQ, Zhao H, Zhao XY, Xu YX, Guo JC, Zhu CQ.
   Chronic restraint stress alters the expression and distribution of phosphorylated tau
   and MAP2 in cortex and hippocampus of rat brain.
   Brain Res. 2010;1347:132-41. Epub 2010 Jun 1.
122.Song XZ, Xu JQ, Wang T, Liu FH.
   Traditional Chinese medicine decoction enhances growth performance and intestinal
   glucose absorption in heat stressed pigs by up-regulating the expressions of SGLT1 and
   GLUT2 mRNA.
   Livestock Science 128 (2010) 75–81.
123.Yuan H, Zhang W, Du YZ, Hu FQ.
   Ternary nanoparticles of anionic lipid nanoparticles/protamine/DNA for gene delivery.
   Int J Pharm. 2010;392(1-2):224-31. Epub 2010 Mar 15.
124.Yu Q, Zhao Z, Sun J, Guo W, Fu J, Burnstock G, He C, Xiang Z.
   Expression of P2X6 receptors in the enteric nervous system of the rat gastrointestinal
   tract.
   Histochem Cell Biol. 2010;133(2):177-88. Epub 2009 Nov 28.
125.Hu H, Zhang Y, Zou M, Yang S, Liang XQ.
   Expression of TRF1, TRF2, TIN2, TERT, KU70, and BRCA1 proteins is associated with
   telomere shortening and may contribute to multistage carcinogenesis of gastric cancer.
   J Cancer Res Clin Oncol. 2010;136(9):1407-14. Epub 2010 Feb 2.
126.Ding XL, Wang YH, Ning LP, Zhang Y, Ge HY, Jiang H, Wang R, Yue SW.
   Involvement of TRPV4-NO-cGMP-PKG pathways in the development of thermal hyperalgesia
   following chronic compression of the dorsal root ganglion in rats.
   Behav Brain Res. 2010;208(1):194-201. Epub 2009 Dec 3.
127.Chen Z, Liu S, Sumida T, Sun S, Wei Y, Liu M, Dong Z, Zhang F, Hamakawa H, Wei F.
   Silencing Id-1 with RNA Interference Inhibits Adenoid Cystic Carcinoma in Mice.
   J Surg Res. 2010. [Epub ahead of print].
128.Zhang JC, Liu CL, Li YP, Sun J, Wang P, Di KQ, Chen H, Zhao YY .
   Effect of yttrium ion on the proliferation, differentiation and mineralization function
   of primary mouse osteoblasts in vitro.
   Journal Of Rare Earths. Vol. 28, No. 3, Jun. 2010, p. 466.
129.Huang LH, Hu JQ, Tao WQ, Li YH, Li GM, Xie PY, Liu XS, Jiang J.
   Gossypol inhibits phosphorylation of Bcl-2 in human leukemia HL-60 cells.
   Eur J Pharmacol. 2010;645(1-3):9-13. Epub 2010 Jul 13.
130.Sun J, Liu X, Li Q.
   Molecular cloning, expression and antioxidant activity of a peroxiredoxin 2 homologue
   from Lampetra japonica.
   Fish Shellfish Immunol. 2010;28(5-6):795-801. Epub 2010 Feb 6.
131.Wang S, Liu S, Liu H, Wang J, Zhou S, Jiang RJ, Bendena WG, Li S.
   20-hydroxyecdysone reduces insect food consumption resulting in fat body lipolysis
   during molting and pupation.
   J Mol Cell Biol. 2010;2(3):128-38. Epub 2010 Apr 29.
132.Cao BY, Yang YP, Luo WF, Mao CJ, Han R, Sun X, Cheng J, Liu CF.
   Paeoniflorin, a potent natural compound, protects PC12 cells from MPP+ and acidic damage
   via autophagic pathway.
   J Ethnopharmacol. 2010;131(1):122-9. Epub 2010 Jun 15.
133.Liu J, Wang A, Li L, Huang Y, Xue P, Hao A.
   Oxidative stress mediates hippocampal neuron death in rats after lithium-pilocarpine-
   induced status epilepticus.
   Seizure. 2010;19(3):165-72. Epub 2010 Feb 10.
134.Li C, Xing G, Dong M, Zhou L, Li J, Wang G, Zou D, Wang R, Liu J, Niu Y.
   Beta-asarone protection against beta-amyloid-induced neurotoxicity in PC12 cells via JNK
   signaling and modulation of Bcl-2 family proteins.
   Eur J Pharmacol. 2010;635(1-3):96-102. Epub 2010 Mar 20.
135.Wu W, Yan C, Gan T, Chen Z, Lu X, Duerksen-Hughes PJ, Zhu X, Yang J.
   Nuclear proteome analysis of cisplatin-treated HeLa cells.
   Mutat Res. 2010;691(1-2):1-8. Epub 2010 Jun 9.
136.Baohua Y, Li L.
   Effects of SIRT6 silencing on collagen metabolism in human dermal fibroblasts.
   Cell Biol Int. 2012 Jan;36(1):105-8.
137.Han X, Lu M, Wang S, Lv D, Liu H
   Targeting IKK/NF-κB pathway reduces infiltration of inflammatory cells and apoptosis
   after spinalcord injury in rats.
   Neurosci Lett. 2012 Mar 5;511(1):28-32.
138.Xiao O, Xie ZL, Lin BW, Yin XF, Pi RB, Zhou SY
   Minocycline inhibits alkali burn-induced corneal neovascularization in mice.
   PLoS One. 2012;7(7):e41858.
139.Liu Z, Zhang J, Huang X, Huang L, Li S, Wang Z
   Magnesium sulfate inhibits the secretion of high mobility group box 1 from
   lipopolysaccharide-activated RAW264.7 macrophages in vitro.
   J Surg Res. 2012 Mar 28.
140.Yang XH, Wang YH, Wang JJ, Liu YC, Deng W, Qin C, Gao JL, Zhang LY
   Role of angiotensin-converting enzyme (ACE and ACE2) imbalance on tourniquet-induced
   remotekidney injury in a mouse hindlimb ischemia-reperfusion model.
   Peptides. 2012 Jul;36(1):60-70.
141.Liu H, Chen X, Gao W, Jiang G.
   The expression of heparanase and microRNA-1258 in human non-small cell lung cancer.
   Tumour Biol. 2012 Apr 10.
142.Li TT, Zhang YS, He L, Liu B, Shi RZ, Zhang GG, Peng J
   Inhibition of vascular peroxidase alleviates cardiac dysfunction and apoptosis induced
   by ischemia-reperfusion.
   Can J Physiol Pharmacol. 2012 Jul;90(7):851-62.
143.Zhang L, Ding W, Sun H, Zhou Q, Huang J, Li X, Xie Y, Chen J
   Salidroside protects PC12 cells from MPP(+)-induced apoptosis via activation of the
   PI3K/Akt pathway.
   Food Chem Toxicol. 2012 Aug;50(8):2591-7.
144.Yu SJ, Liu HC, Ling-Ling E, Wang DS, Zhu GX
   Proliferation and differentiation of osteoblasts from the mandible of osteoporotic rats.
   Exp Biol Med (Maywood). 2012 Apr;237(4):395-406.
145.Jiang L, Lin J, Han H, Dong H, Zhao Q, Zhu S, Huang B.
   Identification and Characterization of Eimeria tenella Apical Membrane Antigen-1 (AMA1).
   PLoS One. 2012;7(7):e41115.
146.Chen M, Hu M, Wang D, Wang G, Zhu X, Yan D, Sun J.
   Multifunctional Hyperbranched Glycoconjugated Polymers Based on Natural Aminoglycosides.
   Bioconjug Chem. 2012 May 25.
147.Sun D, Wang Y, Liu C, Zhou X, Li X, Xiao A
   Effects of nitric oxide on renal interstitial fibrosis in rats with unilateral ureteral
   obstruction.
   Life Sci. 2012 Jun 14;90(23-24):900-9.
148.Zhang YS, He L, Liu B, Li NS, Luo XJ, Hu CP, Ma QL, Zhang GG, Li YJ, Peng J
   A novel pathway of NADPH oxidase/vascular peroxidase 1 in mediating oxidative injury
   followingischemia-reperfusion.
   Basic Res Cardiol. 2012;107(3):266. Epub 2012 Apr 3.
149.Zhu C, Wang Q, Xie J, Shi J, Zhou X, Li D, Xiong F, Zhang L
   Expression and Significance of RKIP and E-cadherin in Lung Squamous Cell Carcinoma.
   Pathol Oncol Res. 2012 Jun 13.
150.Zhao P, Han T, Guo JJ, Zhu SL, Wang J, Ao F, Jing MZ, She YL, Wu ZH, Ye LB.
   HCV NS4B induces apoptosis through the mitochondrial death pathway.
   Virus Res. 2012 Apr 19.
151.Zhang Y, Xu Y, Zhu Q, Zhao F, Luo J, Zhang X, Wang X
   Upregulation of dysbindin in temporal lobe epileptic foci of human and experimental
   animals.
   Synapse. 2012 Jul;66(7):622-9. doi: 10.1002/syn.21548. Epub 2012 Mar 30.
152.Zhang X, Xu Y, Wang J, Zhou Q, Pu S, Jiang W, Du D.
   The effect of intrathecal administration of glial activation inhibitors on dorsal horn
   BDNFoverexpression and hind paw mechanical allodynia in spinal nerve ligated rats.
   J Neural Transm. 2012 Mar;119(3):329-36.
153.Song Z, Liu L, Yue Y, Jiao H, Lin H, Sheikhahmadi A, Everaert N, Decuypere E, Buyse J.
   Fasting alters protein expression of AMP-activated protein kinase in the hypothalamus of
   broilerchicks (Gallus gallus domesticus).
   Gen Comp Endocrinol. 2012 Jul 5.
154.Lu N, Liu Q, Li R, Xie L, Shen J, Guan W, Qian X, Yu L, Ding Y, Jiang X, Liu B.
   Superior antimetastatic effect of pemetrexed-loaded gelatinase-responsive nanoparticles
   in amouse metastasis model.
   Anticancer Drugs. 2012 Jul 11. [Epub ahead of print]
155.Xu MF, Xiong YY, Liu JK, Qian JJ, Zhu L, Gao J
   Asiatic acid, a pentacyclic triterpene in Centella asiatica, attenuates
   glutamate-induced cognitivedeficits in mice and apoptosis in SH-SY5Y cells.
   Acta Pharmacol Sin. 2012 May;33(5):578-87.
156.Zhao XY, Zhu YJ, Chen F, Wu J.
   Calcium phosphate nanocarriers dual-loaded with bovine serum albumin and Ibuprofen:
   facilesynthesis, sequential drug loading and sustained drug release.
   Chem Asian J. 2012 Jun;7(7):1610-5.
157.Hu B, Zhou H, Gao H, Liu Y, Yan T, Zou L, Chen L.
   IFN-γ Inhibits Osteopontin Expression in Human Decidual Stromal Cells and can be
   Attenuated by 1α,25-Dihydroxyvitamin D3.
   Am J Reprod Immunol. 2012 Jul 11.
158.Yang L, Cheng F, Liu T, Lu JR, Song K, Jiang L, Wu S, Guo W
   Comparison of mesenchymal stem cells released from poly(N-isopropylacrylamide)
   copolymerfilm and by trypsinization.
   Biomed Mater. 2012 Jun;7(3):035003.
159.Zhang Q, He QF, Zhang TH, Yu XL, Liu Q, Deng FL
   Improvement in the delivery system of bone morphogenetic protein-2: a new approach to
   promotebone formation.
   Biomed Mater. 2012 Aug;7(4):045002.
160.Sun X, Cheng F, Meng B, Yang B, Song W, Yuan H
   Pregnenolone sulfate decreases intraocular pressure and changes expression of sigma
   receptorin a model of chronic ocular hypertension.
   Mol Biol Rep. 2012 Jun;39(6):6607-14.
161.Wang T, Fu F, Han B, Zhang L, Zhang X.
   Danshensu ameliorates the cognitive decline in streptozotocin-induced diabetic mice
   byattenuating advanced glycation end product-mediated neuroinflammation.
   J Neuroimmunol. 2012 Apr;245(1-2):79-86.
162.Ren M, Qin D, Li K, Qu J, Wang L, Wang Z, Huang A, Tang H
   Correlation between hepatitis B virus protein and microRNA processor Drosha in cells
   expressingHBV.
   Antiviral Res. 2012 Jun;94(3):225-31.
163.Zhang Y, Zhou L, Zhang X, Bai J, Shi M, Zhao G.
   Ginsenoside-Rd attenuates TRPM7 and ASIC1a but promotes ASIC2a expression in rats after
   focalcerebral ischemia.
   Neurol Sci. 2012 Jan 10.
164.Bi WY, Fu BD, Shen HQ, Wei Q, Zhang C, Song Z, Qin QQ, Li HP, Lv S, Wu SC, Yi PF,
   Wei XB
   Sulfated Derivative of 20(S)-Ginsenoside Rh2 Inhibits Inflammatory Cytokines Through
   MAPKs andNF-kappa B Pathways in LPS-Induced RAW264.7 Macrophages.
   Inflammation. 2012 May 22.
165.Yu H, Li Q, Zhou X, Kolosov VP, Perelman JM
   Transient receptor potential vanilloid 1 receptors mediate acid-induced mucin secretion
   via Ca2+influx in human airway epithelial cells.
   J Biochem Mol Toxicol. 2012 May;26(5):179-86.
166.Tian J, Yan J, Wang W, Zhong N, Tian L, Sun J, Min Z, Ma J, Lu S.
   T-2 toxin enhances catabolic activity of hypertrophic chondrocytes through
   ROS-NF-κB-HIF-2αpathway.
   Toxicol In Vitro. 2012 Jul 16.
167.Huang Q, Deng J, Xie Z, Wang F, Chen S, Lei B, Liao P, Huang N, Wang Z, Wang Z,
   Cheng Y.
   Effective gene transfer into central nervous system following
   ultrasound-microbubbles-inducedopening of the blood-brain barrier.
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168.Wu J, Lai G, Wan F, Xiao Z, Zeng L, Wang X, Ye F, Lei T.
   Knockdown of checkpoint kinase 1 is associated with the increased radiosensitivity
   ofglioblastoma stem-like cells.
   Tohoku J Exp Med. 2012;226(4):267-74.
169.Chen X, Yang X, Liu T, Guan M, Feng X, Dong W, Chu X, Liu J, Tian X, Ci X, Li H,
   Wei J, Deng Y, Deng X, Chi G, Sun Z.
   Kaempferol regulates MAPKs and NF-κB signaling pathways to attenuate LPS-induced acute
   lunginjury in mice.
   Int Immunopharmacol. 2012 Jul 23.
170.Fauzee NJ, Wang YL, Dong Z, Li QG, Wang T, Mandarry MT, Xu L, Pan J
   Novel hydrophilic docetaxel (CQMU-0519) analogue inhibits proliferation and induces
   apoptosis inhuman A549 lung, SKVO3 ovarian and MCF7 breast carcinoma cell lines.
   Cell Prolif. 2012 Aug;45(4):352-64.
171.Li XY, Jing CQ, Zang XY, Yang S, Wang JJ.
   Toxic cytological alteration and mitochondrial dysfunction in PC12 cells induced by
   1-octyl-3-methylimidazolium chloride.
   Toxicol In Vitro. 2012 Jul 23.
172.Zhou YM, Zhang XF, Cao L, Li B, Sui CJ, Li YM, Yin ZF
   MCM7 expression predicts post-operative prognosis for hepatocellular carcinoma.
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173.Lin N, Chen H, Zhang H, Wan X, Su Q.
   Mitochondrial reactive oxygen species (ROS) inhibition ameliorates palmitate-induced
   INS-1 betacell death.
   Endocrine. 2012 Aug;42(1):107-17.
174.Liu P, Shi Y, Yang Y, Cao Y, Shi Y, Li H, Liu J, Lin J, Jin Y.
   Schistosoma Japonicum UDP-Glucose 4-Epimerase Protein Is Located on the Tegument
   andInduces Moderate Protection against Challenge Infection.
   PLoS One. 2012;7(7):e42050.
175.Du HM, Zheng XH, Wang LY, Tang W, Liu L, Jing W, Lin YF, Tian WD, Long J
   The Osteogenic Response of Undifferentiated Human Adipose-Derived Stem Cells
   underMechanical Stimulation.
   Cells Tissues Organs. 2012 May 15.
176.Li XJ, Liu DP, Chen HL, Pan XH, Kong QY, Pang QF
   Lactoferrin protects against lipopolysaccharide-induced acute lung injury in mice.
   Int Immunopharmacol. 2012 Feb;12(2):460-4.
177.Zuo K, Li D, Pulli B, Yu F, Cai H, Yuan X, Zhang X, Lv Z.
   Short-hairpin RNA-mediated Heat shock protein 90 gene silencing inhibits human breast
   cancercell growth in vitro and in vivo.
   Biochem Biophys Res Commun. 2012 May 4;421(2):396-402.
178.Wang Y, Xie S, Liu C, Wu Y, Liu Y, Cai Y.
   Inhibitory effect of liquiritigenin on migration via downregulation proMMP-2 and
   PI3K/Akt signalingpathway in human lung adenocarcinoma A549 cells.
   Nutr Cancer. 2012;64(4):627-34.
179.Song Z, Li Y, Liu Y, Xin J, Zou X, Sun W.
   α-Enolase, an Adhesion-Related Factor of Mycoplasma bovis.
   PLoS One. 2012;7(6):e38836. Epub 2012 Jun 13.
180.Liu W, Li N.
   Chicken sine oculis binding protein homolog (sobp), a novel gene that may regulate
   feather development.
   Poult Sci. 2012 Aug;91(8):1950-5.
181.Ding Y, Huang X, Liu T, Fu Y, Tan Z, Zheng H, Zhou T, Dai J, Xu W.
   The Plasmodium Circumsporozoite Protein, a Novel NF-κB Inhibitor, Suppresses the Growth
   ofSW480.
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182.Zhong X, Li W, Huang X, Zhang L, Yimamu M, Raiput N, Zhou Y, Wang T.
   Impairment of cellular immunity is associated with overexpression of heat shock protein
   70 inneonatal pigs with intrauterine growth retardation.
   Cell Stress Chaperones. 2012 Jul;17(4):495-505.
183.Sang H, Zhang L, Li J
   Anti-benzopyrene-7,8-diol-9,10-epoxide induces apoptosis via mitochondrial pathway in
   humanbronchiolar epithelium cells independent of the mitochondria permeability
   transition pore.
   Food Chem Toxicol. 2012 Jul;50(7):2417-23.
184.Zhu XB, Wang YB, Chen O, Zhang DQ, Zhang ZH, Cao AH, Huang SY, Sun RP.
   Characterization of the expression of macrophage inflammatory protein-1α (MIP-1α)
   andchemokine receptor CCR5 after kainic acid-induced status epilepticus (SE) in juvenile
   rats.
   Neuropathol Appl Neurobiol. 2012 Jan 16.
185.Li Y, Wang J, Grebogi C, Foote M, Liu F.
   A syringe-focused ultrasound device for simultaneous injection of DNA and gene transfer.
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186.Hao M, Kuang HY, Fu Z, Gao XY, Liu Y, Deng W.
   Exenatide prevents high-glucose-induced damage of retinal ganglion cells through a
   mitochondrialmechanism.
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187.Zhao S, Zhao G, Xie H, Huang Y, Hou Y.
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